Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow

S. Bigot, L. Lucas, PJ Morrow, Christopher Mitchell, Kurt Saetzler

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The fast and direct measurement of leukocyte behaviour in microvessels in situ is possible by labelling them with fluorescent compounds and subsequently imaging them by confocal laser scanning microscopy. Using a high-speed single line scanning technique a time dependent trace of leukocytes in microvessels can be recorded (x-t scan). These x-t traces, which are orientated orthogonal to the blood flow, are characterized by distinct, but often noise-affected elliptical streaks, which carry information on speed and frequency of leukocytes crossing the scan line. Here we describe the combination of a confocal laser scanning system and an image analysis software package that allows rapid and reproducible estimation of leukocyte velocities in microvessels.
LanguageEnglish
Title of host publicationUnknown Host Publication
Pages376-379
Number of pages4
DOIs
Publication statusPublished - 2007
EventBiomedical Imaging: Macro to Nano, 2007. 4rd IEEE International Symposium on - Washington
Duration: 1 Jan 2007 → …

Conference

ConferenceBiomedical Imaging: Macro to Nano, 2007. 4rd IEEE International Symposium on
Period1/01/07 → …

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laser
blood
image analysis
microscopy
software
speed
labelling
in situ

Cite this

@inproceedings{af3dc13390b14ef9953539bc0424bf76,
title = "Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow",
abstract = "The fast and direct measurement of leukocyte behaviour in microvessels in situ is possible by labelling them with fluorescent compounds and subsequently imaging them by confocal laser scanning microscopy. Using a high-speed single line scanning technique a time dependent trace of leukocytes in microvessels can be recorded (x-t scan). These x-t traces, which are orientated orthogonal to the blood flow, are characterized by distinct, but often noise-affected elliptical streaks, which carry information on speed and frequency of leukocytes crossing the scan line. Here we describe the combination of a confocal laser scanning system and an image analysis software package that allows rapid and reproducible estimation of leukocyte velocities in microvessels.",
author = "S. Bigot and L. Lucas and PJ Morrow and Christopher Mitchell and Kurt Saetzler",
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doi = "10.1109/ISBI.2007.356867",
language = "English",
pages = "376--379",
booktitle = "Unknown Host Publication",

}

Bigot, S, Lucas, L, Morrow, PJ, Mitchell, C & Saetzler, K 2007, Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow. in Unknown Host Publication. pp. 376-379, Biomedical Imaging: Macro to Nano, 2007. 4rd IEEE International Symposium on, 1/01/07. https://doi.org/10.1109/ISBI.2007.356867

Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow. / Bigot, S.; Lucas, L.; Morrow, PJ; Mitchell, Christopher; Saetzler, Kurt.

Unknown Host Publication. 2007. p. 376-379.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Estimating leukocyte velocities from high-speed 1D line scans oriented orthogonal to blood flow

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AU - Lucas, L.

AU - Morrow, PJ

AU - Mitchell, Christopher

AU - Saetzler, Kurt

PY - 2007

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N2 - The fast and direct measurement of leukocyte behaviour in microvessels in situ is possible by labelling them with fluorescent compounds and subsequently imaging them by confocal laser scanning microscopy. Using a high-speed single line scanning technique a time dependent trace of leukocytes in microvessels can be recorded (x-t scan). These x-t traces, which are orientated orthogonal to the blood flow, are characterized by distinct, but often noise-affected elliptical streaks, which carry information on speed and frequency of leukocytes crossing the scan line. Here we describe the combination of a confocal laser scanning system and an image analysis software package that allows rapid and reproducible estimation of leukocyte velocities in microvessels.

AB - The fast and direct measurement of leukocyte behaviour in microvessels in situ is possible by labelling them with fluorescent compounds and subsequently imaging them by confocal laser scanning microscopy. Using a high-speed single line scanning technique a time dependent trace of leukocytes in microvessels can be recorded (x-t scan). These x-t traces, which are orientated orthogonal to the blood flow, are characterized by distinct, but often noise-affected elliptical streaks, which carry information on speed and frequency of leukocytes crossing the scan line. Here we describe the combination of a confocal laser scanning system and an image analysis software package that allows rapid and reproducible estimation of leukocyte velocities in microvessels.

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DO - 10.1109/ISBI.2007.356867

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