Effect of vitamin C supplementation on hepatic cytochrome P450 mixed-function oxidase activity in streptozotocin-diabetic rats

J Clarke; J Snelling; C Ioannides; Peter Flatt; CR Barnett

Effect of vitamin C supplementation on hepatic cytochrome P450 mixed-function oxidase activity in streptozotocin-diabetic rats

J Clarke
, J Snelling
, C Ioannides
, Peter Flatt
, CR Barnett

Research output: Contribution to journal › Article › peer-review

Abstract

The effect of vitamin C supplementation on hepatic cytochrome P450 expression was investigated in streptozotocin (STZ) diabetic male Wistar Albino rats. STZ-treated rats displayed the usual characteristics of diabetes including; hyperphagia, polydipsia, decreased body weight gain and also the increased expression and activity of hepatic CYP1A, 2B, 2E and 4A proteins. Vitamin C administration in drinking water (2% w/v) was associated with significant decreases in the levels of hyperglycaemia (P <0.05), glycosylated haemoglobin (P <0.05), hyperlipidaemia (P <0.001), and hyperketonaemia (P <0.001) associated with STZ-diabetes. Vitamin C-treatment selectively reduced the activity and expression of CYP2E proteins (P <0.001). These effects on CYP2E expression may be mediated by the reduced levels of circulating ketone bodies, however, a direct effect on CYP2E expression in diabetes cannot be discounted.

Original language	English
Pages (from-to)	249-256
Journal	Toxicology Letters
Volume	89
Issue number	3
Publication status	Published (in print/issue) - Dec 1996

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title = "Effect of vitamin C supplementation on hepatic cytochrome P450 mixed-function oxidase activity in streptozotocin-diabetic rats",

abstract = "The effect of vitamin C supplementation on hepatic cytochrome P450 expression was investigated in streptozotocin (STZ) diabetic male Wistar Albino rats. STZ-treated rats displayed the usual characteristics of diabetes including; hyperphagia, polydipsia, decreased body weight gain and also the increased expression and activity of hepatic CYP1A, 2B, 2E and 4A proteins. Vitamin C administration in drinking water (2\% w/v) was associated with significant decreases in the levels of hyperglycaemia (P <0.05), glycosylated haemoglobin (P <0.05), hyperlipidaemia (P <0.001), and hyperketonaemia (P <0.001) associated with STZ-diabetes. Vitamin C-treatment selectively reduced the activity and expression of CYP2E proteins (P <0.001). These effects on CYP2E expression may be mediated by the reduced levels of circulating ketone bodies, however, a direct effect on CYP2E expression in diabetes cannot be discounted.",

author = "J Clarke and J Snelling and C Ioannides and Peter Flatt and CR Barnett",

year = "1996",

month = dec,

language = "English",

volume = "89",

pages = "249--256",

journal = "Toxicology Letters",

issn = "1879-3169",

publisher = "Elsevier Ireland Ltd",

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TY - JOUR

T1 - Effect of vitamin C supplementation on hepatic cytochrome P450 mixed-function oxidase activity in streptozotocin-diabetic rats

AU - Clarke, J

AU - Snelling, J

AU - Ioannides, C

AU - Flatt, Peter

AU - Barnett, CR

PY - 1996/12

Y1 - 1996/12

N2 - The effect of vitamin C supplementation on hepatic cytochrome P450 expression was investigated in streptozotocin (STZ) diabetic male Wistar Albino rats. STZ-treated rats displayed the usual characteristics of diabetes including; hyperphagia, polydipsia, decreased body weight gain and also the increased expression and activity of hepatic CYP1A, 2B, 2E and 4A proteins. Vitamin C administration in drinking water (2% w/v) was associated with significant decreases in the levels of hyperglycaemia (P <0.05), glycosylated haemoglobin (P <0.05), hyperlipidaemia (P <0.001), and hyperketonaemia (P <0.001) associated with STZ-diabetes. Vitamin C-treatment selectively reduced the activity and expression of CYP2E proteins (P <0.001). These effects on CYP2E expression may be mediated by the reduced levels of circulating ketone bodies, however, a direct effect on CYP2E expression in diabetes cannot be discounted.

AB - The effect of vitamin C supplementation on hepatic cytochrome P450 expression was investigated in streptozotocin (STZ) diabetic male Wistar Albino rats. STZ-treated rats displayed the usual characteristics of diabetes including; hyperphagia, polydipsia, decreased body weight gain and also the increased expression and activity of hepatic CYP1A, 2B, 2E and 4A proteins. Vitamin C administration in drinking water (2% w/v) was associated with significant decreases in the levels of hyperglycaemia (P <0.05), glycosylated haemoglobin (P <0.05), hyperlipidaemia (P <0.001), and hyperketonaemia (P <0.001) associated with STZ-diabetes. Vitamin C-treatment selectively reduced the activity and expression of CYP2E proteins (P <0.001). These effects on CYP2E expression may be mediated by the reduced levels of circulating ketone bodies, however, a direct effect on CYP2E expression in diabetes cannot be discounted.

M3 - Article

SN - 1879-3169

VL - 89

SP - 249

EP - 256

JO - Toxicology Letters

JF - Toxicology Letters

IS - 3

ER -

Effect of vitamin C supplementation on hepatic cytochrome P450 mixed-function oxidase activity in streptozotocin-diabetic rats

Abstract

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