ETHANOL PRODUCTION FROM SUGARCANE BAGASSE USING SSF PROCESS AND THERMOTOLERANT YEAST

G. M. Silva, R. L. C. Giordano, A. J. G. Cruz, K.D Ramachandriya, Ibrahim Banat, M. R. Wilkins

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Abstract

Sugarcane bagasse (SCB) pretreated by hydrothermolysis at 195°C for 10 min was used as a substrate for ethanol production in a simultaneous saccharification and fermentation (SSF) process with the thermotolerant yeast Kluyveromyces marxianus IMB3 at 45°C. SSF was carried out for 7 days using 15 and 30 filter paper units (FPU) cellulase g-1 treated SCB (Accellerase 1500) to determine the effect of enzyme loading on ethanol production. Different pretreated dry solids loadings of 10% and 15% (w/w) were investigated in this work during enzymatic hydrolysis. Results showed 89.7% hemicellulose removal during pretreatment and glucan recovery of 97.8%. The highest ethanol concentration of 29.2 g L-1 was obtained with 30 FPU cellulase g-1 treated SCB within 72 h, which was equivalent to 58.9% of the maximum theoretical ethanol yield.
Original languageEnglish
Pages (from-to)193-200
JournalTransactions of the ASABE
Volume58
Issue number2
Publication statusPublished - 2015

Keywords

  • Enzymatic hydrolysis
  • Ethanol
  • Hydrothermolysis
  • Kluyveromyces marxianus IMB3
  • SSF
  • Sugarcane bagasse

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    Silva, G. M., Giordano, R. L. C., Cruz, A. J. G., Ramachandriya, K. D., Banat, I., & Wilkins, M. R. (2015). ETHANOL PRODUCTION FROM SUGARCANE BAGASSE USING SSF PROCESS AND THERMOTOLERANT YEAST. Transactions of the ASABE, 58(2), 193-200.