The fast and direct measurement of leukocyte behaviour in microvessels in situ is possible by labelling them with fluorescent compounds and subsequently imaging them by confocal laser scanning microscopy. Using a high-speed single line scanning technique a time dependent trace of leukocytes in microvessels can be recorded (x-t scan). These x-t traces, which are orientated orthogonal to the blood flow, are characterized by distinct, but often noise-affected elliptical streaks, which carry information on speed and frequency of leukocytes crossing the scan line. Here we describe the combination of a confocal laser scanning system and an image analysis software package that allows rapid and reproducible estimation of leukocyte velocities in microvessels.
|Title of host publication||Unknown Host Publication|
|Number of pages||4|
|Publication status||Published (in print/issue) - 2007|
|Event||Biomedical Imaging: Macro to Nano, 2007. 4rd IEEE International Symposium on - Washington|
Duration: 1 Jan 2007 → …
|Conference||Biomedical Imaging: Macro to Nano, 2007. 4rd IEEE International Symposium on|
|Period||1/01/07 → …|