Effects of tigerinin peptides on cytokine production by mouse peritoneal macrophages and spleen cells and by human peripheral blood mononuclear cells

Jelena M. Pantic, Milena Mechkarska, Miodrag L. Lukic, J. Michael Conlon

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

The tigerinins are a family of cationic, cyclic peptides of unknown biological function produced in the skins of diverse frog species. Tigerinin-1R (RVCSAIPLPICH.NH2) from Hoplobatrachus rugulosus (Dicroglossidae), tigerinin-1V (RICYAMWIPYPC) from Lithobates vaillanti (Ranidae), and tigerinin-1M (WCPPMIPLCSRF.NH2) from Xenopus muelleri (Pipidae) did not inhibit growth of Escherichia coli and Staphylococcus aureus at concentrations up to 500 μg/ml and were not hemolytic. Incubation of peritoneal macrophages from both BALB/c and C57BL/6 mice with tigerinin-1M, -1R and -1V (20 μg/ml) significantly (P < 0.05) increased production of the anti-inflammatory cytokine IL-10 and potentiated the stimulation produced by lipopolysaccharide (LPS). Incubation with the tigerinins (20 μg/ml) significantly increased production of IL-6 in LPS-stimulated macrophages from C57BL/6 mice but only tigerinin-1V potentiated IL-6 production in LPS-stimulated macrophages from BALB/c mice. The tigerinins did not have significant effects on the production of proinflammatory cytokines IL-12 and IL-23 by macrophages from BALB/c mice. In a population of mononuclear cells derived from mouse spleen, tigerinin-1M and -1V suppressed production of IFN-γ with no effect on IL-17 production and the three tigerinins enhanced IL-10 production. The three tigerinins (≤5 μg/ml) also significantly increased production of IL-10 in unstimulated and LPS-stimulated human peripheral blood mononuclear cells. The data indicate that the tigerinins may function as immunomodulatory host-defense peptides in frog skin.

Original languageEnglish
Pages (from-to)83-92
Number of pages10
JournalBiochimie
Volume101
Issue number1
DOIs
Publication statusPublished (in print/issue) - Jun 2014

Bibliographical note

Funding Information:
This work was supported by grants from United Arab Emirates University/National Research fund , the Terry Fox Fund for Cancer Research , and the Ministry of Education and Science, Belgrade, Serbia (grants ON 175071 , 175069 and 175103 ).

Keywords

  • Cytokine
  • Immunomodulatory peptide
  • Interleukin-10
  • Macrophage
  • Tigerinin

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