Effect of vitamin C supplementation on hepatic cytochrome P450 mixed-function oxidase activity in streptozotocin-diabetic rats

J Clarke, J Snelling, C Ioannides, Peter Flatt, CR Barnett

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Abstract

The effect of vitamin C supplementation on hepatic cytochrome P450 expression was investigated in streptozotocin (STZ) diabetic male Wistar Albino rats. STZ-treated rats displayed the usual characteristics of diabetes including; hyperphagia, polydipsia, decreased body weight gain and also the increased expression and activity of hepatic CYP1A, 2B, 2E and 4A proteins. Vitamin C administration in drinking water (2% w/v) was associated with significant decreases in the levels of hyperglycaemia (P <0.05), glycosylated haemoglobin (P <0.05), hyperlipidaemia (P <0.001), and hyperketonaemia (P <0.001) associated with STZ-diabetes. Vitamin C-treatment selectively reduced the activity and expression of CYP2E proteins (P <0.001). These effects on CYP2E expression may be mediated by the reduced levels of circulating ketone bodies, however, a direct effect on CYP2E expression in diabetes cannot be discounted.
Original languageEnglish
Pages (from-to)249-256
JournalToxicology Letters
Volume89
Issue number3
Publication statusPublished - Dec 1996

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