Abstract
Approximately 4.2 million people are suffering from corneal opacities according to the world health organisation (WHO) as of 2019. There is a shortage of allogeneic tissue donors, with only 79,641 people receiving corneal transplants in the United States in 2021.
Current alternatives to allogenic transplantation include the Boston type-1 keratoprosthesis. Suitable bioengineered solutions must display sufficient mechanical durability and processability and possess essential biocompatibility characteristics associated with the cornea's extracellular matrix (ECM).
Poly(L-lactide-co-ε-caprolactone) (PLCL) was electrospun to produce a suitable corneal scaffold to support the culture of human corneal epithelial (HCE-T) cells
This work investigated the physical, chemical and biological characteristics of electrospun PLCL scaffolds and how the rate of cellular attachment, viability and migration can be increased by using dielectric barrier discharge (DBD) plasma. Methods utilised included scanning electron microscopy, tensile testing, XPS analysis and FTIR, crystal violet, and MTT assays.
DBD plasma processing showed no significant difference in the tensile properties or in the FTIR spectra while XPS analysis revealed a significant increase (P
DBD plasma processing was shown to cause a significant increase in cellular attachment and viability and increase HCE-T wound healing time over 24hrs while evoking no significant changes to the physical characteristics of the PLCL scaffold.
Current alternatives to allogenic transplantation include the Boston type-1 keratoprosthesis. Suitable bioengineered solutions must display sufficient mechanical durability and processability and possess essential biocompatibility characteristics associated with the cornea's extracellular matrix (ECM).
Poly(L-lactide-co-ε-caprolactone) (PLCL) was electrospun to produce a suitable corneal scaffold to support the culture of human corneal epithelial (HCE-T) cells
This work investigated the physical, chemical and biological characteristics of electrospun PLCL scaffolds and how the rate of cellular attachment, viability and migration can be increased by using dielectric barrier discharge (DBD) plasma. Methods utilised included scanning electron microscopy, tensile testing, XPS analysis and FTIR, crystal violet, and MTT assays.
DBD plasma processing showed no significant difference in the tensile properties or in the FTIR spectra while XPS analysis revealed a significant increase (P
DBD plasma processing was shown to cause a significant increase in cellular attachment and viability and increase HCE-T wound healing time over 24hrs while evoking no significant changes to the physical characteristics of the PLCL scaffold.
| Original language | English |
|---|---|
| Pages | 76 |
| Number of pages | 1 |
| DOIs | |
| Publication status | Published online - 10 Apr 2023 |
| Event | 2023 TERMIS – AMERICAS Conference & Exhibition Boston Marriott Copley Place April 11–14, 2023 - Boston , Boston, United States Duration: 11 Apr 2023 → 14 Apr 2023 https://doi.org/10.1089/ten.tea.2023.29041.abstracts |
Conference
| Conference | 2023 TERMIS – AMERICAS Conference & Exhibition Boston Marriott Copley Place April 11–14, 2023 |
|---|---|
| Abbreviated title | TERMIS |
| Country/Territory | United States |
| City | Boston |
| Period | 11/04/23 → 14/04/23 |
| Internet address |
Keywords
- corneal epithelium
- wound healing
- electrospinning
