Emulated circulatory system integrated with lobule-mimetic liver to enhance the liver function

Shilpa Sivashankar; Srinivasu Valagerahally Puttaswamy; Tz-shuian Dai; Ciou-wun Lin; Shih-mo Yang; Hwan-you Chang; Cheng Hsien Liu

doi:10.1109/MEMSYS.2012.6170193

Emulated circulatory system integrated with lobule-mimetic liver to enhance the liver function

Shilpa Sivashankar, Srinivasu Valagerahally Puttaswamy, Tz-shuian Dai, Ciou-wun Lin, Shih-mo Yang, Hwan-you Chang, Cheng Hsien Liu

Research output: Contribution to conference › Paper › peer-review

3 Citations (Scopus)

Abstract

In the proposed paper, we report dielectrophoresis (DEP) liver lab chip integrated with Poly ethylene glycol diacrylate (PEGDA) based blood vessel within the microfluidic system for reconstructing the engineered liver tissue with the feature of liver function enhancement. The titanium (Ti) electrodes are well designed to provide positive dielectrophoresis (pDEP) and negative dielectrophoresis (nDEP) force to pattern HepG2 and 3T3 fibroblast cells. To mimic the blood flow direction of the liver lobule in vivo, we have integrated the reconstructed liver lobule with hydrogel encapsulated blood vessel. As smooth muscle cells and endothelial cells are the main components of blood vessel, we have utilized these cells and encapsulated within PEGDA hydrogel to mimic blood vessels. When assayed for urea concentration in cultured medium for five time points, the trend illustrated the enhanced metabolism of hepatocytes via blood vessel integrated lobule-mimetic pattern.

Original language	English
Pages	815-818
DOIs	https://doi.org/10.1109/MEMSYS.2012.6170193
Publication status	Published (in print/issue) - Jan 2012
Event	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems (MEMS) - Paris, France Duration: 29 Jan 2012 → 2 Feb 2012

Conference

Conference	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems (MEMS)
Period	29/01/12 → 2/02/12

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10.1109/MEMSYS.2012.6170193

http://ieeexplore.ieee.org/document/6170193/

Cite this

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title = "Emulated circulatory system integrated with lobule-mimetic liver to enhance the liver function",

abstract = "In the proposed paper, we report dielectrophoresis (DEP) liver lab chip integrated with Poly ethylene glycol diacrylate (PEGDA) based blood vessel within the microfluidic system for reconstructing the engineered liver tissue with the feature of liver function enhancement. The titanium (Ti) electrodes are well designed to provide positive dielectrophoresis (pDEP) and negative dielectrophoresis (nDEP) force to pattern HepG2 and 3T3 fibroblast cells. To mimic the blood flow direction of the liver lobule in vivo, we have integrated the reconstructed liver lobule with hydrogel encapsulated blood vessel. As smooth muscle cells and endothelial cells are the main components of blood vessel, we have utilized these cells and encapsulated within PEGDA hydrogel to mimic blood vessels. When assayed for urea concentration in cultured medium for five time points, the trend illustrated the enhanced metabolism of hepatocytes via blood vessel integrated lobule-mimetic pattern.",

author = "Shilpa Sivashankar and Puttaswamy, {Srinivasu Valagerahally} and Tz-shuian Dai and Ciou-wun Lin and Shih-mo Yang and Hwan-you Chang and Liu, {Cheng Hsien}",

year = "2012",

month = jan,

doi = "10.1109/MEMSYS.2012.6170193",

language = "English",

pages = "815--818",

note = "2012 IEEE 25th International Conference on Micro Electro Mechanical Systems (MEMS) ; Conference date: 29-01-2012 Through 02-02-2012",

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T1 - Emulated circulatory system integrated with lobule-mimetic liver to enhance the liver function

AU - Sivashankar, Shilpa

AU - Puttaswamy, Srinivasu Valagerahally

AU - Dai, Tz-shuian

AU - Lin, Ciou-wun

AU - Yang, Shih-mo

AU - Chang, Hwan-you

AU - Liu, Cheng Hsien

PY - 2012/1

Y1 - 2012/1

N2 - In the proposed paper, we report dielectrophoresis (DEP) liver lab chip integrated with Poly ethylene glycol diacrylate (PEGDA) based blood vessel within the microfluidic system for reconstructing the engineered liver tissue with the feature of liver function enhancement. The titanium (Ti) electrodes are well designed to provide positive dielectrophoresis (pDEP) and negative dielectrophoresis (nDEP) force to pattern HepG2 and 3T3 fibroblast cells. To mimic the blood flow direction of the liver lobule in vivo, we have integrated the reconstructed liver lobule with hydrogel encapsulated blood vessel. As smooth muscle cells and endothelial cells are the main components of blood vessel, we have utilized these cells and encapsulated within PEGDA hydrogel to mimic blood vessels. When assayed for urea concentration in cultured medium for five time points, the trend illustrated the enhanced metabolism of hepatocytes via blood vessel integrated lobule-mimetic pattern.

AB - In the proposed paper, we report dielectrophoresis (DEP) liver lab chip integrated with Poly ethylene glycol diacrylate (PEGDA) based blood vessel within the microfluidic system for reconstructing the engineered liver tissue with the feature of liver function enhancement. The titanium (Ti) electrodes are well designed to provide positive dielectrophoresis (pDEP) and negative dielectrophoresis (nDEP) force to pattern HepG2 and 3T3 fibroblast cells. To mimic the blood flow direction of the liver lobule in vivo, we have integrated the reconstructed liver lobule with hydrogel encapsulated blood vessel. As smooth muscle cells and endothelial cells are the main components of blood vessel, we have utilized these cells and encapsulated within PEGDA hydrogel to mimic blood vessels. When assayed for urea concentration in cultured medium for five time points, the trend illustrated the enhanced metabolism of hepatocytes via blood vessel integrated lobule-mimetic pattern.

U2 - 10.1109/MEMSYS.2012.6170193

DO - 10.1109/MEMSYS.2012.6170193

M3 - Paper

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T2 - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems (MEMS)

Y2 - 29 January 2012 through 2 February 2012

ER -

Emulated circulatory system integrated with lobule-mimetic liver to enhance the liver function

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