The fast and direct measurement of leukocyte behaviour in microvessels in situ is possible by labelling them with fluorescent compounds and subsequently imaging them by confocal laser scanning microscopy. Using a high-speed single line scanning technique a time dependent trace of leukocytes in microvessels can be recorded (x-t scan). These x-t traces, which are orientated orthogonal to the blood flow, are characterized by distinct, but often noise-affected elliptical streaks, which carry information on speed and frequency of leukocytes crossing the scan line. Here we describe the combination of a confocal laser scanning system and an image analysis software package that allows rapid and reproducible estimation of leukocyte velocities in microvessels.
|Title of host publication||Unknown Host Publication|
|Publisher||IEEE Signal Processing Society|
|Number of pages||510|
|Publication status||Published - 2007|
|Event||2007 4TH IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING : MACRO TO NANO, VOLS 1-3 - |
Duration: 1 Jan 2007 → …
|Name||IEEE International Symposium on Biomedical Imaging|
|Other||2007 4TH IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING : MACRO TO NANO, VOLS 1-3|
|Period||1/01/07 → …|
Bigot, S., Lucas, L., Morrow, P., Mitchell, C., & Saetzler, K. (2007). Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow. In Unknown Host Publication (pp. 376-379). (IEEE International Symposium on Biomedical Imaging). IEEE Signal Processing Society.