Ethanol production from cellulose at 45 degrees C using a hatch-fed system containing alginate-immobilized Kluyveromyces marxianus IMB3

N Barron, R Marchant, L McHale, Anthony McHale

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The thermotolerant yeast, Kluyveromyces marxianus IMB3, was grown in batch culture at 45 degrees C on cellulose-containing media, supplemented with exogenous cellulase activity. At various stages during fermentation, both substrate and enzyme were added in batch mode and fermentation was continued for 220 h. Ethanol production increased to 20 g/l at 200 h, representing 45% of the maximum theoretical yield. In subsequent experiments, the organism was immobilized in calcium alginate beads and these were used in a similar, batch-fed system at 45 degrees C. Again, fermentation was continued for 220 h and ethanol production increased to its maximum, of 28 g/l, within 100 h and this represented in excess of 60% of the maximum theoretical yield.
LanguageEnglish
Pages103-104
JournalWorld Journal of Microbiology and Biotechnology
Volume12
Issue number1
Publication statusPublished - Jan 1996

Fingerprint

Kluyveromyces marxianus
ethanol production
alginates
cellulose
fermentation
calcium alginate
endo-1,4-beta-glucanase
yeasts
organisms
enzymes

Cite this

@article{c75f2902e97349e78027013df9a4e959,
title = "Ethanol production from cellulose at 45 degrees C using a hatch-fed system containing alginate-immobilized Kluyveromyces marxianus IMB3",
abstract = "The thermotolerant yeast, Kluyveromyces marxianus IMB3, was grown in batch culture at 45 degrees C on cellulose-containing media, supplemented with exogenous cellulase activity. At various stages during fermentation, both substrate and enzyme were added in batch mode and fermentation was continued for 220 h. Ethanol production increased to 20 g/l at 200 h, representing 45{\%} of the maximum theoretical yield. In subsequent experiments, the organism was immobilized in calcium alginate beads and these were used in a similar, batch-fed system at 45 degrees C. Again, fermentation was continued for 220 h and ethanol production increased to its maximum, of 28 g/l, within 100 h and this represented in excess of 60{\%} of the maximum theoretical yield.",
author = "N Barron and R Marchant and L McHale and Anthony McHale",
year = "1996",
month = "1",
language = "English",
volume = "12",
pages = "103--104",
journal = "World Journal of Microbiology and Biotechnology",
issn = "0959-3993",
number = "1",

}

TY - JOUR

T1 - Ethanol production from cellulose at 45 degrees C using a hatch-fed system containing alginate-immobilized Kluyveromyces marxianus IMB3

AU - Barron, N

AU - Marchant, R

AU - McHale, L

AU - McHale, Anthony

PY - 1996/1

Y1 - 1996/1

N2 - The thermotolerant yeast, Kluyveromyces marxianus IMB3, was grown in batch culture at 45 degrees C on cellulose-containing media, supplemented with exogenous cellulase activity. At various stages during fermentation, both substrate and enzyme were added in batch mode and fermentation was continued for 220 h. Ethanol production increased to 20 g/l at 200 h, representing 45% of the maximum theoretical yield. In subsequent experiments, the organism was immobilized in calcium alginate beads and these were used in a similar, batch-fed system at 45 degrees C. Again, fermentation was continued for 220 h and ethanol production increased to its maximum, of 28 g/l, within 100 h and this represented in excess of 60% of the maximum theoretical yield.

AB - The thermotolerant yeast, Kluyveromyces marxianus IMB3, was grown in batch culture at 45 degrees C on cellulose-containing media, supplemented with exogenous cellulase activity. At various stages during fermentation, both substrate and enzyme were added in batch mode and fermentation was continued for 220 h. Ethanol production increased to 20 g/l at 200 h, representing 45% of the maximum theoretical yield. In subsequent experiments, the organism was immobilized in calcium alginate beads and these were used in a similar, batch-fed system at 45 degrees C. Again, fermentation was continued for 220 h and ethanol production increased to its maximum, of 28 g/l, within 100 h and this represented in excess of 60% of the maximum theoretical yield.

M3 - Article

VL - 12

SP - 103

EP - 104

JO - World Journal of Microbiology and Biotechnology

T2 - World Journal of Microbiology and Biotechnology

JF - World Journal of Microbiology and Biotechnology

SN - 0959-3993

IS - 1

ER -