Evaluation of the insulinotropic and glucose-lowering actions of zebrafish GIP in mammalian systems: Evidence for involvement of the GLP-1 receptor.

GV Graham; JM Conlon; YHA Abdel-Wahab; Victor A Gault; Peter Flatt

doi:10.1016/j.peptides.2017.11.007

Evaluation of the insulinotropic and glucose-lowering actions of zebrafish GIP in mammalian systems: Evidence for involvement of the GLP-1 receptor.

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Abstract

The insulinotropic properties of zebrafish GIP (zfGIP) were assessed in vitro using clonal pancreatic β-cell lines and isolated mouse islets and acute effects on glucose tolerance and insulin release in vivo were evaluated in mice. The peptide produced a dose-dependent increase in the rate of insulin release from BRIN-BD11 rat clonal β-cells at concentrations ≥30nM. Insulin release from 1.1 B4 human clonal β-cells and mouse islets was significantly increased by zfGIP (10nM and 1μM). The in vitro insulinotropic activity of zfGIP was decreased after incubating BRIN-BD11 cells with the GLP-1 receptor antagonist, exendin-4(9-39) (p

Original language	English
Pages (from-to)	182-189
Journal	Peptides
Volume	100
Early online date	20 Nov 2017
DOIs	https://doi.org/10.1016/j.peptides.2017.11.007
Publication status	Published online - 20 Nov 2017

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Type 2 diabetes
Zebrafish
GIP
GLP-1
Glucagon
Evolution

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10.1016/j.peptides.2017.11.007

Insulinotropic and glucose-lowering peptides from phylogenetically ancient fish with potential for therapy of type 2 diabetes
Graham, G. (Author), Flatt, P. (Supervisor), Abdel-Wahab, Y. (Supervisor) & Conlon, J. (Supervisor), Apr 2019
Student thesis: Doctoral Thesis

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abstract = "The insulinotropic properties of zebrafish GIP (zfGIP) were assessed in vitro using clonal pancreatic β-cell lines and isolated mouse islets and acute effects on glucose tolerance and insulin release in vivo were evaluated in mice. The peptide produced a dose-dependent increase in the rate of insulin release from BRIN-BD11 rat clonal β-cells at concentrations ≥30nM. Insulin release from 1.1 B4 human clonal β-cells and mouse islets was significantly increased by zfGIP (10nM and 1μM). The in vitro insulinotropic activity of zfGIP was decreased after incubating BRIN-BD11 cells with the GLP-1 receptor antagonist, exendin-4(9-39) (p",

keywords = "Type 2 diabetes, Zebrafish, GIP, GLP-1, Glucagon, Evolution",

author = "GV Graham and JM Conlon and YHA Abdel-Wahab and Gault, \{Victor A\} and Peter Flatt",

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AU - Gault, Victor A

AU - Flatt, Peter

PY - 2017/11/20

Y1 - 2017/11/20

N2 - The insulinotropic properties of zebrafish GIP (zfGIP) were assessed in vitro using clonal pancreatic β-cell lines and isolated mouse islets and acute effects on glucose tolerance and insulin release in vivo were evaluated in mice. The peptide produced a dose-dependent increase in the rate of insulin release from BRIN-BD11 rat clonal β-cells at concentrations ≥30nM. Insulin release from 1.1 B4 human clonal β-cells and mouse islets was significantly increased by zfGIP (10nM and 1μM). The in vitro insulinotropic activity of zfGIP was decreased after incubating BRIN-BD11 cells with the GLP-1 receptor antagonist, exendin-4(9-39) (p

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KW - Zebrafish

KW - GIP

KW - GLP-1

KW - Glucagon

KW - Evolution

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JO - Peptides

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Evaluation of the insulinotropic and glucose-lowering actions of zebrafish GIP in mammalian systems: Evidence for involvement of the GLP-1 receptor.

Abstract

UN SDGs

Keywords

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Student theses

Insulinotropic and glucose-lowering peptides from phylogenetically ancient fish with potential for therapy of type 2 diabetes

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