Environmental scanning electron microscopy as a novel tool to characterise real-time hydration of milk protein concentrates

Lucille Gallagher, Valeria Cenini, David McSweeney, Mark Auty, G McKerr, Noel McCarthy, Barry O'Hagan

Research output: Contribution to conferencePoster

Abstract

The physical state of milk protein concentrate (MPC) powder particles during hydration is not well defined. Nanoparticle size plays a crucial role in the structural, flow and compositional properties of milk powders especially so when determining their subsequent hydration profiles. Particle size is an extremely useful indicator of quality and performance, particularly in the reconstitution of milk powders. Mainstream research has investigated milk powder hydration using bulk density methods. Using advanced bioimaging technologies the real-time hydration of milk powders can be observed at the level of the individual particle. Currently there are no studies that have examined milk powder particles hydration environmental scanning electron microscopy (ESEM). The aim of this study was to characterise the real-time hydration of MPC particles using ESEM. Low-(MPC40 and 50), medium-(MPC65 and 75) and high-(MPC85) protein content MPC powders manufactured by Teagasc, Moorepark, Fermoy were stored at 54% and 88% relative humidity (RH) at 4 oC. The hydration of the MPC powders were investigated under ESEM to examine the effects of storage temperature and RH. MPC40 and MPC50 which were stored at 54% and 88% RH exhibited a weakening of the external framework leading to their subsequent collapse and in some cases full dissolution. MPC65 and MPC75 stored at 54% RH revealed large breaks on the external skin of powder particles and MPC85 displayed little dissolution. MPC65, MPC75 and MPC85 stored at 88% RH showed a graded disruption of the external skin of the powder particles. This study validates the use of ESEM as a novel tool to record dynamic in-situ hydration of milk powder particles

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Milk Proteins
dried milk
Hydration
Electron Scanning Microscopy
Powders
scanning electron microscopy
Scanning electron microscopy
powders
relative humidity
Humidity
Milk
Atmospheric humidity
skin (animal)
nanoparticles
Skin
Dissolution
milk protein concentrate
storage temperature
bulk density
particle size

Keywords

  • Microscopy

Cite this

Gallagher, L., Cenini, V., McSweeney, D., Auty, M., McKerr, G., McCarthy, N., & O'Hagan, B. (Accepted/In press). Environmental scanning electron microscopy as a novel tool to characterise real-time hydration of milk protein concentrates. Poster session presented at UCC-IFST Conference 2018, Cork, Ireland.
Gallagher, Lucille ; Cenini, Valeria ; McSweeney, David ; Auty, Mark ; McKerr, G ; McCarthy, Noel ; O'Hagan, Barry. / Environmental scanning electron microscopy as a novel tool to characterise real-time hydration of milk protein concentrates. Poster session presented at UCC-IFST Conference 2018, Cork, Ireland.
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abstract = "The physical state of milk protein concentrate (MPC) powder particles during hydration is not well defined. Nanoparticle size plays a crucial role in the structural, flow and compositional properties of milk powders especially so when determining their subsequent hydration profiles. Particle size is an extremely useful indicator of quality and performance, particularly in the reconstitution of milk powders. Mainstream research has investigated milk powder hydration using bulk density methods. Using advanced bioimaging technologies the real-time hydration of milk powders can be observed at the level of the individual particle. Currently there are no studies that have examined milk powder particles hydration environmental scanning electron microscopy (ESEM). The aim of this study was to characterise the real-time hydration of MPC particles using ESEM. Low-(MPC40 and 50), medium-(MPC65 and 75) and high-(MPC85) protein content MPC powders manufactured by Teagasc, Moorepark, Fermoy were stored at 54{\%} and 88{\%} relative humidity (RH) at 4 oC. The hydration of the MPC powders were investigated under ESEM to examine the effects of storage temperature and RH. MPC40 and MPC50 which were stored at 54{\%} and 88{\%} RH exhibited a weakening of the external framework leading to their subsequent collapse and in some cases full dissolution. MPC65 and MPC75 stored at 54{\%} RH revealed large breaks on the external skin of powder particles and MPC85 displayed little dissolution. MPC65, MPC75 and MPC85 stored at 88{\%} RH showed a graded disruption of the external skin of the powder particles. This study validates the use of ESEM as a novel tool to record dynamic in-situ hydration of milk powder particles",
keywords = "Microscopy",
author = "Lucille Gallagher and Valeria Cenini and David McSweeney and Mark Auty and G McKerr and Noel McCarthy and Barry O'Hagan",
year = "2018",
month = "11",
day = "17",
language = "English",
note = "UCC-IFST Conference 2018 : 47th Annual Food Science and Technology Conference ; Conference date: 06-12-2018 Through 07-12-2018",
url = "https://www.ucc.ie/en/fns/47thannualfoodresearchconference/",

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Gallagher, L, Cenini, V, McSweeney, D, Auty, M, McKerr, G, McCarthy, N & O'Hagan, B 2018, 'Environmental scanning electron microscopy as a novel tool to characterise real-time hydration of milk protein concentrates' UCC-IFST Conference 2018, Cork, Ireland, 6/12/18 - 7/12/18, .

