Enterolactone restricts the proliferation of the LNCaP human prostate cancer cell line in vitro

Mark J. McCann, Chris Gill, Trevor Linton, D. Berrar, Hugh McGlynn, Ian R. Rowland

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Ecological data suggest a long-term diet high in plant material rich in biologically active compounds, such as the lignans, can significantly influence the development of prostate cancer over the lifetime of an individual. The capacity of a pure mammalian lignan, enterolactone (ENL), to influence the proliferation of the LNCaP human prostate cancer cell line was investigated as a function of cell density, metabolic activity, expression and secretion of prostate specific antigen (PSA), cell cycle profile, and the expression of genes involved in development and progression of prostate cancer. Treatment with a subcytotoxic concentration of ENL (60 mu M for 72 h) was found to reduce: cell density (57.5%, SD 7.23, p < 0.001), metabolic activity (55%, SD 0.03, p < 0.001), secretion of PSA (48.50% SD 4.74, p = 0.05) and induce apoptosis (8.33-fold SD 0.04, p = 0.001) compared to untreated cells. Cotreatment with 10 mu M etoposide was found to increase apoptosis by 50.17% (SD 0.02, p < 0.001). Additionally, several key genes (e.g. MCMs, survivin and CDKs) were beneficially regulated by ENL treatment (p < 0.05). The data suggest that the antiproliferative activity of ENL is a consequence of altered expression of cell cycle associated genes and provides novel molecular evidence for the antiproliferative properties of a pure lignan in prostate cancer.
LanguageEnglish
Pages567-580
JournalMolecular Nutrition and Food Research
Volume52
Issue number5
DOIs
Publication statusPublished - May 2008

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Lignans
Prostatic Neoplasms
Cell Line
Prostate-Specific Antigen
Cell Count
Apoptosis
cdc Genes
Etoposide
Transcriptome
Cell Cycle
Diet
2,3-bis(3'-hydroxybenzyl)butyrolactone
In Vitro Techniques
Genes

Cite this

McCann, Mark J. ; Gill, Chris ; Linton, Trevor ; Berrar, D. ; McGlynn, Hugh ; Rowland, Ian R. / Enterolactone restricts the proliferation of the LNCaP human prostate cancer cell line in vitro. In: Molecular Nutrition and Food Research. 2008 ; Vol. 52, No. 5. pp. 567-580.
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abstract = "Ecological data suggest a long-term diet high in plant material rich in biologically active compounds, such as the lignans, can significantly influence the development of prostate cancer over the lifetime of an individual. The capacity of a pure mammalian lignan, enterolactone (ENL), to influence the proliferation of the LNCaP human prostate cancer cell line was investigated as a function of cell density, metabolic activity, expression and secretion of prostate specific antigen (PSA), cell cycle profile, and the expression of genes involved in development and progression of prostate cancer. Treatment with a subcytotoxic concentration of ENL (60 mu M for 72 h) was found to reduce: cell density (57.5{\%}, SD 7.23, p < 0.001), metabolic activity (55{\%}, SD 0.03, p < 0.001), secretion of PSA (48.50{\%} SD 4.74, p = 0.05) and induce apoptosis (8.33-fold SD 0.04, p = 0.001) compared to untreated cells. Cotreatment with 10 mu M etoposide was found to increase apoptosis by 50.17{\%} (SD 0.02, p < 0.001). Additionally, several key genes (e.g. MCMs, survivin and CDKs) were beneficially regulated by ENL treatment (p < 0.05). The data suggest that the antiproliferative activity of ENL is a consequence of altered expression of cell cycle associated genes and provides novel molecular evidence for the antiproliferative properties of a pure lignan in prostate cancer.",
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Enterolactone restricts the proliferation of the LNCaP human prostate cancer cell line in vitro. / McCann, Mark J.; Gill, Chris; Linton, Trevor; Berrar, D.; McGlynn, Hugh; Rowland, Ian R.

In: Molecular Nutrition and Food Research, Vol. 52, No. 5, 05.2008, p. 567-580.

Research output: Contribution to journalArticle

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AU - Gill, Chris

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AU - Rowland, Ian R.

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AB - Ecological data suggest a long-term diet high in plant material rich in biologically active compounds, such as the lignans, can significantly influence the development of prostate cancer over the lifetime of an individual. The capacity of a pure mammalian lignan, enterolactone (ENL), to influence the proliferation of the LNCaP human prostate cancer cell line was investigated as a function of cell density, metabolic activity, expression and secretion of prostate specific antigen (PSA), cell cycle profile, and the expression of genes involved in development and progression of prostate cancer. Treatment with a subcytotoxic concentration of ENL (60 mu M for 72 h) was found to reduce: cell density (57.5%, SD 7.23, p < 0.001), metabolic activity (55%, SD 0.03, p < 0.001), secretion of PSA (48.50% SD 4.74, p = 0.05) and induce apoptosis (8.33-fold SD 0.04, p = 0.001) compared to untreated cells. Cotreatment with 10 mu M etoposide was found to increase apoptosis by 50.17% (SD 0.02, p < 0.001). Additionally, several key genes (e.g. MCMs, survivin and CDKs) were beneficially regulated by ENL treatment (p < 0.05). The data suggest that the antiproliferative activity of ENL is a consequence of altered expression of cell cycle associated genes and provides novel molecular evidence for the antiproliferative properties of a pure lignan in prostate cancer.

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