Effect of vitamin C supplementation on hepatic cytochrome P450 mixed-function oxidase activity in streptozotocin-diabetic rats

J Clarke, J Snelling, C Ioannides, Peter Flatt, CR Barnett

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Abstract

The effect of vitamin C supplementation on hepatic cytochrome P450 expression was investigated in streptozotocin (STZ) diabetic male Wistar Albino rats. STZ-treated rats displayed the usual characteristics of diabetes including; hyperphagia, polydipsia, decreased body weight gain and also the increased expression and activity of hepatic CYP1A, 2B, 2E and 4A proteins. Vitamin C administration in drinking water (2% w/v) was associated with significant decreases in the levels of hyperglycaemia (P <0.05), glycosylated haemoglobin (P <0.05), hyperlipidaemia (P <0.001), and hyperketonaemia (P <0.001) associated with STZ-diabetes. Vitamin C-treatment selectively reduced the activity and expression of CYP2E proteins (P <0.001). These effects on CYP2E expression may be mediated by the reduced levels of circulating ketone bodies, however, a direct effect on CYP2E expression in diabetes cannot be discounted.
LanguageEnglish
Pages249-256
JournalToxicology Letters
Volume89
Issue number3
Publication statusPublished - Dec 1996

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Streptozocin
Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Ascorbic Acid
Liver
Polydipsia
Ketone Bodies
Hyperphagia
Experimental Diabetes Mellitus
Glycosylated Hemoglobin A
Hyperlipidemias
Drinking Water
Hyperglycemia
Weight Gain
Wistar Rats
Proteins
Body Weight

Cite this

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title = "Effect of vitamin C supplementation on hepatic cytochrome P450 mixed-function oxidase activity in streptozotocin-diabetic rats",
abstract = "The effect of vitamin C supplementation on hepatic cytochrome P450 expression was investigated in streptozotocin (STZ) diabetic male Wistar Albino rats. STZ-treated rats displayed the usual characteristics of diabetes including; hyperphagia, polydipsia, decreased body weight gain and also the increased expression and activity of hepatic CYP1A, 2B, 2E and 4A proteins. Vitamin C administration in drinking water (2{\%} w/v) was associated with significant decreases in the levels of hyperglycaemia (P <0.05), glycosylated haemoglobin (P <0.05), hyperlipidaemia (P <0.001), and hyperketonaemia (P <0.001) associated with STZ-diabetes. Vitamin C-treatment selectively reduced the activity and expression of CYP2E proteins (P <0.001). These effects on CYP2E expression may be mediated by the reduced levels of circulating ketone bodies, however, a direct effect on CYP2E expression in diabetes cannot be discounted.",
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Effect of vitamin C supplementation on hepatic cytochrome P450 mixed-function oxidase activity in streptozotocin-diabetic rats. / Clarke, J; Snelling, J; Ioannides, C; Flatt, Peter; Barnett, CR.

In: Toxicology Letters, Vol. 89, No. 3, 12.1996, p. 249-256.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of vitamin C supplementation on hepatic cytochrome P450 mixed-function oxidase activity in streptozotocin-diabetic rats

AU - Clarke, J

AU - Snelling, J

AU - Ioannides, C

AU - Flatt, Peter

AU - Barnett, CR

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AB - The effect of vitamin C supplementation on hepatic cytochrome P450 expression was investigated in streptozotocin (STZ) diabetic male Wistar Albino rats. STZ-treated rats displayed the usual characteristics of diabetes including; hyperphagia, polydipsia, decreased body weight gain and also the increased expression and activity of hepatic CYP1A, 2B, 2E and 4A proteins. Vitamin C administration in drinking water (2% w/v) was associated with significant decreases in the levels of hyperglycaemia (P <0.05), glycosylated haemoglobin (P <0.05), hyperlipidaemia (P <0.001), and hyperketonaemia (P <0.001) associated with STZ-diabetes. Vitamin C-treatment selectively reduced the activity and expression of CYP2E proteins (P <0.001). These effects on CYP2E expression may be mediated by the reduced levels of circulating ketone bodies, however, a direct effect on CYP2E expression in diabetes cannot be discounted.

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