Abstract
The insulinotropic properties of zebrafish GIP (zfGIP) were assessed in vitro using clonal pancreatic β-cell lines and isolated mouse islets and acute effects on glucose tolerance and insulin release in vivo were evaluated in mice. The peptide produced a dose-dependent increase in the rate of insulin release from BRIN-BD11 rat clonal β-cells at concentrations ≥30nM. Insulin release from 1.1 B4 human clonal β-cells and mouse islets was significantly increased by zfGIP (10nM and 1μM). The in vitro insulinotropic activity of zfGIP was decreased after incubating BRIN-BD11 cells with the GLP-1 receptor antagonist, exendin-4(9-39) (p
Original language | English |
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Pages (from-to) | 182-189 |
Journal | Peptides |
Volume | 100 |
Early online date | 20 Nov 2017 |
DOIs | |
Publication status | Published online - 20 Nov 2017 |
Keywords
- Type 2 diabetes
- Zebrafish
- GIP
- GLP-1
- Glucagon
- Evolution