Evaluation of glycated insulin in diabetic animals using immunocytochemistry and radioimmunoassay

Aine McKillop, MH Mooney, P Harriott, Peter Flatt, Finbarr O'Harte

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Abstract

Glycated insulin was evaluated in plasma and biological tissues of diabetic animal models by immuno. cytochemistry (ICC) and a novel radioimmunoassay. Glycated insulin circulated at 0.10 +/-0.04 ng/ml and 2.20 +/-0.14 ng/ml in lean and diabetic obese (ob/ob) mice, corresponding to 12.5 and 9.8% total plasma insulin, respectively. The concentration of glycated insulin was elevated 22-fold in obese mice compared to controls (P
LanguageEnglish
Pages524-528
JournalBiochemical and Biophysical Research Communications
Volume286
Issue number3
Publication statusPublished - Aug 2001

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Radioimmunoassay
Immunohistochemistry
Obese Mice
Histocytochemistry
Animal Models
Insulin
glycosylated insulin

Cite this

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AU - Mooney, MH

AU - Harriott, P

AU - Flatt, Peter

AU - O'Harte, Finbarr

PY - 2001/8

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AB - Glycated insulin was evaluated in plasma and biological tissues of diabetic animal models by immuno. cytochemistry (ICC) and a novel radioimmunoassay. Glycated insulin circulated at 0.10 +/-0.04 ng/ml and 2.20 +/-0.14 ng/ml in lean and diabetic obese (ob/ob) mice, corresponding to 12.5 and 9.8% total plasma insulin, respectively. The concentration of glycated insulin was elevated 22-fold in obese mice compared to controls (P

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EP - 528

JO - Biochemical and Biophysical Research Communications

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JF - Biochemical and Biophysical Research Communications

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