Electrochemical detection of thiols in biological media

NS Lawrence, J Davis, RG Compton

    Research output: Contribution to journalArticle

    115 Citations (Scopus)

    Abstract

    The utilisation of catechol as an electrochemical indicator fur the presence of sulphydryl thiols (RSH) has been investigated. The electrochemical oxidation of the catechol within tissue culture media was examined with the influence exerted on the redox chemistry by cysteine evaluated in terms of the development of an analytical protocol. The electro-generation of o-quinone was found to be followed by a 1,4-addition reaction with available cysteine such that an increase in the current, attributed to the re-oxidation of the thiol-catechol adduct, could be exploited as means of quantifying the concentration of the thiol. The selectivity of the reaction has been assessed with no interference from lysine, tyrosine, methionine or cystine. Other amino acids possessing sulphydryl thiol functionalities (homocysteine and glutathione) were, however, found to react through a similar route to that observed with cysteine. (C) 2001 Elsevier Science B.V. All rights reserved.
    Original languageEnglish
    Pages (from-to)1089-1094
    JournalTalanta
    Volume53
    Issue number5
    Publication statusPublished - Jan 2001

    Fingerprint Dive into the research topics of 'Electrochemical detection of thiols in biological media'. Together they form a unique fingerprint.

    Cite this