Epidermal Growth factor potentiates the Transmitter-Induced Stimulation of C-AMP and Inositol Phosphates in Human Pigment Epithelial Cells in Culture

NP Liu, F FitzGibbon, M Nash, NN Osborne

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    Salbutamol, isoproterenol and dopamine stimulate C-AMP production in human retinal pigment epithelium (RPE) cells by activation of β2-type receptors. Epidermal growth factor (EGF) in contrast does not alter basal levels of C-AMP but elevates in an apparently dose-dependent manner the isoproterenol-induced stimulation of C-AMP. EGF also potentiates the forskolin-induced stimulation of C-AMP but has no effect on the elevation of C-AMP caused by NECA (5′-[N-ethyl]-carboxamido adenosine), an adenosine A2-receptor agonist. EGF, isoproterenol and NECA have no effect on basal levels of inositol phosphates (InsPs) in human RPE cells, but EGF specifically elevates the carbachol-induced stimulation of InsPs. The carbachol effect on InsPs is attenuated by the phorbol ester PMA (4β-phorbol 12 myrisate 13-acetate). PMA did not, however, affect the stimulation of C-AMP caused by isoproterenol. The interaction of EGF and C-AMP is further demonstrated in experiments where the incorporation of [3H]thymidine into RPE cells was studied, as an index for proliferation. EGF stimulates RPE cell proliferation while isoproterenol and dibutyryl C-AMP nullify the EGF effect. Dibutyryl C-AMP has a negative effect on RPE cell proliferation while isoproterenol is ineffective.The data presented here suggest that after stimulation of EGF receptors, tyrosine-kinase-activated products can influence secondary messenger products produced from activation of β2-type (linked with C-AMP formation) and muscarinic (linked with InsPs production) receptors in RPE cells. We could find no evidence of an interaction between receptors associated with C-AMP and InsPs/diacylglycerol production.
    LanguageEnglish
    Pages489-497
    JournalExperimental Eye Research
    Volume55
    Issue number3
    DOIs
    Publication statusPublished - Sep 1992

    Fingerprint

    Inositol Phosphates
    Adenosine Monophosphate
    Epidermal Growth Factor
    Cell Culture Techniques
    Epithelial Cells
    Retinal Pigment Epithelium
    Isoproterenol
    Carbachol
    Adenosine
    Acetates
    Adenosine A2 Receptor Agonists
    Cell Proliferation
    Albuterol
    Diglycerides
    Phorbol Esters
    Colforsin
    Epidermal Growth Factor Receptor
    Protein-Tyrosine Kinases
    Thymidine
    Cholinergic Agents

    Keywords

    • Epidermal growth factor
    • C-AMP
    • inositol phosphates
    • pigment epithelial cells

    Cite this

    @article{eb30e45aa93242edbe2901f7a85d24be,
    title = "Epidermal Growth factor potentiates the Transmitter-Induced Stimulation of C-AMP and Inositol Phosphates in Human Pigment Epithelial Cells in Culture",
    abstract = "Salbutamol, isoproterenol and dopamine stimulate C-AMP production in human retinal pigment epithelium (RPE) cells by activation of β2-type receptors. Epidermal growth factor (EGF) in contrast does not alter basal levels of C-AMP but elevates in an apparently dose-dependent manner the isoproterenol-induced stimulation of C-AMP. EGF also potentiates the forskolin-induced stimulation of C-AMP but has no effect on the elevation of C-AMP caused by NECA (5′-[N-ethyl]-carboxamido adenosine), an adenosine A2-receptor agonist. EGF, isoproterenol and NECA have no effect on basal levels of inositol phosphates (InsPs) in human RPE cells, but EGF specifically elevates the carbachol-induced stimulation of InsPs. The carbachol effect on InsPs is attenuated by the phorbol ester PMA (4β-phorbol 12 myrisate 13-acetate). PMA did not, however, affect the stimulation of C-AMP caused by isoproterenol. The interaction of EGF and C-AMP is further demonstrated in experiments where the incorporation of [3H]thymidine into RPE cells was studied, as an index for proliferation. EGF stimulates RPE cell proliferation while isoproterenol and dibutyryl C-AMP nullify the EGF effect. Dibutyryl C-AMP has a negative effect on RPE cell proliferation while isoproterenol is ineffective.The data presented here suggest that after stimulation of EGF receptors, tyrosine-kinase-activated products can influence secondary messenger products produced from activation of β2-type (linked with C-AMP formation) and muscarinic (linked with InsPs production) receptors in RPE cells. We could find no evidence of an interaction between receptors associated with C-AMP and InsPs/diacylglycerol production.",
    keywords = "Epidermal growth factor, C-AMP, inositol phosphates, pigment epithelial cells",
    author = "NP Liu and F FitzGibbon and M Nash and NN Osborne",
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    language = "English",
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    pages = "489--497",
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    Epidermal Growth factor potentiates the Transmitter-Induced Stimulation of C-AMP and Inositol Phosphates in Human Pigment Epithelial Cells in Culture. / Liu, NP; FitzGibbon, F; Nash, M; Osborne, NN.

