Elevated levels of oxidative DNA damage in lymphocytes from patients with Alzheimer's disease

M Morocz, J Kalman, A Juhasz, I Sinko, AP McGlynn, Stephen Downes, Z Janka, I Rasko

Research output: Contribution to journalArticlepeer-review

112 Citations (Scopus)


Previous studies have provided evidence of the involvement of oxidative damage in the pathogenesis of Alzheimer's disease (AD). Although the role of oxidative stress in the aetiology of the disease is still not clear, the detection of an increased damage status in the cells of patients could have important therapeutic implications. The level of oxidative damage and repair capacity in peripheral lymphocytes of AD patients and of age-matched controls was determined by the Comet assay applied to freshly isolated blood samples with oxidative lesion-specific DNA repair endonucleases. This is less prone to errors arising from oxidative artifacts than chemical analytical methods; and is therefore a relatively reliable, as well as rapid method for assay of oxidative DNA damage in cells. Statistically significant elevations (P < 0.05) of oxidized purines were observed in nuclear DNA of peripheral lymphocytes from AD patients, compared. to age matched control subjects, both at basal level and after oxidative stress induced by H2O2. AD patients also showed a diminished repair of H2O2 -induced oxidized purines. 
Original languageEnglish
Pages (from-to)47-53
Number of pages7
JournalNeurobiology of Aging
Issue number1
Early online date17 Dec 2001
Publication statusPublished (in print/issue) - 31 Jan 2002


  • Alzheimer's disease
  • Lymphocytes
  • Oxidative stress
  • Comet assay
  • DNA-repair


Dive into the research topics of 'Elevated levels of oxidative DNA damage in lymphocytes from patients with Alzheimer's disease'. Together they form a unique fingerprint.

Cite this