In comparing ethanol production by free and Ca-alginate immobilized cultures of the thermotolerant yeast, Kluyveromyces marxianus IMB3, on glucose-containing media at 45 degrees C, it was found that initial yields produced by the immobilized culture lagged behind those produced by cultures in free suspension. However, in subsequent batch-feed experiments it was demonstrated that the ethanol-producing ability of the immobilized preparation increased with successive feeds, while production by the free suspension reduced significantly.
NOLAN, AM., BARRON, N., BRADY, D., MCAREE, T., SMITH, D., MCHALE, L., & MCHALE, AP. (1994). ETHANOL-PRODUCTION AT 45-DEGREES BY AN ALGINATE-IMMOBILIZED THERMOTOLERANT STRAIN OF KLUYVEROMYCES-MARXIANUS FOLLOWING GROWTH ON GLUCOSE-CONTAINING MEDIA. Biotechnology Letters, 16(8), 849-852. https://doi.org/10.1007/BF00133966