Effects of α-Lipoic Acid on Peripheral Mononuclear Cell and Mitochondrial DNA Damage after Isolated Muscle Contractions

MC Fogarty, G Devito, CM Hughes, G Burke, JC Brown, J McEneny, D Brown, Conor McClean, Gareth Davison

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Introduction: Although pharmacological antioxidants have previously been investigated for a prophylactic effect against exercise oxidative stress, is not known if α-lipoic acid supplementation can protect against DNA damage following high intensity isolated quadriceps exercise. This randomised controlled investigation was designed to test the hypothesis that fourteen days of α-lipoic acid supplementation can attenuate exercise-induced oxidative stress. Methods: Twelve (n=12) apparently healthy male participants (age 28 ± 10 years, stature 177 ± 12 cm and body mass 81 ± 15 kg) were randomly assigned to receive either a daily supplement of 1000mg of α-lipoic acid (2 × 500mg tablets) for fourteen days (n = 6) or receive no supplement (n = 6) in a double blinded experimental approach. Blood and muscle biopsy tissue samples were taken at rest and following the completion of 100 isolated and continuous maximal knee extension (minimum force = 200 N, speed of contraction = 60°/sec). Results: Exercise increased mitochondrial 8-OHdG concentration in both groups (P <0.05 vs. rest) with a concomitant decrease in total antioxidant capacity (P <0.05 vs. rest). There was a marked increase in blood total antioxidant capacity following oral α-lipoic acid supplementation (P <0.05 vs. non-supplemented), while DNA damage (Comet assay and 8-OHdG) lipid peroxidation and hydrogen peroxide increased following exercise in the non-supplemented group only (P <0.05 vs. supplemented). Exercise increased protein oxidation in both groups (P <0.05 vs. rest). Conclusion: These findings suggest that short-term α-lipoic acid supplementation can selectively protect DNA (but not in muscle mitochondria) and lipids against exercise-induced oxidative stress.
LanguageEnglish
Pages1469-1477
Number of pages1
JournalMedicine & Science in Sports & Exercise
Volume45
Issue number8
DOIs
Publication statusPublished - Aug 2013

Fingerprint

Thioctic Acid
Muscle Contraction
Mitochondrial DNA
DNA Damage
Exercise
Oxidative Stress
Antioxidants
Muscle Mitochondrion
Comet Assay
Lipid Peroxides
Hydrogen Peroxide
Lipid Peroxidation
Tablets
Knee
Healthy Volunteers
Pharmacology
Lipids
Biopsy
Muscles
DNA

Cite this

Fogarty, MC ; Devito, G ; Hughes, CM ; Burke, G ; Brown, JC ; McEneny, J ; Brown, D ; McClean, Conor ; Davison, Gareth. / Effects of α-Lipoic Acid on Peripheral Mononuclear Cell and Mitochondrial DNA Damage after Isolated Muscle Contractions. In: Medicine & Science in Sports & Exercise. 2013 ; Vol. 45, No. 8. pp. 1469-1477.
@article{bfba06a98770445ba12d42aabb3e66bf,
title = "Effects of α-Lipoic Acid on Peripheral Mononuclear Cell and Mitochondrial DNA Damage after Isolated Muscle Contractions",
abstract = "Introduction: Although pharmacological antioxidants have previously been investigated for a prophylactic effect against exercise oxidative stress, is not known if α-lipoic acid supplementation can protect against DNA damage following high intensity isolated quadriceps exercise. This randomised controlled investigation was designed to test the hypothesis that fourteen days of α-lipoic acid supplementation can attenuate exercise-induced oxidative stress. Methods: Twelve (n=12) apparently healthy male participants (age 28 ± 10 years, stature 177 ± 12 cm and body mass 81 ± 15 kg) were randomly assigned to receive either a daily supplement of 1000mg of α-lipoic acid (2 × 500mg tablets) for fourteen days (n = 6) or receive no supplement (n = 6) in a double blinded experimental approach. Blood and muscle biopsy tissue samples were taken at rest and following the completion of 100 isolated and continuous maximal knee extension (minimum force = 200 N, speed of contraction = 60°/sec). Results: Exercise increased mitochondrial 8-OHdG concentration in both groups (P <0.05 vs. rest) with a concomitant decrease in total antioxidant capacity (P <0.05 vs. rest). There was a marked increase in blood total antioxidant capacity following oral α-lipoic acid supplementation (P <0.05 vs. non-supplemented), while DNA damage (Comet assay and 8-OHdG) lipid peroxidation and hydrogen peroxide increased following exercise in the non-supplemented group only (P <0.05 vs. supplemented). Exercise increased protein oxidation in both groups (P <0.05 vs. rest). Conclusion: These findings suggest that short-term α-lipoic acid supplementation can selectively protect DNA (but not in muscle mitochondria) and lipids against exercise-induced oxidative stress.",
author = "MC Fogarty and G Devito and CM Hughes and G Burke and JC Brown and J McEneny and D Brown and Conor McClean and Gareth Davison",
year = "2013",
month = "8",
doi = "10.1249/MSS.0b013e31828bf31e",
language = "English",
volume = "45",
pages = "1469--1477",
journal = "Medicine and Science in Sports and Exercise",
issn = "0195-9131",
number = "8",

}

Effects of α-Lipoic Acid on Peripheral Mononuclear Cell and Mitochondrial DNA Damage after Isolated Muscle Contractions. / Fogarty, MC; Devito, G; Hughes, CM; Burke, G; Brown, JC; McEneny, J; Brown, D; McClean, Conor; Davison, Gareth.

