Expression and modification proteomics during skeletal muscle ageing

Martin A Baraibar, Marine Gueugneau, Stephanie Duguez, Gillian Butler-Browne, Daniel Bechet, Bertrand Friguet

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Skeletal muscle ageing is characterized by a progressive and dramatic loss of muscle mass and strength leading to decreased muscular function resulting in muscle weakness which is often referred to as sarcopenia. Following the standardisation of "omics" approaches to study the genome (genomics) and the transcriptome (transcriptomics), the study of the proteins encoded by the genome, referred to as proteomics, is a tremendous challenge. Unlike the genome, the proteome varies in response to many physiological or pathological factors. In addition, the proteome is orders of magnitude more complex than the transcriptome due to post-translational modifications, protein oxidation and limited protein degradation. Proteomic studies, including the analysis of protein abundance as well as post-translational modified proteins have been shown to provide valuable information to unravel the key molecular pathways implicated in complex biological processes, such as tissue and organ ageing. In this article, we will describe proteomic approaches for the analysis of protein abundance as well as the specific protein targets for oxidative damage upon oxidative stress and/or during skeletal muscle ageing.
Original languageEnglish
Pages (from-to)339-352
JournalBiogerontology
Volume14
Issue number3
Early online date28 Apr 2013
DOIs
Publication statusE-pub ahead of print - 28 Apr 2013

Keywords

  • 2D-gel based expression proteomics Skeletal muscle Ageing Oxidative stress Protein oxidation Oxidative proteome modifications

Fingerprint Dive into the research topics of 'Expression and modification proteomics during skeletal muscle ageing'. Together they form a unique fingerprint.

  • Cite this

    Baraibar, M. A., Gueugneau, M., Duguez, S., Butler-Browne, G., Bechet, D., & Friguet, B. (2013). Expression and modification proteomics during skeletal muscle ageing. Biogerontology, 14(3), 339-352. https://doi.org/10.1007/s10522-013-9426-7