Enhanced cell viability and cell adhesion using low conductivity medium for negative dielectrophoretic cell patterning

Srinivasu Valagerahally Puttaswamy, Shilpa Sivashankar, Rong‐Jhe Chen, Chung‐Kuang Chin, Hwan‐You Chang, Cheng Hsien Liu

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Negative dielectrophoretic (n-DEP) cell manipulation is an efficient way to pattern human liver cells on micro-electrode arrays. Maintaining cell viability is an important objective for this approach. This study investigates the effect of low conductivity medium and the optimally designed microchip on cell viability and cell adhesion. To explore the influence of conductivity on cell viability and cell adhesion, we have used earlier reported dielectrophoresis (DEP) buffer with a conductivity of 10.2 mS/m and three formulated media with conductivity of 9.02 (M1), 8.14 (M2), 9.55 (M3) mS/m. The earlier reported isotonic sucrose/dextrose buffer (DEP buffer) used for DEP manipulation has the drawback of poor cell adhesion and cell viability. A microchip prototype with well-defined positioning of titanium electrode arrays was designed and fabricated on a glass substrate. The gap between the radial electrodes was accurately determined to achieve good cell patterning performance. Parameters such as dimension of positioning electrode, amplitude, and frequency of voltage signal were investigated to optimize the performance of the microchip.
Original languageEnglish
Pages (from-to)1005-1015
Number of pages11
JournalBiotechnology journal
Issue number10
Publication statusPublished - 7 Oct 2010



  • Cell adhesion
  • Cell patterning
  • Negative dielectrophoresis

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