In: Medicine & Science in Sports & Exercise, Vol. 45, No. 8, 08.2013, p. 1469-1477.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of α-Lipoic Acid on Peripheral Mononuclear Cell and Mitochondrial DNA Damage after Isolated Muscle Contractions

AU - Fogarty, MC

AU - Devito, G

AU - Hughes, CM

AU - Burke, G

AU - Brown, JC

AU - McEneny, J

AU - Brown, D

AU - McClean, Conor

AU - Davison, Gareth

PY - 2013/8

Y1 - 2013/8

N2 - Introduction: Although pharmacological antioxidants have previously been investigated for a prophylactic effect against exercise oxidative stress, is not known if α-lipoic acid supplementation can protect against DNA damage following high intensity isolated quadriceps exercise. This randomised controlled investigation was designed to test the hypothesis that fourteen days of α-lipoic acid supplementation can attenuate exercise-induced oxidative stress. Methods: Twelve (n=12) apparently healthy male participants (age 28 ± 10 years, stature 177 ± 12 cm and body mass 81 ± 15 kg) were randomly assigned to receive either a daily supplement of 1000mg of α-lipoic acid (2 × 500mg tablets) for fourteen days (n = 6) or receive no supplement (n = 6) in a double blinded experimental approach. Blood and muscle biopsy tissue samples were taken at rest and following the completion of 100 isolated and continuous maximal knee extension (minimum force = 200 N, speed of contraction = 60°/sec). Results: Exercise increased mitochondrial 8-OHdG concentration in both groups (P <0.05 vs. rest) with a concomitant decrease in total antioxidant capacity (P <0.05 vs. rest). There was a marked increase in blood total antioxidant capacity following oral α-lipoic acid supplementation (P <0.05 vs. non-supplemented), while DNA damage (Comet assay and 8-OHdG) lipid peroxidation and hydrogen peroxide increased following exercise in the non-supplemented group only (P <0.05 vs. supplemented). Exercise increased protein oxidation in both groups (P <0.05 vs. rest). Conclusion: These findings suggest that short-term α-lipoic acid supplementation can selectively protect DNA (but not in muscle mitochondria) and lipids against exercise-induced oxidative stress.

AB - Introduction: Although pharmacological antioxidants have previously been investigated for a prophylactic effect against exercise oxidative stress, is not known if α-lipoic acid supplementation can protect against DNA damage following high intensity isolated quadriceps exercise. This randomised controlled investigation was designed to test the hypothesis that fourteen days of α-lipoic acid supplementation can attenuate exercise-induced oxidative stress. Methods: Twelve (n=12) apparently healthy male participants (age 28 ± 10 years, stature 177 ± 12 cm and body mass 81 ± 15 kg) were randomly assigned to receive either a daily supplement of 1000mg of α-lipoic acid (2 × 500mg tablets) for fourteen days (n = 6) or receive no supplement (n = 6) in a double blinded experimental approach. Blood and muscle biopsy tissue samples were taken at rest and following the completion of 100 isolated and continuous maximal knee extension (minimum force = 200 N, speed of contraction = 60°/sec). Results: Exercise increased mitochondrial 8-OHdG concentration in both groups (P <0.05 vs. rest) with a concomitant decrease in total antioxidant capacity (P <0.05 vs. rest). There was a marked increase in blood total antioxidant capacity following oral α-lipoic acid supplementation (P <0.05 vs. non-supplemented), while DNA damage (Comet assay and 8-OHdG) lipid peroxidation and hydrogen peroxide increased following exercise in the non-supplemented group only (P <0.05 vs. supplemented). Exercise increased protein oxidation in both groups (P <0.05 vs. rest). Conclusion: These findings suggest that short-term α-lipoic acid supplementation can selectively protect DNA (but not in muscle mitochondria) and lipids against exercise-induced oxidative stress.

U2 - 10.1249/MSS.0b013e31828bf31e

DO - 10.1249/MSS.0b013e31828bf31e

M3 - Article

VL - 45

SP - 1469

EP - 1477

JO - Medicine and Science in Sports and Exercise

T2 - Medicine and Science in Sports and Exercise

JF - Medicine and Science in Sports and Exercise

SN - 0195-9131

IS - 8

ER -