Electrochemical detection of thiols in biological media

NS Lawrence, J Davis, RG Compton

    Research output: Contribution to journalArticle

    110 Citations (Scopus)

    Abstract

    The utilisation of catechol as an electrochemical indicator fur the presence of sulphydryl thiols (RSH) has been investigated. The electrochemical oxidation of the catechol within tissue culture media was examined with the influence exerted on the redox chemistry by cysteine evaluated in terms of the development of an analytical protocol. The electro-generation of o-quinone was found to be followed by a 1,4-addition reaction with available cysteine such that an increase in the current, attributed to the re-oxidation of the thiol-catechol adduct, could be exploited as means of quantifying the concentration of the thiol. The selectivity of the reaction has been assessed with no interference from lysine, tyrosine, methionine or cystine. Other amino acids possessing sulphydryl thiol functionalities (homocysteine and glutathione) were, however, found to react through a similar route to that observed with cysteine. (C) 2001 Elsevier Science B.V. All rights reserved.
    LanguageEnglish
    Pages1089-1094
    JournalTalanta
    Volume53
    Issue number5
    Publication statusPublished - Jan 2001

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    Sulfhydryl Compounds
    Cysteine
    Tissue culture
    Addition reactions
    Cystine
    Electrochemical oxidation
    Homocysteine
    Methionine
    Lysine
    Glutathione
    Tyrosine
    Culture Media
    Amino Acids
    Oxidation
    catechol

    Cite this

    Lawrence, NS., Davis, J., & Compton, RG. (2001). Electrochemical detection of thiols in biological media. Talanta, 53(5), 1089-1094.
    Lawrence, NS ; Davis, J ; Compton, RG. / Electrochemical detection of thiols in biological media. In: Talanta. 2001 ; Vol. 53, No. 5. pp. 1089-1094.
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    Lawrence, NS, Davis, J & Compton, RG 2001, 'Electrochemical detection of thiols in biological media', Talanta, vol. 53, no. 5, pp. 1089-1094.

    Electrochemical detection of thiols in biological media. / Lawrence, NS; Davis, J; Compton, RG.

    In: Talanta, Vol. 53, No. 5, 01.2001, p. 1089-1094.

    Research output: Contribution to journalArticle

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    AB - The utilisation of catechol as an electrochemical indicator fur the presence of sulphydryl thiols (RSH) has been investigated. The electrochemical oxidation of the catechol within tissue culture media was examined with the influence exerted on the redox chemistry by cysteine evaluated in terms of the development of an analytical protocol. The electro-generation of o-quinone was found to be followed by a 1,4-addition reaction with available cysteine such that an increase in the current, attributed to the re-oxidation of the thiol-catechol adduct, could be exploited as means of quantifying the concentration of the thiol. The selectivity of the reaction has been assessed with no interference from lysine, tyrosine, methionine or cystine. Other amino acids possessing sulphydryl thiol functionalities (homocysteine and glutathione) were, however, found to react through a similar route to that observed with cysteine. (C) 2001 Elsevier Science B.V. All rights reserved.

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    Lawrence NS, Davis J, Compton RG. Electrochemical detection of thiols in biological media. Talanta. 2001 Jan;53(5):1089-1094.