Electric field-enhanced activation of hematoporphyrin derivative: Effects on a human tumour cell line

T Ward, D Mooney, G Flynn, AP McHale

Research output: Contribution to journalArticle

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Abstract

In a recent report we described the effects of combined electroactivation and photoactivation of hematoporphyrin derivative (HPD) on human erythrocytes and established that activation-induced cell lysis was more pronounced when both modes of activation were sequentially applied to the system. Here we demonstrate that electric field-induced activation of HPD-treated HeLa cells results in cell death, This effect is shown to be dependant on both electric field strength and on HPD concentration. In addition, we demonstrate that exposure of HPD-treated cells to shea and intense electric pulses prior to photoactivation, results in increased cell mortality. The results confirm our earlier suggestion that HPD may be activated in the presence of an applied electric field, The results further suggest that activation of photosensitizers using combined exposure to electric fields and light may play an important role in increasing the efficiency of photodynamic therapy (PDT) in the treatment of cancer. (C) 1997 Elsevier Science Ireland Ltd.
LanguageEnglish
Pages145-151
JournalCANCER LETTERS
Volume113
Issue number1-2
DOIs
Publication statusPublished - Feb 1997

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Hematoporphyrin Derivative
Tumor Cell Line
Photosensitizing Agents
Photochemotherapy
HeLa Cells
Cell Death
Erythrocytes
Light
Mortality
Neoplasms

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abstract = "In a recent report we described the effects of combined electroactivation and photoactivation of hematoporphyrin derivative (HPD) on human erythrocytes and established that activation-induced cell lysis was more pronounced when both modes of activation were sequentially applied to the system. Here we demonstrate that electric field-induced activation of HPD-treated HeLa cells results in cell death, This effect is shown to be dependant on both electric field strength and on HPD concentration. In addition, we demonstrate that exposure of HPD-treated cells to shea and intense electric pulses prior to photoactivation, results in increased cell mortality. The results confirm our earlier suggestion that HPD may be activated in the presence of an applied electric field, The results further suggest that activation of photosensitizers using combined exposure to electric fields and light may play an important role in increasing the efficiency of photodynamic therapy (PDT) in the treatment of cancer. (C) 1997 Elsevier Science Ireland Ltd.",
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Electric field-enhanced activation of hematoporphyrin derivative: Effects on a human tumour cell line. / Ward, T; Mooney, D; Flynn, G; McHale, AP.

Vol. 113, No. 1-2, 02.1997, p. 145-151.

Research output: Contribution to journalArticle

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T1 - Electric field-enhanced activation of hematoporphyrin derivative: Effects on a human tumour cell line

AU - Ward, T

AU - Mooney, D

AU - Flynn, G

AU - McHale, AP

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AB - In a recent report we described the effects of combined electroactivation and photoactivation of hematoporphyrin derivative (HPD) on human erythrocytes and established that activation-induced cell lysis was more pronounced when both modes of activation were sequentially applied to the system. Here we demonstrate that electric field-induced activation of HPD-treated HeLa cells results in cell death, This effect is shown to be dependant on both electric field strength and on HPD concentration. In addition, we demonstrate that exposure of HPD-treated cells to shea and intense electric pulses prior to photoactivation, results in increased cell mortality. The results confirm our earlier suggestion that HPD may be activated in the presence of an applied electric field, The results further suggest that activation of photosensitizers using combined exposure to electric fields and light may play an important role in increasing the efficiency of photodynamic therapy (PDT) in the treatment of cancer. (C) 1997 Elsevier Science Ireland Ltd.

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