EVIDENCE FOR ADENINE METHYLATION WITHIN THE MOUSE MYOGENIC GENE MYO-D1

PH KAY, E PEREIRA, SA MARLOW, G TURBETT, Christopher Mitchell, PF JACOBSEN, R HOLLIDAY, JM PAPADIMITRIOU

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Previous studies have indicated that there may be uncleavable TaqI sites (TCGA) within the mouse myogenic gene, Myo-D1, Fragments of DNA bearing most of the presumed insensitive TaqI sites have been reproduced using PCR. The presence of each of the originally uncleavable TaqI sites has been confirmed and each TaqI site has been shown to be sensitive to TaqI hydrolysis in PCR-synthesised genomic DNA. Since TaqI is inhibited by methylation of the adenine residue within its recognition sequence (but not by cytosine methylation), it is suggested that specific adenine bases are methylated in the coding region of Myo-DI and maintained throughout cell division. The same TaqI recognition sequences are insensitive to digestion in genomic DNA isolated from various mouse tissues including fetus, regenerating skeletal muscle and a myogenic cell line, all of which express Myo-D1. Thus, adenine methylation is not a modification of DNA following gametic fusion nor does it appear to play a major role in regulation of Myo-D1 expression.
Original languageEnglish
Pages (from-to)89-95
JournalGene
Volume151
Issue number1-2
Publication statusPublished - Dec 1994

Fingerprint Dive into the research topics of 'EVIDENCE FOR ADENINE METHYLATION WITHIN THE MOUSE MYOGENIC GENE MYO-D1'. Together they form a unique fingerprint.

  • Cite this

    KAY, PH., PEREIRA, E., MARLOW, SA., TURBETT, G., Mitchell, C., JACOBSEN, PF., HOLLIDAY, R., & PAPADIMITRIOU, JM. (1994). EVIDENCE FOR ADENINE METHYLATION WITHIN THE MOUSE MYOGENIC GENE MYO-D1. Gene, 151(1-2), 89-95.