ENCAPSULATION OF THE THROMBOLYTIC ENZYME, BRINASE, IN PHOTOSENSITIZED ERYTHROCYTES - A NOVEL THROMBOLYTIC SYSTEM BASED ON PHOTODYNAMIC ACTIVATION

G FLYNN, TJ HACKETT, L MCHALE, AP MCHALE

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

In order to circumvent many of the problems associated with the systemic administration of agents used in thrombolytic therapy, it was decided to investigate the possibility of using erythrocytes as carriers and delivery vehicles for these agents. The enzyme brinase, a fibrinolytic enzyme produced by Aspergillus oryzae, was loaded into rabbit erythrocytes using electroporation. The loading index for this enzyme was found to be 60% and incorporation appeared to be relatively stable over a period of 4 h. In order to facilitate the predetermined release of the loaded component from the erythrocytes, they were photosensitized using haematoporphyrin derivative (HPD) and release was demonstrated within 5 min of photoactivation. Inclusion of the loaded, photosensitized system into clotting blood and subsequent exposure to light demonstrated almost complete lysis of the clot. We believe that this system exhibits potential for use in thrombolytic therapy.
LanguageEnglish
Pages193-196
JournalJOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Volume26
Issue number2
DOIs
Publication statusPublished - Nov 1994

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encapsulation
erythrocytes
enzymes
blood coagulation
therapeutics
Aspergillus oryzae
electroporation
chemical derivatives
rabbits

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@article{9e3091e9b9d9451d8699cd8b9a6420f2,
title = "ENCAPSULATION OF THE THROMBOLYTIC ENZYME, BRINASE, IN PHOTOSENSITIZED ERYTHROCYTES - A NOVEL THROMBOLYTIC SYSTEM BASED ON PHOTODYNAMIC ACTIVATION",
abstract = "In order to circumvent many of the problems associated with the systemic administration of agents used in thrombolytic therapy, it was decided to investigate the possibility of using erythrocytes as carriers and delivery vehicles for these agents. The enzyme brinase, a fibrinolytic enzyme produced by Aspergillus oryzae, was loaded into rabbit erythrocytes using electroporation. The loading index for this enzyme was found to be 60{\%} and incorporation appeared to be relatively stable over a period of 4 h. In order to facilitate the predetermined release of the loaded component from the erythrocytes, they were photosensitized using haematoporphyrin derivative (HPD) and release was demonstrated within 5 min of photoactivation. Inclusion of the loaded, photosensitized system into clotting blood and subsequent exposure to light demonstrated almost complete lysis of the clot. We believe that this system exhibits potential for use in thrombolytic therapy.",
author = "G FLYNN and TJ HACKETT and L MCHALE and AP MCHALE",
year = "1994",
month = "11",
doi = "10.1016/1011-1344(94)07037-7",
language = "English",
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pages = "193--196",
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}

ENCAPSULATION OF THE THROMBOLYTIC ENZYME, BRINASE, IN PHOTOSENSITIZED ERYTHROCYTES - A NOVEL THROMBOLYTIC SYSTEM BASED ON PHOTODYNAMIC ACTIVATION. / FLYNN, G; HACKETT, TJ; MCHALE, L; MCHALE, AP.

Vol. 26, No. 2, 11.1994, p. 193-196.

Research output: Contribution to journalArticle

TY - JOUR

T1 - ENCAPSULATION OF THE THROMBOLYTIC ENZYME, BRINASE, IN PHOTOSENSITIZED ERYTHROCYTES - A NOVEL THROMBOLYTIC SYSTEM BASED ON PHOTODYNAMIC ACTIVATION

AU - FLYNN, G

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AU - MCHALE, L

AU - MCHALE, AP

PY - 1994/11

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AB - In order to circumvent many of the problems associated with the systemic administration of agents used in thrombolytic therapy, it was decided to investigate the possibility of using erythrocytes as carriers and delivery vehicles for these agents. The enzyme brinase, a fibrinolytic enzyme produced by Aspergillus oryzae, was loaded into rabbit erythrocytes using electroporation. The loading index for this enzyme was found to be 60% and incorporation appeared to be relatively stable over a period of 4 h. In order to facilitate the predetermined release of the loaded component from the erythrocytes, they were photosensitized using haematoporphyrin derivative (HPD) and release was demonstrated within 5 min of photoactivation. Inclusion of the loaded, photosensitized system into clotting blood and subsequent exposure to light demonstrated almost complete lysis of the clot. We believe that this system exhibits potential for use in thrombolytic therapy.

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