and the ethanolic extract (EE) from Artemisia judaica L., a Jordanian
medicinal plant, exhibit a potent anti‑angiogenic and anti‑inflammatory
activities. Angiogenesis and inflammation processes are known to
play a role in benign prostatic hyperplasia (BPH), a disease associated
with aging in men. Objectives: The present study aimed to address
the effects of EO and EE on experimentally‑induced BPH in rats.
Materials and Methods: Four experimental groups were assigned with
six rats in each group, including the corn oil vehicle as a control. The three
other groups were induced to develop BPH by testosterone injection.
The BPH rats were randomized into the BPH‑untreated group, BPH‑EO
treated group (200 mg/kg/subcutaneously) and BPH‑EE treated group
(500 mg/kg/orally). Results: The prostate weight/body ratio and epithelial
thickness showed a significant reduction in the EO and EE treated
groups compared to the BPH untreated. In addition, mRNA expression
levels of the proliferating marker (proliferating cell nuclear antigen),
the angiogenesis marker (vascular endothelial growth factor‑A) and
interleukin‑6; an inflammatory cytokine, were significantly down‑regulated
in the BPH groups that were treated with EE or EO. Conclusion: Our
results indicated that in experimentally‑induced BPH, EO and EE from
A. judaica ameliorate BPH development by inhibiting prostatic cell
proliferation, angiogenesis, and inflammation.