FABRICATION OF BIOCOMPATIBLE NANO-CARBONATED HYDROXYAPATITE/POLYMER SPONGY SCAFFOLDS.

SA Siddiqi; U Azhar; F Manzoor; A Jamal; M Tariq; M Saleem; AA Chaudhry; IU Rehman

FABRICATION OF BIOCOMPATIBLE NANO-CARBONATED HYDROXYAPATITE/POLYMER SPONGY SCAFFOLDS.

SA Siddiqi, U Azhar, F Manzoor, A Jamal, M Tariq, M Saleem, AA Chaudhry, IU Rehman

Research output: Contribution to journal › Article › peer-review

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Abstract

Spongy scaffolds containing nano-carbonated hydroxyapatite (nCHA), with compositions Ca₁₀(PO₄)_6-y(CO₃)_y(OH)₂, with y=0,2,4, were prepared by the lyophilization method using different polymers such as natural gelatin (GE), chitosan (CS) and polyvinyl alcohol (PVA), alone or in their combinations. The samples were characterized by using XRD, SEM, and FTIR techniques. All the scaffolds were found to have porosities above 70% and their morphology changed significantly for one nCHA/polymer combination to the other. The nCHA containing scaffolds mediated cytotoxicity (MTT assay) and cell attachment studies were carried out on rat bone marrow derived mesenchymal stem cells
(rMSC). Evident cell attachment and no significant cytotoxicity was observed. The
preparation of spongy scaffolds and their biocompatibility augment their potential use in tissue engineering and biomedical applications.

Original language	English
Pages (from-to)	439-450
Number of pages	12
Journal	Digest Journal of Nanomaterials & Biostructures (DJNB)
Volume	13
Issue number	2
Early online date	Jun 2018
Publication status	Published online - Jun 2018

Keywords

Nano-carbonated hydroxyapatite
Spongy scaffolds
Mesenchymal stem cells
Hydroxyapatite/polymer scaffolds
MTT Assay
Biocompatability

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title = "FABRICATION OF BIOCOMPATIBLE NANO-CARBONATED HYDROXYAPATITE/POLYMER SPONGY SCAFFOLDS.",

abstract = "Spongy scaffolds containing nano-carbonated hydroxyapatite (nCHA), with compositions Ca10(PO4)6-y(CO3)y(OH)2, with y=0,2,4, were prepared by the lyophilization method using different polymers such as natural gelatin (GE), chitosan (CS) and polyvinyl alcohol (PVA), alone or in their combinations. The samples were characterized by using XRD, SEM, and FTIR techniques. All the scaffolds were found to have porosities above 70% and their morphology changed significantly for one nCHA/polymer combination to the other. The nCHA containing scaffolds mediated cytotoxicity (MTT assay) and cell attachment studies were carried out on rat bone marrow derived mesenchymal stem cells(rMSC). Evident cell attachment and no significant cytotoxicity was observed. Thepreparation of spongy scaffolds and their biocompatibility augment their potential use in tissue engineering and biomedical applications.",

keywords = "Nano-carbonated hydroxyapatite, Spongy scaffolds, Mesenchymal stem cells, Hydroxyapatite/polymer scaffolds, MTT Assay, Biocompatability",

author = "SA Siddiqi and U Azhar and F Manzoor and A Jamal and M Tariq and M Saleem and AA Chaudhry and IU Rehman",

year = "2018",

month = jun,

language = "English",

volume = "13",

pages = "439--450",

journal = "Digest Journal of Nanomaterials & Biostructures (DJNB)",

issn = "1842-3582",

publisher = "National Institute R and D of Materials Physics",

number = "2",

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T1 - FABRICATION OF BIOCOMPATIBLE NANO-CARBONATED HYDROXYAPATITE/POLYMER SPONGY SCAFFOLDS.

AU - Siddiqi, SA

AU - Azhar, U

AU - Manzoor, F

AU - Jamal, A

AU - Tariq, M

AU - Saleem, M

AU - Chaudhry, AA

AU - Rehman, IU

PY - 2018/6

Y1 - 2018/6

N2 - Spongy scaffolds containing nano-carbonated hydroxyapatite (nCHA), with compositions Ca10(PO4)6-y(CO3)y(OH)2, with y=0,2,4, were prepared by the lyophilization method using different polymers such as natural gelatin (GE), chitosan (CS) and polyvinyl alcohol (PVA), alone or in their combinations. The samples were characterized by using XRD, SEM, and FTIR techniques. All the scaffolds were found to have porosities above 70% and their morphology changed significantly for one nCHA/polymer combination to the other. The nCHA containing scaffolds mediated cytotoxicity (MTT assay) and cell attachment studies were carried out on rat bone marrow derived mesenchymal stem cells(rMSC). Evident cell attachment and no significant cytotoxicity was observed. Thepreparation of spongy scaffolds and their biocompatibility augment their potential use in tissue engineering and biomedical applications.

AB - Spongy scaffolds containing nano-carbonated hydroxyapatite (nCHA), with compositions Ca10(PO4)6-y(CO3)y(OH)2, with y=0,2,4, were prepared by the lyophilization method using different polymers such as natural gelatin (GE), chitosan (CS) and polyvinyl alcohol (PVA), alone or in their combinations. The samples were characterized by using XRD, SEM, and FTIR techniques. All the scaffolds were found to have porosities above 70% and their morphology changed significantly for one nCHA/polymer combination to the other. The nCHA containing scaffolds mediated cytotoxicity (MTT assay) and cell attachment studies were carried out on rat bone marrow derived mesenchymal stem cells(rMSC). Evident cell attachment and no significant cytotoxicity was observed. Thepreparation of spongy scaffolds and their biocompatibility augment their potential use in tissue engineering and biomedical applications.

KW - Nano-carbonated hydroxyapatite

KW - Spongy scaffolds

KW - Mesenchymal stem cells

KW - Hydroxyapatite/polymer scaffolds

KW - MTT Assay

KW - Biocompatability

M3 - Article

SN - 1842-3582

VL - 13

SP - 439

EP - 450

JO - Digest Journal of Nanomaterials & Biostructures (DJNB)

JF - Digest Journal of Nanomaterials & Biostructures (DJNB)

IS - 2

ER -

FABRICATION OF BIOCOMPATIBLE NANO-CARBONATED HYDROXYAPATITE/POLYMER SPONGY SCAFFOLDS.

Abstract

Keywords

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