Enhancing ultrasound-mediated cell membrane permeabilisation (sonoporation) using a high frequency pulse regime and implications for ultrasound-aided cancer chemotherapy

Ying Suet Li, Cherith N. Reid, Anthony McHale

Research output: Contribution to journalArticle

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Abstract

Delivering ultrasound to HeLa cells at 1 MHz using a high frequency pulse regime (40 kHz) and at a maximum energy density of 270 Jcm(-2) resulted in significant cell membrane permeabilisation. Using FITC-dextran as a fluorogenic marker.. optimally up to 64% of treated populations were permeabilised with cell viability remaining above 80%. Although cell membrane permeabilisation was observed in the presence of the microbubble-based ultrasound contrast agent, SonoVue (R), cell viability was severely compromised. Using the high frequency pulse regime in the absence of microbubbles, the LD50 of the cancer chemotherapeutic agent, camptothecin, was reduced from 58 to 18 nM. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
LanguageEnglish
Pages156-162
JournalCancer Letters
Volume266
Issue number2
DOIs
Publication statusPublished - Aug 2008

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Microbubbles
Cell Survival
Cell Membrane
Drug Therapy
Camptothecin
Lethal Dose 50
HeLa Cells
Contrast Media
Neoplasms
Population
contrast agent BR1
fluorescein isothiocyanate dextran

Cite this

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Enhancing ultrasound-mediated cell membrane permeabilisation (sonoporation) using a high frequency pulse regime and implications for ultrasound-aided cancer chemotherapy. / Li, Ying Suet; Reid, Cherith N.; McHale, Anthony.

Vol. 266, No. 2, 08.2008, p. 156-162.

Research output: Contribution to journalArticle

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