ETHANOL PRODUCTION FROM SUGARCANE BAGASSE USING SSF PROCESS AND THERMOTOLERANT YEAST

G. M. Silva, R. L. C. Giordano, A. J. G. Cruz, K.D Ramachandriya, Ibrahim Banat, M. R. Wilkins

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Sugarcane bagasse (SCB) pretreated by hydrothermolysis at 195°C for 10 min was used as a substrate for ethanol production in a simultaneous saccharification and fermentation (SSF) process with the thermotolerant yeast Kluyveromyces marxianus IMB3 at 45°C. SSF was carried out for 7 days using 15 and 30 filter paper units (FPU) cellulase g-1 treated SCB (Accellerase 1500) to determine the effect of enzyme loading on ethanol production. Different pretreated dry solids loadings of 10% and 15% (w/w) were investigated in this work during enzymatic hydrolysis. Results showed 89.7% hemicellulose removal during pretreatment and glucan recovery of 97.8%. The highest ethanol concentration of 29.2 g L-1 was obtained with 30 FPU cellulase g-1 treated SCB within 72 h, which was equivalent to 58.9% of the maximum theoretical ethanol yield.
LanguageEnglish
Pages193-200
JournalTransactions of the ASABE
Volume58
Issue number2
Publication statusPublished - 2015

Fingerprint

sugarcane bagasse
saccharification
ethanol production
fermentation
yeasts
endo-1,4-beta-glucanase
ethanol
Kluyveromyces marxianus
glucans
enzymatic hydrolysis
hemicellulose
pretreatment
enzymes

Keywords

  • Enzymatic hydrolysis
  • Ethanol
  • Hydrothermolysis
  • Kluyveromyces marxianus IMB3
  • SSF
  • Sugarcane bagasse

Cite this

Silva, G. M., Giordano, R. L. C., Cruz, A. J. G., Ramachandriya, K. D., Banat, I., & Wilkins, M. R. (2015). ETHANOL PRODUCTION FROM SUGARCANE BAGASSE USING SSF PROCESS AND THERMOTOLERANT YEAST. 58(2), 193-200.
Silva, G. M. ; Giordano, R. L. C. ; Cruz, A. J. G. ; Ramachandriya, K.D ; Banat, Ibrahim ; Wilkins, M. R. / ETHANOL PRODUCTION FROM SUGARCANE BAGASSE USING SSF PROCESS AND THERMOTOLERANT YEAST. 2015 ; Vol. 58, No. 2. pp. 193-200.
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Silva, GM, Giordano, RLC, Cruz, AJG, Ramachandriya, KD, Banat, I & Wilkins, MR 2015, 'ETHANOL PRODUCTION FROM SUGARCANE BAGASSE USING SSF PROCESS AND THERMOTOLERANT YEAST', vol. 58, no. 2, pp. 193-200.

ETHANOL PRODUCTION FROM SUGARCANE BAGASSE USING SSF PROCESS AND THERMOTOLERANT YEAST. / Silva, G. M.; Giordano, R. L. C.; Cruz, A. J. G.; Ramachandriya, K.D; Banat, Ibrahim; Wilkins, M. R.

Vol. 58, No. 2, 2015, p. 193-200.

Research output: Contribution to journalArticle

TY - JOUR

T1 - ETHANOL PRODUCTION FROM SUGARCANE BAGASSE USING SSF PROCESS AND THERMOTOLERANT YEAST

AU - Silva, G. M.

AU - Giordano, R. L. C.

AU - Cruz, A. J. G.

AU - Ramachandriya, K.D

AU - Banat, Ibrahim

AU - Wilkins, M. R.

PY - 2015

Y1 - 2015

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AB - Sugarcane bagasse (SCB) pretreated by hydrothermolysis at 195°C for 10 min was used as a substrate for ethanol production in a simultaneous saccharification and fermentation (SSF) process with the thermotolerant yeast Kluyveromyces marxianus IMB3 at 45°C. SSF was carried out for 7 days using 15 and 30 filter paper units (FPU) cellulase g-1 treated SCB (Accellerase 1500) to determine the effect of enzyme loading on ethanol production. Different pretreated dry solids loadings of 10% and 15% (w/w) were investigated in this work during enzymatic hydrolysis. Results showed 89.7% hemicellulose removal during pretreatment and glucan recovery of 97.8%. The highest ethanol concentration of 29.2 g L-1 was obtained with 30 FPU cellulase g-1 treated SCB within 72 h, which was equivalent to 58.9% of the maximum theoretical ethanol yield.

KW - Enzymatic hydrolysis

KW - Ethanol

KW - Hydrothermolysis

KW - Kluyveromyces marxianus IMB3

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Silva GM, Giordano RLC, Cruz AJG, Ramachandriya KD, Banat I, Wilkins MR. ETHANOL PRODUCTION FROM SUGARCANE BAGASSE USING SSF PROCESS AND THERMOTOLERANT YEAST. 2015;58(2):193-200.