Ethanol production at 45 degrees C using preparations of Kluyveromyces marxianus IMB3 immobilized in calcium alginate and kissiris

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Abstract

The thermotolerant yeast, K. marxianus IMB3, was grown in free and immobilized states in batch-fed culture at 45 degrees C and ethanol production was examined over a 61-day period. The organism was grown in the free state, in the free state with mineral kissiris, immobilized in calcium alginate and immobilized in calcium alginate together with kissiris. Initially, reactors were fed every two days with 10% (w/v) glucose-containing media and no significant difference in ethanol production was observed. In subsequent refeeding experiments, reactors were re-fed every two days with 15% (w/v) sucrose-containing media. Although overall ethanol concentrations decreased, production in the immobilized systems was higher. In the final stages fermentations were re-fed every 3 days and although overall ethanol production decreased further, production remained highest in the systems containing calcium alginate and kissiris.
LanguageEnglish
Pages275-277
JournalBioprocess Engineering
Volume15
Issue number5
Publication statusPublished - Oct 1996

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Kluyveromyces marxianus
calcium alginate
ethanol production
refeeding
ethanol
fermentation
sucrose
yeasts
minerals
glucose
organisms

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title = "Ethanol production at 45 degrees C using preparations of Kluyveromyces marxianus IMB3 immobilized in calcium alginate and kissiris",
abstract = "The thermotolerant yeast, K. marxianus IMB3, was grown in free and immobilized states in batch-fed culture at 45 degrees C and ethanol production was examined over a 61-day period. The organism was grown in the free state, in the free state with mineral kissiris, immobilized in calcium alginate and immobilized in calcium alginate together with kissiris. Initially, reactors were fed every two days with 10{\%} (w/v) glucose-containing media and no significant difference in ethanol production was observed. In subsequent refeeding experiments, reactors were re-fed every two days with 15{\%} (w/v) sucrose-containing media. Although overall ethanol concentrations decreased, production in the immobilized systems was higher. In the final stages fermentations were re-fed every 3 days and although overall ethanol production decreased further, production remained highest in the systems containing calcium alginate and kissiris.",
author = "G Love and {Singh - Nee Nigam}, Poonam and N Barron and D Singh and R Marchant and Anthony McHale",
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journal = "Bioprocess Engineering",
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TY - JOUR

T1 - Ethanol production at 45 degrees C using preparations of Kluyveromyces marxianus IMB3 immobilized in calcium alginate and kissiris

AU - Love, G

AU - Singh - Nee Nigam, Poonam

AU - Barron, N

AU - Singh, D

AU - Marchant, R

AU - McHale, Anthony

PY - 1996/10

Y1 - 1996/10

N2 - The thermotolerant yeast, K. marxianus IMB3, was grown in free and immobilized states in batch-fed culture at 45 degrees C and ethanol production was examined over a 61-day period. The organism was grown in the free state, in the free state with mineral kissiris, immobilized in calcium alginate and immobilized in calcium alginate together with kissiris. Initially, reactors were fed every two days with 10% (w/v) glucose-containing media and no significant difference in ethanol production was observed. In subsequent refeeding experiments, reactors were re-fed every two days with 15% (w/v) sucrose-containing media. Although overall ethanol concentrations decreased, production in the immobilized systems was higher. In the final stages fermentations were re-fed every 3 days and although overall ethanol production decreased further, production remained highest in the systems containing calcium alginate and kissiris.

AB - The thermotolerant yeast, K. marxianus IMB3, was grown in free and immobilized states in batch-fed culture at 45 degrees C and ethanol production was examined over a 61-day period. The organism was grown in the free state, in the free state with mineral kissiris, immobilized in calcium alginate and immobilized in calcium alginate together with kissiris. Initially, reactors were fed every two days with 10% (w/v) glucose-containing media and no significant difference in ethanol production was observed. In subsequent refeeding experiments, reactors were re-fed every two days with 15% (w/v) sucrose-containing media. Although overall ethanol concentrations decreased, production in the immobilized systems was higher. In the final stages fermentations were re-fed every 3 days and although overall ethanol production decreased further, production remained highest in the systems containing calcium alginate and kissiris.

M3 - Article

VL - 15

SP - 275

EP - 277

JO - Bioprocess Engineering

T2 - Bioprocess Engineering

JF - Bioprocess Engineering

SN - 0178-515X

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