Endocytic Structures and Synaptic Vesicle Recycling at a Central Synapse in Awake Rats.

Christoph Körber, Heinz Horstmann, Kurt Saetzler, Thomas Kuner

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    The synaptic vesicle (SV) cycle has been studied extensively in cultured cells and slice preparations, but not much is known about the roles and relative contributions of endocytic pathways and mechanisms of SV recycling in vivo, under physiological patterns of activity. We employed horseradish peroxidase (HRP) as an in vivo marker of endocytosis at the calyx of Held synapse in the awake rat. Ex vivo serial section scanning electron microscopy and 3D reconstructions revealed two categories of labelled structures: HRP-filled SVs and large cisternal endosomes. Inhibition of adaptor protein complexes 1 and 3 (AP-1, AP-3) by in vivo application of Brefeldin A (BFA) disrupted endosomal SV budding while SV recycling via clathrin-mediated endocytosis (CME) remained unaffected. In conclusion, our study establishes cisternal endosomes as an intermediate of the SV cycle and reveals CME and endosomal budding as the predominant mechanisms of SV recycling in a tonically active central synapse in vivo.
    LanguageEnglish
    Pages1601�1611
    JournalTraffic
    Volume13
    DOIs
    Publication statusPublished - Sep 2012

    Fingerprint

    Transport Vesicles
    Synaptic Vesicles
    Synapses
    Endocytosis
    Clathrin
    Endosomes
    Horseradish Peroxidase
    Adaptor Protein Complex 1
    Adaptor Protein Complex 3
    Brefeldin A
    Transcription Factor AP-1
    Electron Scanning Microscopy
    Cultured Cells

    Cite this

    Körber, C., Horstmann, H., Saetzler, K., & Kuner, T. (2012). Endocytic Structures and Synaptic Vesicle Recycling at a Central Synapse in Awake Rats. Traffic, 13, 1601â��1611. https://doi.org/10.1111/tra.12007
    Körber, Christoph ; Horstmann, Heinz ; Saetzler, Kurt ; Kuner, Thomas. / Endocytic Structures and Synaptic Vesicle Recycling at a Central Synapse in Awake Rats. In: Traffic. 2012 ; Vol. 13. pp. 1601â��1611.
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    Körber, C, Horstmann, H, Saetzler, K & Kuner, T 2012, 'Endocytic Structures and Synaptic Vesicle Recycling at a Central Synapse in Awake Rats.', Traffic, vol. 13, pp. 1601â��1611. https://doi.org/10.1111/tra.12007

    Endocytic Structures and Synaptic Vesicle Recycling at a Central Synapse in Awake Rats. / Körber, Christoph; Horstmann, Heinz; Saetzler, Kurt; Kuner, Thomas.

    In: Traffic, Vol. 13, 09.2012, p. 1601�1611.

    Research output: Contribution to journalArticle

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