Abstract
| Language | English |
|---|---|
| Pages | 209-216 |
| Journal | British Journal of Pharmaceutical Research |
| Volume | 5 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2015 |
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Enhanced Growth-inhibitory Effect of Microemulsified Curcumin Formulation in Human Prostate Cancer LNCaP Cells. / Dubey, Vaibhav; Apenten, Richard KO.
In: British Journal of Pharmaceutical Research, Vol. 5, No. 3, 2015, p. 209-216.Research output: Contribution to journal › Article
TY - JOUR
T1 - Enhanced Growth-inhibitory Effect of Microemulsified Curcumin Formulation in Human Prostate Cancer LNCaP Cells
AU - Dubey, Vaibhav
AU - Apenten, Richard KO
PY - 2015
Y1 - 2015
N2 - Aim: To assess the effect of curcumin microemulsified with non-ionic surfactant surfynol 465 W or dispersed using edible oils on prostate LNCaP cancer cell viability and glutathione status.Methodology: LNCaP cells were treated for 72-144 hr with curcumin dissolved with fish or corn oil and microemulsified using non-ionic surfactant surfynol 465 W; alternatively LNCaP cells were treated with curcumin directly dispersed in fish or corn oil (0-50 μM) for 24 -72-144 hr. Cell viability was determined using resazurin (Vision blueTM) fluorescence assay. Glutathione status was determined by monochlorobimane (MCB) assay.Results: Treatment with 0-34 μM of microemulsified curcumin produced moderate cytotoxic effect on LNCaP cells, no 50% reduction of cell viability was observed graphically. However, when LNCaP cells were treated with curcumin dispersed with corn oil the concentration or 50% reduction of cell viability (IC50) was 12-45 μM. Similarly for cells treated with curcumin dispersed with fish oil, the IC50 was between 20-40 μM. Cytotoxic doses of curcumin dispersed with corn or fish oil increased GST status in cells by 272-656% (p =
AB - Aim: To assess the effect of curcumin microemulsified with non-ionic surfactant surfynol 465 W or dispersed using edible oils on prostate LNCaP cancer cell viability and glutathione status.Methodology: LNCaP cells were treated for 72-144 hr with curcumin dissolved with fish or corn oil and microemulsified using non-ionic surfactant surfynol 465 W; alternatively LNCaP cells were treated with curcumin directly dispersed in fish or corn oil (0-50 μM) for 24 -72-144 hr. Cell viability was determined using resazurin (Vision blueTM) fluorescence assay. Glutathione status was determined by monochlorobimane (MCB) assay.Results: Treatment with 0-34 μM of microemulsified curcumin produced moderate cytotoxic effect on LNCaP cells, no 50% reduction of cell viability was observed graphically. However, when LNCaP cells were treated with curcumin dispersed with corn oil the concentration or 50% reduction of cell viability (IC50) was 12-45 μM. Similarly for cells treated with curcumin dispersed with fish oil, the IC50 was between 20-40 μM. Cytotoxic doses of curcumin dispersed with corn or fish oil increased GST status in cells by 272-656% (p =
U2 - 10.9734/BJPR/2015/14475
DO - 10.9734/BJPR/2015/14475
M3 - Article
VL - 5
SP - 209
EP - 216
JO - British Journal of Pharmaceutical Research
T2 - British Journal of Pharmaceutical Research
JF - British Journal of Pharmaceutical Research
SN - 2231-2919
IS - 3
ER -