Efficacy of the solar water disinfection method in turbid waters experimentally contaminated withCryptosporidium parvumoocysts under real field conditions

H. Gómez-Couso, M. Fontán-Saínz, C. Sichel, P Fernandez Ibanez, E. Ares-Mazás

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

bjective To investigate the efficacy of the solar water disinfection (SODIS) method for inactivating Cryptosporidium parvum oocysts in turbid waters using 1.5 l polyethylene terephthalate (PET) bottles under natural sunlight.methods All experiments were performed at the Plataforma Solar de Almerı ́a, located in the Tabernas Desert (Southern Spain) in July and October 2007. Turbid water samples [5, 100 and 300 nephelometric turbidity units (NTU)] were prepared by addition of red soil to distilled water, and then spiked with purified C. parvum oocysts. PET bottles containing the contaminated turbid waters were exposed to full sunlight for 4, 8 and 12 h. The samples were then concentrated by filtration and the oocyst viability was determined by inclusion ⁄ exclusion of the fluorogenic vital dye propidium iodide. results After an exposure time of 12 h (cumulative global dose of 28.28 MJ⁄m2; cumulative UV dose of 1037.06 kJ ⁄ m2) the oocyst viabilities were 11.54%, 25.96%, 41.50% and 52.80% for turbidity levels of 0, 5, 100 and 300 NTU, respectively, being significantly lower than the viability of theinitial isolate (P <0.01).conclusions SODIS method significantly reduced the potential viability of C. parvum oocysts on increasing the percentage of oocysts that took up the dye PI (indicator of cell wall integrity), although longer exposure periods appear to be required than those established for the bacterial pathogens usually tested in SODIS assays.
LanguageEnglish
Pages620-627
JournalTropical Medicine and International Health
Volume14
Issue number6
DOIs
Publication statusPublished - 10 Mar 2009

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disinfection
viability
turbidity
water
dye
red soil
iodide
method
pathogen
desert
assay
experiment

Keywords

  • SODIS
  • natural sunlight
  • PET bottles
  • turbid waters
  • Cryptosporidium
  • viability

Cite this

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title = "Efficacy of the solar water disinfection method in turbid waters experimentally contaminated withCryptosporidium parvumoocysts under real field conditions",
abstract = "bjective To investigate the efficacy of the solar water disinfection (SODIS) method for inactivating Cryptosporidium parvum oocysts in turbid waters using 1.5 l polyethylene terephthalate (PET) bottles under natural sunlight.methods All experiments were performed at the Plataforma Solar de Almerı ́a, located in the Tabernas Desert (Southern Spain) in July and October 2007. Turbid water samples [5, 100 and 300 nephelometric turbidity units (NTU)] were prepared by addition of red soil to distilled water, and then spiked with purified C. parvum oocysts. PET bottles containing the contaminated turbid waters were exposed to full sunlight for 4, 8 and 12 h. The samples were then concentrated by filtration and the oocyst viability was determined by inclusion ⁄ exclusion of the fluorogenic vital dye propidium iodide. results After an exposure time of 12 h (cumulative global dose of 28.28 MJ⁄m2; cumulative UV dose of 1037.06 kJ ⁄ m2) the oocyst viabilities were 11.54{\%}, 25.96{\%}, 41.50{\%} and 52.80{\%} for turbidity levels of 0, 5, 100 and 300 NTU, respectively, being significantly lower than the viability of theinitial isolate (P <0.01).conclusions SODIS method significantly reduced the potential viability of C. parvum oocysts on increasing the percentage of oocysts that took up the dye PI (indicator of cell wall integrity), although longer exposure periods appear to be required than those established for the bacterial pathogens usually tested in SODIS assays.",
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Efficacy of the solar water disinfection method in turbid waters experimentally contaminated withCryptosporidium parvumoocysts under real field conditions. / Gómez-Couso, H.; Fontán-Saínz, M.; Sichel, C.; Fernandez Ibanez, P; Ares-Mazás, E.

