EVIDENCE FOR ADENINE METHYLATION WITHIN THE MOUSE MYOGENIC GENE MYO-D1

PH KAY, E PEREIRA, SA MARLOW, G TURBETT, Christopher Mitchell, PF JACOBSEN, R HOLLIDAY, JM PAPADIMITRIOU

Research output: Contribution to journalArticle

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Abstract

Previous studies have indicated that there may be uncleavable TaqI sites (TCGA) within the mouse myogenic gene, Myo-D1, Fragments of DNA bearing most of the presumed insensitive TaqI sites have been reproduced using PCR. The presence of each of the originally uncleavable TaqI sites has been confirmed and each TaqI site has been shown to be sensitive to TaqI hydrolysis in PCR-synthesised genomic DNA. Since TaqI is inhibited by methylation of the adenine residue within its recognition sequence (but not by cytosine methylation), it is suggested that specific adenine bases are methylated in the coding region of Myo-DI and maintained throughout cell division. The same TaqI recognition sequences are insensitive to digestion in genomic DNA isolated from various mouse tissues including fetus, regenerating skeletal muscle and a myogenic cell line, all of which express Myo-D1. Thus, adenine methylation is not a modification of DNA following gametic fusion nor does it appear to play a major role in regulation of Myo-D1 expression.
LanguageEnglish
Pages89-95
JournalGene
Volume151
Issue number1-2
Publication statusPublished - Dec 1994

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Adenine
Methylation
DNA
Genes
Polymerase Chain Reaction
Cytosine
Cell Division
Digestion
Skeletal Muscle
Fetus
Hydrolysis
Cell Line

Cite this

KAY, PH., PEREIRA, E., MARLOW, SA., TURBETT, G., Mitchell, C., JACOBSEN, PF., ... PAPADIMITRIOU, JM. (1994). EVIDENCE FOR ADENINE METHYLATION WITHIN THE MOUSE MYOGENIC GENE MYO-D1. Gene, 151(1-2), 89-95.
KAY, PH ; PEREIRA, E ; MARLOW, SA ; TURBETT, G ; Mitchell, Christopher ; JACOBSEN, PF ; HOLLIDAY, R ; PAPADIMITRIOU, JM. / EVIDENCE FOR ADENINE METHYLATION WITHIN THE MOUSE MYOGENIC GENE MYO-D1. In: Gene. 1994 ; Vol. 151, No. 1-2. pp. 89-95.
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abstract = "Previous studies have indicated that there may be uncleavable TaqI sites (TCGA) within the mouse myogenic gene, Myo-D1, Fragments of DNA bearing most of the presumed insensitive TaqI sites have been reproduced using PCR. The presence of each of the originally uncleavable TaqI sites has been confirmed and each TaqI site has been shown to be sensitive to TaqI hydrolysis in PCR-synthesised genomic DNA. Since TaqI is inhibited by methylation of the adenine residue within its recognition sequence (but not by cytosine methylation), it is suggested that specific adenine bases are methylated in the coding region of Myo-DI and maintained throughout cell division. The same TaqI recognition sequences are insensitive to digestion in genomic DNA isolated from various mouse tissues including fetus, regenerating skeletal muscle and a myogenic cell line, all of which express Myo-D1. Thus, adenine methylation is not a modification of DNA following gametic fusion nor does it appear to play a major role in regulation of Myo-D1 expression.",
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KAY, PH, PEREIRA, E, MARLOW, SA, TURBETT, G, Mitchell, C, JACOBSEN, PF, HOLLIDAY, R & PAPADIMITRIOU, JM 1994, 'EVIDENCE FOR ADENINE METHYLATION WITHIN THE MOUSE MYOGENIC GENE MYO-D1', Gene, vol. 151, no. 1-2, pp. 89-95.

EVIDENCE FOR ADENINE METHYLATION WITHIN THE MOUSE MYOGENIC GENE MYO-D1. / KAY, PH; PEREIRA, E; MARLOW, SA; TURBETT, G; Mitchell, Christopher; JACOBSEN, PF; HOLLIDAY, R; PAPADIMITRIOU, JM.

In: Gene, Vol. 151, No. 1-2, 12.1994, p. 89-95.

Research output: Contribution to journalArticle

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AU - Mitchell, Christopher

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AB - Previous studies have indicated that there may be uncleavable TaqI sites (TCGA) within the mouse myogenic gene, Myo-D1, Fragments of DNA bearing most of the presumed insensitive TaqI sites have been reproduced using PCR. The presence of each of the originally uncleavable TaqI sites has been confirmed and each TaqI site has been shown to be sensitive to TaqI hydrolysis in PCR-synthesised genomic DNA. Since TaqI is inhibited by methylation of the adenine residue within its recognition sequence (but not by cytosine methylation), it is suggested that specific adenine bases are methylated in the coding region of Myo-DI and maintained throughout cell division. The same TaqI recognition sequences are insensitive to digestion in genomic DNA isolated from various mouse tissues including fetus, regenerating skeletal muscle and a myogenic cell line, all of which express Myo-D1. Thus, adenine methylation is not a modification of DNA following gametic fusion nor does it appear to play a major role in regulation of Myo-D1 expression.

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KAY PH, PEREIRA E, MARLOW SA, TURBETT G, Mitchell C, JACOBSEN PF et al. EVIDENCE FOR ADENINE METHYLATION WITHIN THE MOUSE MYOGENIC GENE MYO-D1. Gene. 1994 Dec;151(1-2):89-95.