Fabrication of impedimetric sensors for labe-free Point-of-Care immunoassay cardiac marker systems with microfluidic blood flow delivery

M Tweedie, R Subramanian, P Lemoine, I Craig, ET McAdams, JAD McLaughlin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

14 Citations (Scopus)

Abstract

Miniaturised point-of-care cardiac marker sensors are being developed, based on impedimetric sensing of cardiac enzyme capture by an antibody layer immobilised on a planar gold electrode sensor. Gold/Ti-on-glass substrates have been used, in a 2 electrode configuration, with antibodies immobilised on the working electrode. Microfluidic structures have been fabricated by a CO2 laser, in 25 mum thick pressure sensitive adhesive (PSA), on a PMMA lid, and the structure bonded on top of the planar sensor. Microfluidic blood/serum delivery has been investigated using a visualisation dye. Some flow problems are observed if the sensor is exposed to air for several days, suggesting that flow channel nanopillars and hermetic encapsulation may be required to guarantee flow properties in commercially produced modules. Work is ongoing to characterise the impedimetric signal changes for myoglobin capture by antimyoglobin, using these sensors. Fifty micron thick PSA, incorporating a robust spacer layer, will be used to give better definition of channel walls
LanguageEnglish
Title of host publicationUnknown Host Publication
Pages4610-4614
Number of pages5
DOIs
Publication statusPublished - 2006
EventEMBS 2006 - New York, USA
Duration: 1 Jan 2006 → …

Conference

ConferenceEMBS 2006
Period1/01/06 → …

Fingerprint

Microfluidics
Blood
Fabrication
Sensors
Antibodies
Electrodes
Adhesives
Gold
Channel flow
Encapsulation
Visualization
Enzymes
Dyes
Glass
Lasers
Substrates
Air

Keywords

  • Impedimetric antibody cardiac microfluidic planar point-of-care sensor

Cite this

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title = "Fabrication of impedimetric sensors for labe-free Point-of-Care immunoassay cardiac marker systems with microfluidic blood flow delivery",
abstract = "Miniaturised point-of-care cardiac marker sensors are being developed, based on impedimetric sensing of cardiac enzyme capture by an antibody layer immobilised on a planar gold electrode sensor. Gold/Ti-on-glass substrates have been used, in a 2 electrode configuration, with antibodies immobilised on the working electrode. Microfluidic structures have been fabricated by a CO2 laser, in 25 mum thick pressure sensitive adhesive (PSA), on a PMMA lid, and the structure bonded on top of the planar sensor. Microfluidic blood/serum delivery has been investigated using a visualisation dye. Some flow problems are observed if the sensor is exposed to air for several days, suggesting that flow channel nanopillars and hermetic encapsulation may be required to guarantee flow properties in commercially produced modules. Work is ongoing to characterise the impedimetric signal changes for myoglobin capture by antimyoglobin, using these sensors. Fifty micron thick PSA, incorporating a robust spacer layer, will be used to give better definition of channel walls",
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Fabrication of impedimetric sensors for labe-free Point-of-Care immunoassay cardiac marker systems with microfluidic blood flow delivery. / Tweedie, M; Subramanian, R; Lemoine, P; Craig, I; McAdams, ET; McLaughlin, JAD.

Unknown Host Publication. 2006. p. 4610-4614.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - Miniaturised point-of-care cardiac marker sensors are being developed, based on impedimetric sensing of cardiac enzyme capture by an antibody layer immobilised on a planar gold electrode sensor. Gold/Ti-on-glass substrates have been used, in a 2 electrode configuration, with antibodies immobilised on the working electrode. Microfluidic structures have been fabricated by a CO2 laser, in 25 mum thick pressure sensitive adhesive (PSA), on a PMMA lid, and the structure bonded on top of the planar sensor. Microfluidic blood/serum delivery has been investigated using a visualisation dye. Some flow problems are observed if the sensor is exposed to air for several days, suggesting that flow channel nanopillars and hermetic encapsulation may be required to guarantee flow properties in commercially produced modules. Work is ongoing to characterise the impedimetric signal changes for myoglobin capture by antimyoglobin, using these sensors. Fifty micron thick PSA, incorporating a robust spacer layer, will be used to give better definition of channel walls

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