Ethanol production at 45 degrees C by alginate-immobilized Kluyveromyces marxianus IMB3 during growth on lactose-containing media

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Abstract

The thermotolerant yeast strain Kluyveromyces marxianus IMB3 was immobilized in calcium alginate and this was used in batch-fed reactor systems to convert lactose (4 g/l) to ethanol. Production of ethanol by the free and immobilized biocatalyst in the presence and absence of Mn2+ was compared. In systems containing the free microorganism in the presence and absence of Mn2+, ethanol increased to a maxi mum of 8 g/l within 40 hours with no significant difference in production by both systems. Ethanol production by the immobilized system in the absence of Mn2+ increased to a maximum of 13 g/l within 40 hours and then decreased to 9 g/l within 80 hours. Ethanol production by the immobilized system in the presence of Mn2+ increased to 14 g/l within 60 hours and this decreased to 13 g/l at 80 hours. When all systems were re-fed at 80 hours, ethanol production by systems containing the free biocatalyst increased to a maximum of 3 g/l while the immobilized system in the presence of Mn2+ increased to a maximum of 12 g/l. Subsequent experiments involving re-feeding the system at shorter time intervals demonstrated that ethanol production by the immobilized system on lactose-containing media at 45 degrees C was far superior to ethanol production by the free biocatalyst.
LanguageEnglish
Pages101-104
JournalBioprocess Engineering
Volume16
Issue number2
Publication statusPublished - Jan 1997

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Kluyveromyces marxianus
ethanol production
alginates
lactose
ethanol
calcium alginate
Chrysanthemum morifolium
refeeding
yeasts
microorganisms
biocatalysts

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title = "Ethanol production at 45 degrees C by alginate-immobilized Kluyveromyces marxianus IMB3 during growth on lactose-containing media",
abstract = "The thermotolerant yeast strain Kluyveromyces marxianus IMB3 was immobilized in calcium alginate and this was used in batch-fed reactor systems to convert lactose (4 g/l) to ethanol. Production of ethanol by the free and immobilized biocatalyst in the presence and absence of Mn2+ was compared. In systems containing the free microorganism in the presence and absence of Mn2+, ethanol increased to a maxi mum of 8 g/l within 40 hours with no significant difference in production by both systems. Ethanol production by the immobilized system in the absence of Mn2+ increased to a maximum of 13 g/l within 40 hours and then decreased to 9 g/l within 80 hours. Ethanol production by the immobilized system in the presence of Mn2+ increased to 14 g/l within 60 hours and this decreased to 13 g/l at 80 hours. When all systems were re-fed at 80 hours, ethanol production by systems containing the free biocatalyst increased to a maximum of 3 g/l while the immobilized system in the presence of Mn2+ increased to a maximum of 12 g/l. Subsequent experiments involving re-feeding the system at shorter time intervals demonstrated that ethanol production by the immobilized system on lactose-containing media at 45 degrees C was far superior to ethanol production by the free biocatalyst.",
author = "D Brady and {Singh - Nee Nigam}, Poonam and Roger Marchant and Anthony McHale",
year = "1997",
month = "1",
language = "English",
volume = "16",
pages = "101--104",
journal = "Bioprocess Engineering",
issn = "0178-515X",
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T1 - Ethanol production at 45 degrees C by alginate-immobilized Kluyveromyces marxianus IMB3 during growth on lactose-containing media

AU - Brady, D

AU - Singh - Nee Nigam, Poonam

AU - Marchant, Roger

AU - McHale, Anthony

PY - 1997/1

Y1 - 1997/1

N2 - The thermotolerant yeast strain Kluyveromyces marxianus IMB3 was immobilized in calcium alginate and this was used in batch-fed reactor systems to convert lactose (4 g/l) to ethanol. Production of ethanol by the free and immobilized biocatalyst in the presence and absence of Mn2+ was compared. In systems containing the free microorganism in the presence and absence of Mn2+, ethanol increased to a maxi mum of 8 g/l within 40 hours with no significant difference in production by both systems. Ethanol production by the immobilized system in the absence of Mn2+ increased to a maximum of 13 g/l within 40 hours and then decreased to 9 g/l within 80 hours. Ethanol production by the immobilized system in the presence of Mn2+ increased to 14 g/l within 60 hours and this decreased to 13 g/l at 80 hours. When all systems were re-fed at 80 hours, ethanol production by systems containing the free biocatalyst increased to a maximum of 3 g/l while the immobilized system in the presence of Mn2+ increased to a maximum of 12 g/l. Subsequent experiments involving re-feeding the system at shorter time intervals demonstrated that ethanol production by the immobilized system on lactose-containing media at 45 degrees C was far superior to ethanol production by the free biocatalyst.

AB - The thermotolerant yeast strain Kluyveromyces marxianus IMB3 was immobilized in calcium alginate and this was used in batch-fed reactor systems to convert lactose (4 g/l) to ethanol. Production of ethanol by the free and immobilized biocatalyst in the presence and absence of Mn2+ was compared. In systems containing the free microorganism in the presence and absence of Mn2+, ethanol increased to a maxi mum of 8 g/l within 40 hours with no significant difference in production by both systems. Ethanol production by the immobilized system in the absence of Mn2+ increased to a maximum of 13 g/l within 40 hours and then decreased to 9 g/l within 80 hours. Ethanol production by the immobilized system in the presence of Mn2+ increased to 14 g/l within 60 hours and this decreased to 13 g/l at 80 hours. When all systems were re-fed at 80 hours, ethanol production by systems containing the free biocatalyst increased to a maximum of 3 g/l while the immobilized system in the presence of Mn2+ increased to a maximum of 12 g/l. Subsequent experiments involving re-feeding the system at shorter time intervals demonstrated that ethanol production by the immobilized system on lactose-containing media at 45 degrees C was far superior to ethanol production by the free biocatalyst.

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VL - 16

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EP - 104

JO - Bioprocess Engineering

T2 - Bioprocess Engineering

JF - Bioprocess Engineering

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