Electroanalytical exploitation of quinone-thiol interactions: application to the selective determination of cysteine

G Hignett, S Threlfell, AJ Wain, NS Lawrence, SJ Wilkins, J Davis, RG Compton, MF Cardosi

    Research output: Contribution to journalArticle

    61 Citations (Scopus)

    Abstract

    Square wave voltammetry was applied to the detection of cysteine through the use of an indirect assay that exploits the reaction of the thiol with a quinone indicator. Voltammetric discrimination between unreacted quinone and the corresponding quinone-cysteine adduct is possible with clear resolution of the latter peak providing a linear response from 5 to 47 muM. The selectivity of the approach was assessed with no interference from cystine, lysine, paracetamol or 4-aminophenol. The response recorded in the presence of a massive excess of ascorbic acid was also investigated and the integrity of the approach confirmed. The effects of other sulfhydryl thiols, homocysteine and glutathione, were also assessed and found to present no appreciable change in the voltammetric profile. The practical utility of the approach was investigated through examining the response to cysteine in urine.
    LanguageEnglish
    Pages353-357
    JournalANALYST
    Volume126
    Issue number3
    Publication statusPublished - 2001

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    Sulfhydryl Compounds
    Cysteine
    Cystine
    Homocysteine
    Voltammetry
    Acetaminophen
    Ascorbic Acid
    Lysine
    Glutathione
    Assays
    benzoquinone

    Cite this

    Hignett, G., Threlfell, S., Wain, AJ., Lawrence, NS., Wilkins, SJ., Davis, J., ... Cardosi, MF. (2001). Electroanalytical exploitation of quinone-thiol interactions: application to the selective determination of cysteine. ANALYST, 126(3), 353-357.
    Hignett, G ; Threlfell, S ; Wain, AJ ; Lawrence, NS ; Wilkins, SJ ; Davis, J ; Compton, RG ; Cardosi, MF. / Electroanalytical exploitation of quinone-thiol interactions: application to the selective determination of cysteine. In: ANALYST. 2001 ; Vol. 126, No. 3. pp. 353-357.
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    abstract = "Square wave voltammetry was applied to the detection of cysteine through the use of an indirect assay that exploits the reaction of the thiol with a quinone indicator. Voltammetric discrimination between unreacted quinone and the corresponding quinone-cysteine adduct is possible with clear resolution of the latter peak providing a linear response from 5 to 47 muM. The selectivity of the approach was assessed with no interference from cystine, lysine, paracetamol or 4-aminophenol. The response recorded in the presence of a massive excess of ascorbic acid was also investigated and the integrity of the approach confirmed. The effects of other sulfhydryl thiols, homocysteine and glutathione, were also assessed and found to present no appreciable change in the voltammetric profile. The practical utility of the approach was investigated through examining the response to cysteine in urine.",
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    Hignett, G, Threlfell, S, Wain, AJ, Lawrence, NS, Wilkins, SJ, Davis, J, Compton, RG & Cardosi, MF 2001, 'Electroanalytical exploitation of quinone-thiol interactions: application to the selective determination of cysteine', ANALYST, vol. 126, no. 3, pp. 353-357.

    Electroanalytical exploitation of quinone-thiol interactions: application to the selective determination of cysteine. / Hignett, G; Threlfell, S; Wain, AJ; Lawrence, NS; Wilkins, SJ; Davis, J; Compton, RG; Cardosi, MF.

    In: ANALYST, Vol. 126, No. 3, 2001, p. 353-357.

    Research output: Contribution to journalArticle

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    AU - Davis, J

    AU - Compton, RG

    AU - Cardosi, MF

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    AB - Square wave voltammetry was applied to the detection of cysteine through the use of an indirect assay that exploits the reaction of the thiol with a quinone indicator. Voltammetric discrimination between unreacted quinone and the corresponding quinone-cysteine adduct is possible with clear resolution of the latter peak providing a linear response from 5 to 47 muM. The selectivity of the approach was assessed with no interference from cystine, lysine, paracetamol or 4-aminophenol. The response recorded in the presence of a massive excess of ascorbic acid was also investigated and the integrity of the approach confirmed. The effects of other sulfhydryl thiols, homocysteine and glutathione, were also assessed and found to present no appreciable change in the voltammetric profile. The practical utility of the approach was investigated through examining the response to cysteine in urine.

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    Hignett G, Threlfell S, Wain AJ, Lawrence NS, Wilkins SJ, Davis J et al. Electroanalytical exploitation of quinone-thiol interactions: application to the selective determination of cysteine. ANALYST. 2001;126(3):353-357.