Environmental scanning electron microscopy as a novel tool to characterise real-time hydration of milk protein concentrates. / Gallagher, Lucille; Cenini, Valeria; McSweeney, David; Auty, Mark; McKerr, G; McCarthy, Noel; O'Hagan, Barry.

2018. Poster session presented at UCC-IFST Conference 2018, Cork, Ireland.

Research output: Contribution to conferencePoster

TY - CONF

T1 - Environmental scanning electron microscopy as a novel tool to characterise real-time hydration of milk protein concentrates

AU - Gallagher, Lucille

AU - Cenini, Valeria

AU - McSweeney, David

AU - Auty, Mark

AU - McKerr, G

AU - McCarthy, Noel

AU - O'Hagan, Barry

PY - 2018/11/17

Y1 - 2018/11/17

N2 - The physical state of milk protein concentrate (MPC) powder particles during hydration is not well defined. Nanoparticle size plays a crucial role in the structural, flow and compositional properties of milk powders especially so when determining their subsequent hydration profiles. Particle size is an extremely useful indicator of quality and performance, particularly in the reconstitution of milk powders. Mainstream research has investigated milk powder hydration using bulk density methods. Using advanced bioimaging technologies the real-time hydration of milk powders can be observed at the level of the individual particle. Currently there are no studies that have examined milk powder particles hydration environmental scanning electron microscopy (ESEM). The aim of this study was to characterise the real-time hydration of MPC particles using ESEM. Low-(MPC40 and 50), medium-(MPC65 and 75) and high-(MPC85) protein content MPC powders manufactured by Teagasc, Moorepark, Fermoy were stored at 54% and 88% relative humidity (RH) at 4 oC. The hydration of the MPC powders were investigated under ESEM to examine the effects of storage temperature and RH. MPC40 and MPC50 which were stored at 54% and 88% RH exhibited a weakening of the external framework leading to their subsequent collapse and in some cases full dissolution. MPC65 and MPC75 stored at 54% RH revealed large breaks on the external skin of powder particles and MPC85 displayed little dissolution. MPC65, MPC75 and MPC85 stored at 88% RH showed a graded disruption of the external skin of the powder particles. This study validates the use of ESEM as a novel tool to record dynamic in-situ hydration of milk powder particles

AB - The physical state of milk protein concentrate (MPC) powder particles during hydration is not well defined. Nanoparticle size plays a crucial role in the structural, flow and compositional properties of milk powders especially so when determining their subsequent hydration profiles. Particle size is an extremely useful indicator of quality and performance, particularly in the reconstitution of milk powders. Mainstream research has investigated milk powder hydration using bulk density methods. Using advanced bioimaging technologies the real-time hydration of milk powders can be observed at the level of the individual particle. Currently there are no studies that have examined milk powder particles hydration environmental scanning electron microscopy (ESEM). The aim of this study was to characterise the real-time hydration of MPC particles using ESEM. Low-(MPC40 and 50), medium-(MPC65 and 75) and high-(MPC85) protein content MPC powders manufactured by Teagasc, Moorepark, Fermoy were stored at 54% and 88% relative humidity (RH) at 4 oC. The hydration of the MPC powders were investigated under ESEM to examine the effects of storage temperature and RH. MPC40 and MPC50 which were stored at 54% and 88% RH exhibited a weakening of the external framework leading to their subsequent collapse and in some cases full dissolution. MPC65 and MPC75 stored at 54% RH revealed large breaks on the external skin of powder particles and MPC85 displayed little dissolution. MPC65, MPC75 and MPC85 stored at 88% RH showed a graded disruption of the external skin of the powder particles. This study validates the use of ESEM as a novel tool to record dynamic in-situ hydration of milk powder particles

KW - Microscopy

M3 - Poster

ER -

Gallagher L, Cenini V, McSweeney D, Auty M, McKerr G, McCarthy N et al. Environmental scanning electron microscopy as a novel tool to characterise real-time hydration of milk protein concentrates. 2018. Poster session presented at UCC-IFST Conference 2018, Cork, Ireland.