Exposure of bovine oocytes and embryos to elevated non-esterified fatty acid concentrations: integration of epigenetic and transcriptomic signatures in resultant blastocysts.

K. L. J Desmet, V. Van Hoeck, D Gagné, E. Fournier, A. Thakur, A. M. O' Doherty, CP Walsh, M. A. Sirad, P. E. J. Bols, J. L. M. R. Leroy

Research output: Contribution to journalArticle

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Abstract

Metabolic stress associated with negative energy balance in high producing dairy cattle and obesity in women is a risk factor for decreased fertility. Non-esterified fatty acids (NEFA) are involved in this pathogenesis as they jeopardize oocyte and embryo development. Growing evidence indicates that maternal metabolic disorders can disturb epigenetic programming, such as DNA methylation, in the offspring. Oocyte maturation and early embryo development coincide with methylation changes and both are sensitive to adverse environments. Therefore, we investigated whether elevated NEFA concentrations affect establishment and maintenance of DNA methylation in oocytes and embryos, subsequently altering transcriptomic profiles and developmental competence of resultant blastocysts.
Original languageEnglish
Pages (from-to)1-18
JournalBMC Genomics
Volume17
Issue number1004
DOIs
Publication statusPublished - 8 Dec 2016

Keywords

  • Oocyte
  • Embryo
  • Fertility
  • Free fatty acids
  • Maternal metabolism
  • DNA methylation
  • Epigenetics

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