    In: Experimental Eye Research, Vol. 55, No. 3, 09.1992, p. 489-497.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Epidermal Growth factor potentiates the Transmitter-Induced Stimulation of C-AMP and Inositol Phosphates in Human Pigment Epithelial Cells in Culture

    AU - Liu, NP

    AU - FitzGibbon, F

    AU - Nash, M

    AU - Osborne, NN

    PY - 1992/9

    Y1 - 1992/9

    N2 - Salbutamol, isoproterenol and dopamine stimulate C-AMP production in human retinal pigment epithelium (RPE) cells by activation of β2-type receptors. Epidermal growth factor (EGF) in contrast does not alter basal levels of C-AMP but elevates in an apparently dose-dependent manner the isoproterenol-induced stimulation of C-AMP. EGF also potentiates the forskolin-induced stimulation of C-AMP but has no effect on the elevation of C-AMP caused by NECA (5′-[N-ethyl]-carboxamido adenosine), an adenosine A2-receptor agonist. EGF, isoproterenol and NECA have no effect on basal levels of inositol phosphates (InsPs) in human RPE cells, but EGF specifically elevates the carbachol-induced stimulation of InsPs. The carbachol effect on InsPs is attenuated by the phorbol ester PMA (4β-phorbol 12 myrisate 13-acetate). PMA did not, however, affect the stimulation of C-AMP caused by isoproterenol. The interaction of EGF and C-AMP is further demonstrated in experiments where the incorporation of [3H]thymidine into RPE cells was studied, as an index for proliferation. EGF stimulates RPE cell proliferation while isoproterenol and dibutyryl C-AMP nullify the EGF effect. Dibutyryl C-AMP has a negative effect on RPE cell proliferation while isoproterenol is ineffective.The data presented here suggest that after stimulation of EGF receptors, tyrosine-kinase-activated products can influence secondary messenger products produced from activation of β2-type (linked with C-AMP formation) and muscarinic (linked with InsPs production) receptors in RPE cells. We could find no evidence of an interaction between receptors associated with C-AMP and InsPs/diacylglycerol production.

    AB - Salbutamol, isoproterenol and dopamine stimulate C-AMP production in human retinal pigment epithelium (RPE) cells by activation of β2-type receptors. Epidermal growth factor (EGF) in contrast does not alter basal levels of C-AMP but elevates in an apparently dose-dependent manner the isoproterenol-induced stimulation of C-AMP. EGF also potentiates the forskolin-induced stimulation of C-AMP but has no effect on the elevation of C-AMP caused by NECA (5′-[N-ethyl]-carboxamido adenosine), an adenosine A2-receptor agonist. EGF, isoproterenol and NECA have no effect on basal levels of inositol phosphates (InsPs) in human RPE cells, but EGF specifically elevates the carbachol-induced stimulation of InsPs. The carbachol effect on InsPs is attenuated by the phorbol ester PMA (4β-phorbol 12 myrisate 13-acetate). PMA did not, however, affect the stimulation of C-AMP caused by isoproterenol. The interaction of EGF and C-AMP is further demonstrated in experiments where the incorporation of [3H]thymidine into RPE cells was studied, as an index for proliferation. EGF stimulates RPE cell proliferation while isoproterenol and dibutyryl C-AMP nullify the EGF effect. Dibutyryl C-AMP has a negative effect on RPE cell proliferation while isoproterenol is ineffective.The data presented here suggest that after stimulation of EGF receptors, tyrosine-kinase-activated products can influence secondary messenger products produced from activation of β2-type (linked with C-AMP formation) and muscarinic (linked with InsPs production) receptors in RPE cells. We could find no evidence of an interaction between receptors associated with C-AMP and InsPs/diacylglycerol production.

    KW - Epidermal growth factor

    KW - C-AMP

    KW - inositol phosphates

    KW - pigment epithelial cells

    U2 - 10.1016/0014-4835(92)90122-9

    DO - 10.1016/0014-4835(92)90122-9

    M3 - Article

    VL - 55

    SP - 489

    EP - 497

    JO - Experimental Eye Research

    T2 - Experimental Eye Research

    JF - Experimental Eye Research

    SN - 0014-4835

    IS - 3

    ER -