Enthalpimetric flow analysis of urea using immobilized urease

Richard K Owusu, Martin J Trewhella, Arthur Finch

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

An enthalpimetric determination of urea using immobilized urease in a flow system is described. Sensitivity of analysis is related to the mole reaction enthalpy (dHR). This is estimated as (61 ±3 kJ mol−1 and 20 ±1 kJ mol−1 in sodium phosphate and tris-HCl buffer respectively. Factors affecting the minimum amount of urea detected, linearity range and rate of analysis are discussed.
LanguageEnglish
Pages1-7
JournalThermochimica Acta
Volume111
Publication statusPublished - 15 Feb 1987

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Urease
ureas
Urea
linearity
Enthalpy
phosphates
Buffers
Phosphates
buffers
enthalpy
Sodium
sodium
sensitivity
sodium phosphate

Cite this

Owusu, R. K., Trewhella, M. J., & Finch, A. (1987). Enthalpimetric flow analysis of urea using immobilized urease. Thermochimica Acta, 111, 1-7.
Owusu, Richard K ; Trewhella, Martin J ; Finch, Arthur. / Enthalpimetric flow analysis of urea using immobilized urease. In: Thermochimica Acta. 1987 ; Vol. 111. pp. 1-7.
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Owusu, RK, Trewhella, MJ & Finch, A 1987, 'Enthalpimetric flow analysis of urea using immobilized urease', Thermochimica Acta, vol. 111, pp. 1-7.

Enthalpimetric flow analysis of urea using immobilized urease. / Owusu, Richard K; Trewhella, Martin J; Finch, Arthur.

In: Thermochimica Acta, Vol. 111, 15.02.1987, p. 1-7.

Research output: Contribution to journalArticle

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AU - Owusu, Richard K

AU - Trewhella, Martin J

AU - Finch, Arthur

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N2 - An enthalpimetric determination of urea using immobilized urease in a flow system is described. Sensitivity of analysis is related to the mole reaction enthalpy (dHR). This is estimated as (61 ±3 kJ mol−1 and 20 ±1 kJ mol−1 in sodium phosphate and tris-HCl buffer respectively. Factors affecting the minimum amount of urea detected, linearity range and rate of analysis are discussed.

AB - An enthalpimetric determination of urea using immobilized urease in a flow system is described. Sensitivity of analysis is related to the mole reaction enthalpy (dHR). This is estimated as (61 ±3 kJ mol−1 and 20 ±1 kJ mol−1 in sodium phosphate and tris-HCl buffer respectively. Factors affecting the minimum amount of urea detected, linearity range and rate of analysis are discussed.

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Owusu RK, Trewhella MJ, Finch A. Enthalpimetric flow analysis of urea using immobilized urease. Thermochimica Acta. 1987 Feb 15;111:1-7.