Effects of long-term exposure to nicotinamide and sodium butyrate on growth, viability, and the function of clonal insulin secreting cells

HK Liu, BD Green, Peter Flatt, Neville McClenaghan, Janie McCluskey

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The B vitamin nicotinamide (NIC), commonly known as niacin, is currently in trial as a potential means-of preventing Type 1 diabetes in first-degree relatives of affected individuals. Sodium butyrate (BUT) a common dietary micronutrient has also been reported to have beneficial effects on the differentiation and function of pancreatic beta cells. Cultured rat insulin-secreting BRIN-BD11 cells were used to investigate the effects of 3 days exposure to NIC (10 mM) and BUT (1 mM) both alone and in combination on beta cell function. Culture with NIC and/or BUT resulted in reduction of growth, insulin content and basal insulin secretion. BUT additionally decreased cell viability whilst NIC had no significant effect. Treatment with either agent abolished beta cell glucose sensitivity but insulin secretory responsiveness to a wide range of beta cell stimulators, including a depolarizing concentration of K+, elevation of Ca2+ and activation of adenylate cyclase and protein kinase C, were enhanced. These data illustrate that long term exposure to NIC and BUT has both positive and negative effects on the function of insulin-secreting cells.
LanguageEnglish
Pages61-68
JournalEndocrine Research
Volume30
Issue number1
DOIs
Publication statusPublished - 2004

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Butyric Acid
Niacinamide
Insulin-Secreting Cells
Butyrates
Growth
Insulin
Adenylate Kinase
Vitamin B Complex
Micronutrients
Niacin
Type 1 Diabetes Mellitus
Adenylyl Cyclases
Protein Kinase C
Insulin Resistance
Cell Survival
Glucose

Cite this

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abstract = "The B vitamin nicotinamide (NIC), commonly known as niacin, is currently in trial as a potential means-of preventing Type 1 diabetes in first-degree relatives of affected individuals. Sodium butyrate (BUT) a common dietary micronutrient has also been reported to have beneficial effects on the differentiation and function of pancreatic beta cells. Cultured rat insulin-secreting BRIN-BD11 cells were used to investigate the effects of 3 days exposure to NIC (10 mM) and BUT (1 mM) both alone and in combination on beta cell function. Culture with NIC and/or BUT resulted in reduction of growth, insulin content and basal insulin secretion. BUT additionally decreased cell viability whilst NIC had no significant effect. Treatment with either agent abolished beta cell glucose sensitivity but insulin secretory responsiveness to a wide range of beta cell stimulators, including a depolarizing concentration of K+, elevation of Ca2+ and activation of adenylate cyclase and protein kinase C, were enhanced. These data illustrate that long term exposure to NIC and BUT has both positive and negative effects on the function of insulin-secreting cells.",
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Effects of long-term exposure to nicotinamide and sodium butyrate on growth, viability, and the function of clonal insulin secreting cells. / Liu, HK; Green, BD; Flatt, Peter; McClenaghan, Neville; McCluskey, Janie.

In: Endocrine Research, Vol. 30, No. 1, 2004, p. 61-68.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Liu, HK

AU - Green, BD

AU - Flatt, Peter

AU - McClenaghan, Neville

AU - McCluskey, Janie

PY - 2004

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AB - The B vitamin nicotinamide (NIC), commonly known as niacin, is currently in trial as a potential means-of preventing Type 1 diabetes in first-degree relatives of affected individuals. Sodium butyrate (BUT) a common dietary micronutrient has also been reported to have beneficial effects on the differentiation and function of pancreatic beta cells. Cultured rat insulin-secreting BRIN-BD11 cells were used to investigate the effects of 3 days exposure to NIC (10 mM) and BUT (1 mM) both alone and in combination on beta cell function. Culture with NIC and/or BUT resulted in reduction of growth, insulin content and basal insulin secretion. BUT additionally decreased cell viability whilst NIC had no significant effect. Treatment with either agent abolished beta cell glucose sensitivity but insulin secretory responsiveness to a wide range of beta cell stimulators, including a depolarizing concentration of K+, elevation of Ca2+ and activation of adenylate cyclase and protein kinase C, were enhanced. These data illustrate that long term exposure to NIC and BUT has both positive and negative effects on the function of insulin-secreting cells.

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