An enthalpimetric determination of urea using immobilized urease in a flow system is described. Sensitivity of analysis is related to the mole reaction enthalpy (dHR). This is estimated as (61 ±3 kJ mol−1 and 20 ±1 kJ mol−1 in sodium phosphate and tris-HCl buffer respectively. Factors affecting the minimum amount of urea detected, linearity range and rate of analysis are discussed.
title = "Enthalpimetric flow analysis of urea using immobilized urease",
abstract = "An enthalpimetric determination of urea using immobilized urease in a flow system is described. Sensitivity of analysis is related to the mole reaction enthalpy (dHR). This is estimated as (61 ±3 kJ mol−1 and 20 ±1 kJ mol−1 in sodium phosphate and tris-HCl buffer respectively. Factors affecting the minimum amount of urea detected, linearity range and rate of analysis are discussed.",
author = "Owusu, {Richard K} and Trewhella, {Martin J} and Arthur Finch",
Research output: Contribution to journal › Article
TY - JOUR
T1 - Enthalpimetric flow analysis of urea using immobilized urease
AU - Owusu, Richard K
AU - Trewhella, Martin J
AU - Finch, Arthur
PY - 1987/2/15
Y1 - 1987/2/15
N2 - An enthalpimetric determination of urea using immobilized urease in a flow system is described. Sensitivity of analysis is related to the mole reaction enthalpy (dHR). This is estimated as (61 ±3 kJ mol−1 and 20 ±1 kJ mol−1 in sodium phosphate and tris-HCl buffer respectively. Factors affecting the minimum amount of urea detected, linearity range and rate of analysis are discussed.
AB - An enthalpimetric determination of urea using immobilized urease in a flow system is described. Sensitivity of analysis is related to the mole reaction enthalpy (dHR). This is estimated as (61 ±3 kJ mol−1 and 20 ±1 kJ mol−1 in sodium phosphate and tris-HCl buffer respectively. Factors affecting the minimum amount of urea detected, linearity range and rate of analysis are discussed.