An enthalpimetric determination of urea using immobilized urease in a flow system is described. Sensitivity of analysis is related to the mole reaction enthalpy (dHR). This is estimated as (61 ±3 kJ mol−1 and 20 ±1 kJ mol−1 in sodium phosphate and tris-HCl buffer respectively. Factors affecting the minimum amount of urea detected, linearity range and rate of analysis are discussed.
|Publication status||Published - 15 Feb 1987|
Owusu, R. K., Trewhella, M. J., & Finch, A. (1987). Enthalpimetric flow analysis of urea using immobilized urease. Thermochimica Acta, 111, 1-7. http://www.sciencedirect.com/science/article/pii/0040603187880280