Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow

Stephane Bigot, Luciano Lucas, Philip Morrow, Christopher Mitchell, Kurt Saetzler

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The fast and direct measurement of leukocyte behaviour in microvessels in situ is possible by labelling them with fluorescent compounds and subsequently imaging them by confocal laser scanning microscopy. Using a high-speed single line scanning technique a time dependent trace of leukocytes in microvessels can be recorded (x-t scan). These x-t traces, which are orientated orthogonal to the blood flow, are characterized by distinct, but often noise-affected elliptical streaks, which carry information on speed and frequency of leukocytes crossing the scan line. Here we describe the combination of a confocal laser scanning system and an image analysis software package that allows rapid and reproducible estimation of leukocyte velocities in microvessels.
LanguageEnglish
Title of host publicationUnknown Host Publication
Pages376-379
Number of pages510
Publication statusPublished - 2007
Event2007 4TH IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING : MACRO TO NANO, VOLS 1-3 -
Duration: 1 Jan 2007 → …

Publication series

NameIEEE International Symposium on Biomedical Imaging
PublisherISBI

Other

Other2007 4TH IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING : MACRO TO NANO, VOLS 1-3
Period1/01/07 → …

Fingerprint

laser
blood
image analysis
microscopy
software
speed
labelling
in situ

Cite this

Bigot, S., Lucas, L., Morrow, P., Mitchell, C., & Saetzler, K. (2007). Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow. In Unknown Host Publication (pp. 376-379). (IEEE International Symposium on Biomedical Imaging).
Bigot, Stephane ; Lucas, Luciano ; Morrow, Philip ; Mitchell, Christopher ; Saetzler, Kurt. / Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow. Unknown Host Publication. 2007. pp. 376-379 (IEEE International Symposium on Biomedical Imaging).
@inproceedings{df47f115e44d40ceb8e2080f22279691,
title = "Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow",
abstract = "The fast and direct measurement of leukocyte behaviour in microvessels in situ is possible by labelling them with fluorescent compounds and subsequently imaging them by confocal laser scanning microscopy. Using a high-speed single line scanning technique a time dependent trace of leukocytes in microvessels can be recorded (x-t scan). These x-t traces, which are orientated orthogonal to the blood flow, are characterized by distinct, but often noise-affected elliptical streaks, which carry information on speed and frequency of leukocytes crossing the scan line. Here we describe the combination of a confocal laser scanning system and an image analysis software package that allows rapid and reproducible estimation of leukocyte velocities in microvessels.",
author = "Stephane Bigot and Luciano Lucas and Philip Morrow and Christopher Mitchell and Kurt Saetzler",
note = "4th IEEE International Symposium on Biomedical Imaging - Macro to Nano, Arlington, VA, APR 12-15, 2007",
year = "2007",
language = "English",
series = "IEEE International Symposium on Biomedical Imaging",
publisher = "ISBI",
pages = "376--379",
booktitle = "Unknown Host Publication",

}

Bigot, S, Lucas, L, Morrow, P, Mitchell, C & Saetzler, K 2007, Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow. in Unknown Host Publication. IEEE International Symposium on Biomedical Imaging, pp. 376-379, 2007 4TH IEEE INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING : MACRO TO NANO, VOLS 1-3, 1/01/07.

Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow. / Bigot, Stephane; Lucas, Luciano; Morrow, Philip; Mitchell, Christopher; Saetzler, Kurt.

Unknown Host Publication. 2007. p. 376-379 (IEEE International Symposium on Biomedical Imaging).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow

AU - Bigot, Stephane

AU - Lucas, Luciano

AU - Morrow, Philip

AU - Mitchell, Christopher

AU - Saetzler, Kurt

N1 - 4th IEEE International Symposium on Biomedical Imaging - Macro to Nano, Arlington, VA, APR 12-15, 2007

PY - 2007

Y1 - 2007

N2 - The fast and direct measurement of leukocyte behaviour in microvessels in situ is possible by labelling them with fluorescent compounds and subsequently imaging them by confocal laser scanning microscopy. Using a high-speed single line scanning technique a time dependent trace of leukocytes in microvessels can be recorded (x-t scan). These x-t traces, which are orientated orthogonal to the blood flow, are characterized by distinct, but often noise-affected elliptical streaks, which carry information on speed and frequency of leukocytes crossing the scan line. Here we describe the combination of a confocal laser scanning system and an image analysis software package that allows rapid and reproducible estimation of leukocyte velocities in microvessels.

AB - The fast and direct measurement of leukocyte behaviour in microvessels in situ is possible by labelling them with fluorescent compounds and subsequently imaging them by confocal laser scanning microscopy. Using a high-speed single line scanning technique a time dependent trace of leukocytes in microvessels can be recorded (x-t scan). These x-t traces, which are orientated orthogonal to the blood flow, are characterized by distinct, but often noise-affected elliptical streaks, which carry information on speed and frequency of leukocytes crossing the scan line. Here we describe the combination of a confocal laser scanning system and an image analysis software package that allows rapid and reproducible estimation of leukocyte velocities in microvessels.

M3 - Conference contribution

T3 - IEEE International Symposium on Biomedical Imaging

SP - 376

EP - 379

BT - Unknown Host Publication

ER -

Bigot S, Lucas L, Morrow P, Mitchell C, Saetzler K. Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow. In Unknown Host Publication. 2007. p. 376-379. (IEEE International Symposium on Biomedical Imaging).