Electrochemically driven derivatisation-detection of cysteine

PC White, NS Lawrence, YC Tsai, J Davis, RG Compton

    Research output: Contribution to journalArticle

    36 Citations (Scopus)

    Abstract

    The electrochemical oxidation of catechol to ortho quinone at a glassy carbon electrode in the presence of cysteine is shown to lead to the deposition of a quinone-cysteine adduct at the electrode surface. Square wave voltammetry is shown to enable voltammetric resolution of unreacted orthoquinone and the accumulated adduct species such that quantification of both species is possible over a range of pH 2-pH 6. The reaction is shown to be selective towards cysteine with little interference from cystine, homocysteine or glutathione. The foundations of a detection protocol are reported with the sensitivity shown to be capable of attenuation through the facile manipulation of the number of electro-initiated derivatisation scans. The application of six consecutive derivatisation scans is shown to provide a linear range of 2-20 muM cysteine that is well within the region required for physiological monitoring.
    LanguageEnglish
    Pages87-91
    JournalMicrochimica Acta
    Volume137
    Issue number1-2
    Publication statusPublished - 2001

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    Cysteine
    Electrodes
    Cystine
    Electrochemical oxidation
    Glassy carbon
    Homocysteine
    Voltammetry
    Glutathione
    Monitoring
    benzoquinone

    Cite this

    White, PC., Lawrence, NS., Tsai, YC., Davis, J., & Compton, RG. (2001). Electrochemically driven derivatisation-detection of cysteine. Microchimica Acta, 137(1-2), 87-91.
    White, PC ; Lawrence, NS ; Tsai, YC ; Davis, J ; Compton, RG. / Electrochemically driven derivatisation-detection of cysteine. In: Microchimica Acta. 2001 ; Vol. 137, No. 1-2. pp. 87-91.
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    White, PC, Lawrence, NS, Tsai, YC, Davis, J & Compton, RG 2001, 'Electrochemically driven derivatisation-detection of cysteine', Microchimica Acta, vol. 137, no. 1-2, pp. 87-91.

    Electrochemically driven derivatisation-detection of cysteine. / White, PC; Lawrence, NS; Tsai, YC; Davis, J; Compton, RG.

    In: Microchimica Acta, Vol. 137, No. 1-2, 2001, p. 87-91.

    Research output: Contribution to journalArticle

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    AU - Lawrence, NS

    AU - Tsai, YC

    AU - Davis, J

    AU - Compton, RG

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    AB - The electrochemical oxidation of catechol to ortho quinone at a glassy carbon electrode in the presence of cysteine is shown to lead to the deposition of a quinone-cysteine adduct at the electrode surface. Square wave voltammetry is shown to enable voltammetric resolution of unreacted orthoquinone and the accumulated adduct species such that quantification of both species is possible over a range of pH 2-pH 6. The reaction is shown to be selective towards cysteine with little interference from cystine, homocysteine or glutathione. The foundations of a detection protocol are reported with the sensitivity shown to be capable of attenuation through the facile manipulation of the number of electro-initiated derivatisation scans. The application of six consecutive derivatisation scans is shown to provide a linear range of 2-20 muM cysteine that is well within the region required for physiological monitoring.

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    White PC, Lawrence NS, Tsai YC, Davis J, Compton RG. Electrochemically driven derivatisation-detection of cysteine. Microchimica Acta. 2001;137(1-2):87-91.