Extraction of triterpenoid compounds from Ganoderma Lucidum spore powder through a dual-mode sonication process

Shuang Fei Shen, Li Fang Zhu, Zijing Wu, Guangkun Wang, Zeeshan Ahmad, Ming Wei Chang

    Research output: Contribution to journalArticle

    Abstract

    Development of drug products from natural sources enable advantageous treatment and therapy options. Bioactive compounds in Ganoderma lucidum spore powder (GLSP) are known for vast antibacterial, antioxidant and anti-cancer properties. Herein, we studied the use of dual-probe ultrasound to extract triterpenoids from GLSP and further investigated the bioactivity of resulting products. FTIR results confirm the presence of key peaks although dual-probe ultrasound varied extraction efficacy. Response surface methodology (RSM) was used to optimize extraction conditions (55:28 for solvent to solid ratio, 10.38 s of ultrasound time and 94% v/v of ethanol concentration). HPLC-Q-TOF-MS confirmed the presence of nine different compounds and in vitro tests confirm good biocompatibility. Extracts are shown to inhibit DPPH radicals, reaching a maximum (61.09 ± 1.38%) at triterpenoid concentrations of 600 µg/mL. Dual-mode assisted extraction provides an enhanced approach for active embedded fiber production on a scale favorable to industry when using optimized process parameters. Furthermore, triterpenoid extracts show antibacterial properties on Staphylococcus aureus and Escherichia coli with potential in antibacterial and anticancer applications.

    Original languageEnglish
    Pages (from-to)963-974
    Number of pages12
    JournalDrug Development and Industrial Pharmacy
    Volume46
    Issue number6
    Early online date13 May 2020
    DOIs
    Publication statusE-pub ahead of print - 13 May 2020

    Keywords

    • antibacterial
    • antioxidant
    • biocompatibility
    • dual-model ultrasound
    • Triterpenoids

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