Enzyme induction and cytotoxicity of phenethyl isothiocyanate and its glutathione conjugate towards breast cancer cells

Yui Sze Lam, Richard Owusu-Apenten

Research output: Contribution to journalArticle

Abstract

Chemoprevention using isothiocyanates is partly the result of the induction ofphase II enzymes for carcinogen detoxification from healthy cells. However,phase II enzyme activity can impair cancer therapeutic agents. The objective ofthis study was to assess phase II enzyme induction and cytotoxicity of phenethylisothiocyanate (PEITC) and glutathione conjugate with PEITC (GsPEITC) usingMCF-7 and MDA-MB-231 breast cancer cells. Changes of phase II enzymes,glutathione-S-transferase (GST) and NAD(P)H quinone reductase (QR), weremeasured by colorimetric procedures. Cell viability was determined using theMTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium- bromide) assay.From current results, 24hr exposure to 50 μM) of PEITC or GsPEITC increased phase IIenzyme activity by a maximum of 500-700-percent. To conclude, cytotoxic dosesof PEITC or GsPEITC possess phase II enzyme inducing activity in MCF-7 andMDA-MB-231 breast cancer cells.
LanguageEnglish
Pages63-73
JournalPure and Applied Chemical Sciences
Volume1
Issue number2
DOIs
Publication statusPublished - 2013

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Enzyme Induction
Glutathione
Breast Neoplasms
Enzymes
Isothiocyanates
NAD(P)H Dehydrogenase (Quinone)
Chemoprevention
Glutathione Transferase
Bromides
Carcinogens
NAD
Cell Survival
phenethyl isothiocyanate
Neoplasms
Therapeutics

Cite this

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title = "Enzyme induction and cytotoxicity of phenethyl isothiocyanate and its glutathione conjugate towards breast cancer cells",
abstract = "Chemoprevention using isothiocyanates is partly the result of the induction ofphase II enzymes for carcinogen detoxification from healthy cells. However,phase II enzyme activity can impair cancer therapeutic agents. The objective ofthis study was to assess phase II enzyme induction and cytotoxicity of phenethylisothiocyanate (PEITC) and glutathione conjugate with PEITC (GsPEITC) usingMCF-7 and MDA-MB-231 breast cancer cells. Changes of phase II enzymes,glutathione-S-transferase (GST) and NAD(P)H quinone reductase (QR), weremeasured by colorimetric procedures. Cell viability was determined using theMTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium- bromide) assay.From current results, 24hr exposure to 50 μM) of PEITC or GsPEITC increased phase IIenzyme activity by a maximum of 500-700-percent. To conclude, cytotoxic dosesof PEITC or GsPEITC possess phase II enzyme inducing activity in MCF-7 andMDA-MB-231 breast cancer cells.",
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Enzyme induction and cytotoxicity of phenethyl isothiocyanate and its glutathione conjugate towards breast cancer cells. / Lam, Yui Sze; Owusu-Apenten, Richard.

In: Pure and Applied Chemical Sciences, Vol. 1, No. 2, 2013, p. 63-73.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Enzyme induction and cytotoxicity of phenethyl isothiocyanate and its glutathione conjugate towards breast cancer cells

AU - Lam, Yui Sze

AU - Owusu-Apenten, Richard

PY - 2013

Y1 - 2013

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AB - Chemoprevention using isothiocyanates is partly the result of the induction ofphase II enzymes for carcinogen detoxification from healthy cells. However,phase II enzyme activity can impair cancer therapeutic agents. The objective ofthis study was to assess phase II enzyme induction and cytotoxicity of phenethylisothiocyanate (PEITC) and glutathione conjugate with PEITC (GsPEITC) usingMCF-7 and MDA-MB-231 breast cancer cells. Changes of phase II enzymes,glutathione-S-transferase (GST) and NAD(P)H quinone reductase (QR), weremeasured by colorimetric procedures. Cell viability was determined using theMTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium- bromide) assay.From current results, 24hr exposure to 50 μM) of PEITC or GsPEITC increased phase IIenzyme activity by a maximum of 500-700-percent. To conclude, cytotoxic dosesof PEITC or GsPEITC possess phase II enzyme inducing activity in MCF-7 andMDA-MB-231 breast cancer cells.

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