In: Tropical Medicine and International Health, Vol. 14, No. 6, 10.03.2009, p. 620-627.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Efficacy of the solar water disinfection method in turbid waters experimentally contaminated withCryptosporidium parvumoocysts under real field conditions

AU - Gómez-Couso, H.

AU - Fontán-Saínz, M.

AU - Sichel, C.

AU - Fernandez Ibanez, P

AU - Ares-Mazás, E.

PY - 2009/3/10

Y1 - 2009/3/10

N2 - bjective To investigate the efficacy of the solar water disinfection (SODIS) method for inactivating Cryptosporidium parvum oocysts in turbid waters using 1.5 l polyethylene terephthalate (PET) bottles under natural sunlight.methods All experiments were performed at the Plataforma Solar de Almerı ́a, located in the Tabernas Desert (Southern Spain) in July and October 2007. Turbid water samples [5, 100 and 300 nephelometric turbidity units (NTU)] were prepared by addition of red soil to distilled water, and then spiked with purified C. parvum oocysts. PET bottles containing the contaminated turbid waters were exposed to full sunlight for 4, 8 and 12 h. The samples were then concentrated by filtration and the oocyst viability was determined by inclusion ⁄ exclusion of the fluorogenic vital dye propidium iodide. results After an exposure time of 12 h (cumulative global dose of 28.28 MJ⁄m2; cumulative UV dose of 1037.06 kJ ⁄ m2) the oocyst viabilities were 11.54%, 25.96%, 41.50% and 52.80% for turbidity levels of 0, 5, 100 and 300 NTU, respectively, being significantly lower than the viability of theinitial isolate (P <0.01).conclusions SODIS method significantly reduced the potential viability of C. parvum oocysts on increasing the percentage of oocysts that took up the dye PI (indicator of cell wall integrity), although longer exposure periods appear to be required than those established for the bacterial pathogens usually tested in SODIS assays.

AB - bjective To investigate the efficacy of the solar water disinfection (SODIS) method for inactivating Cryptosporidium parvum oocysts in turbid waters using 1.5 l polyethylene terephthalate (PET) bottles under natural sunlight.methods All experiments were performed at the Plataforma Solar de Almerı ́a, located in the Tabernas Desert (Southern Spain) in July and October 2007. Turbid water samples [5, 100 and 300 nephelometric turbidity units (NTU)] were prepared by addition of red soil to distilled water, and then spiked with purified C. parvum oocysts. PET bottles containing the contaminated turbid waters were exposed to full sunlight for 4, 8 and 12 h. The samples were then concentrated by filtration and the oocyst viability was determined by inclusion ⁄ exclusion of the fluorogenic vital dye propidium iodide. results After an exposure time of 12 h (cumulative global dose of 28.28 MJ⁄m2; cumulative UV dose of 1037.06 kJ ⁄ m2) the oocyst viabilities were 11.54%, 25.96%, 41.50% and 52.80% for turbidity levels of 0, 5, 100 and 300 NTU, respectively, being significantly lower than the viability of theinitial isolate (P <0.01).conclusions SODIS method significantly reduced the potential viability of C. parvum oocysts on increasing the percentage of oocysts that took up the dye PI (indicator of cell wall integrity), although longer exposure periods appear to be required than those established for the bacterial pathogens usually tested in SODIS assays.

KW - SODIS

KW - natural sunlight

KW - PET bottles

KW - turbid waters

KW - Cryptosporidium

KW - viability

U2 - 10.1111/j.1365-3156.2009.02281.x

DO - 10.1111/j.1365-3156.2009.02281.x

M3 - Article

VL - 14

SP - 620

EP - 627

JO - Tropical Medicine and International Health

T2 - Tropical Medicine and International Health

JF - Tropical Medicine and International Health

SN - 1360-2276

IS - 6

ER -