Electrochemically driven derivatisation-detection of cysteine

PC White, NS Lawrence, YC Tsai, J Davis, RG Compton

    Research output: Contribution to journalArticle

    38 Citations (Scopus)

    Abstract

    The electrochemical oxidation of catechol to ortho quinone at a glassy carbon electrode in the presence of cysteine is shown to lead to the deposition of a quinone-cysteine adduct at the electrode surface. Square wave voltammetry is shown to enable voltammetric resolution of unreacted orthoquinone and the accumulated adduct species such that quantification of both species is possible over a range of pH 2-pH 6. The reaction is shown to be selective towards cysteine with little interference from cystine, homocysteine or glutathione. The foundations of a detection protocol are reported with the sensitivity shown to be capable of attenuation through the facile manipulation of the number of electro-initiated derivatisation scans. The application of six consecutive derivatisation scans is shown to provide a linear range of 2-20 muM cysteine that is well within the region required for physiological monitoring.
    Original languageEnglish
    Pages (from-to)87-91
    JournalMicrochimica Acta
    Volume137
    Issue number1-2
    Publication statusPublished - 2001

    Fingerprint Dive into the research topics of 'Electrochemically driven derivatisation-detection of cysteine'. Together they form a unique fingerprint.

  • Cite this

    White, PC., Lawrence, NS., Tsai, YC., Davis, J., & Compton, RG. (2001). Electrochemically driven derivatisation-detection of cysteine. Microchimica Acta, 137(1-2), 87-91.