Effect of simulated gastrointestinal digestion and fermentation on polyphenolic content and bioactivity of brown seaweed phlorotannin-rich extracts.

Giulia Corona, Maria Magdalena Coman, Yuxuan Guo, Sarah Hotchkiss, Chris Gill, Parveen Yaqoob, Jeremy P.E. Spencer, Ian Rowland

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Scope: Unlike other classes of polyphenols, there is a lack of knowledge regarding brown seaweed phlorotannins and their bioactivity. We investigated the impact of in vitro gastrointestinal digestion and colonic fermentation on the bioactivity of a seaweed phlorotannin extract from Ascophyllum nodosum and its high molecular weight (HMW) and low molecular weight (LMW) fractions.Methods and Results: The highest phlorotannin and total polyphenol (TP) concentration was observed in the HMW fraction. Antioxidant capacity broadly followed phlorotannin and TP levels, with HMW having the highest activity. Both gastrointestinal digestion (GID) and colonic fermentation (CF) significantly affected phlorotannin and TP levels, and antioxidant capacity of the extract and fractions. Despite this, in HT-29 cells, all GID extracts significantly inhibit cell growth, whereas CF extracts effectively counteracted H2O2 induced DNA damage.Conclusion: Although phlorotannins, TP levels and antioxidant power of the extracts were strongly reduced after in vitro digestion and fermentation, their anti-genotoxic activity and cell growth inhibitory effect in colon HT-29 cells was maintained and enhanced. HMW was the most effective fraction, indicating that the high molecular weight phlorotannins potentially exert a stronger beneficial effect in the colon.
LanguageEnglish
JournalMolecular Nutrition and Food Research
Volume61
Issue number11
Early online date29 Aug 2017
DOIs
Publication statusPublished - Nov 2017

Fingerprint

Seaweed
Phaeophyceae
Fermentation
Polyphenols
Digestion
polyphenols
Molecular Weight
digestion
fermentation
molecular weight
extracts
HT29 Cells
Antioxidants
antioxidants
colon
cell growth
Ascophyllum
Colon
Ascophyllum nodosum
in vitro digestion

Keywords

  • Digestion
  • Fermentation
  • Phlorotannins
  • Polyphenols
  • Seaweeds

Cite this

Corona, Giulia ; Coman, Maria Magdalena ; Guo, Yuxuan ; Hotchkiss, Sarah ; Gill, Chris ; Yaqoob, Parveen ; Spencer, Jeremy P.E. ; Rowland, Ian. / Effect of simulated gastrointestinal digestion and fermentation on polyphenolic content and bioactivity of brown seaweed phlorotannin-rich extracts. In: Molecular Nutrition and Food Research. 2017 ; Vol. 61, No. 11.
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abstract = "Scope: Unlike other classes of polyphenols, there is a lack of knowledge regarding brown seaweed phlorotannins and their bioactivity. We investigated the impact of in vitro gastrointestinal digestion and colonic fermentation on the bioactivity of a seaweed phlorotannin extract from Ascophyllum nodosum and its high molecular weight (HMW) and low molecular weight (LMW) fractions.Methods and Results: The highest phlorotannin and total polyphenol (TP) concentration was observed in the HMW fraction. Antioxidant capacity broadly followed phlorotannin and TP levels, with HMW having the highest activity. Both gastrointestinal digestion (GID) and colonic fermentation (CF) significantly affected phlorotannin and TP levels, and antioxidant capacity of the extract and fractions. Despite this, in HT-29 cells, all GID extracts significantly inhibit cell growth, whereas CF extracts effectively counteracted H2O2 induced DNA damage.Conclusion: Although phlorotannins, TP levels and antioxidant power of the extracts were strongly reduced after in vitro digestion and fermentation, their anti-genotoxic activity and cell growth inhibitory effect in colon HT-29 cells was maintained and enhanced. HMW was the most effective fraction, indicating that the high molecular weight phlorotannins potentially exert a stronger beneficial effect in the colon.",
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Effect of simulated gastrointestinal digestion and fermentation on polyphenolic content and bioactivity of brown seaweed phlorotannin-rich extracts. / Corona, Giulia; Coman, Maria Magdalena; Guo, Yuxuan; Hotchkiss, Sarah; Gill, Chris; Yaqoob, Parveen; Spencer, Jeremy P.E.; Rowland, Ian.

In: Molecular Nutrition and Food Research, Vol. 61, No. 11, 11.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of simulated gastrointestinal digestion and fermentation on polyphenolic content and bioactivity of brown seaweed phlorotannin-rich extracts.

AU - Corona, Giulia

AU - Coman, Maria Magdalena

AU - Guo, Yuxuan

AU - Hotchkiss, Sarah

AU - Gill, Chris

AU - Yaqoob, Parveen

AU - Spencer, Jeremy P.E.

AU - Rowland, Ian

PY - 2017/11

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N2 - Scope: Unlike other classes of polyphenols, there is a lack of knowledge regarding brown seaweed phlorotannins and their bioactivity. We investigated the impact of in vitro gastrointestinal digestion and colonic fermentation on the bioactivity of a seaweed phlorotannin extract from Ascophyllum nodosum and its high molecular weight (HMW) and low molecular weight (LMW) fractions.Methods and Results: The highest phlorotannin and total polyphenol (TP) concentration was observed in the HMW fraction. Antioxidant capacity broadly followed phlorotannin and TP levels, with HMW having the highest activity. Both gastrointestinal digestion (GID) and colonic fermentation (CF) significantly affected phlorotannin and TP levels, and antioxidant capacity of the extract and fractions. Despite this, in HT-29 cells, all GID extracts significantly inhibit cell growth, whereas CF extracts effectively counteracted H2O2 induced DNA damage.Conclusion: Although phlorotannins, TP levels and antioxidant power of the extracts were strongly reduced after in vitro digestion and fermentation, their anti-genotoxic activity and cell growth inhibitory effect in colon HT-29 cells was maintained and enhanced. HMW was the most effective fraction, indicating that the high molecular weight phlorotannins potentially exert a stronger beneficial effect in the colon.

AB - Scope: Unlike other classes of polyphenols, there is a lack of knowledge regarding brown seaweed phlorotannins and their bioactivity. We investigated the impact of in vitro gastrointestinal digestion and colonic fermentation on the bioactivity of a seaweed phlorotannin extract from Ascophyllum nodosum and its high molecular weight (HMW) and low molecular weight (LMW) fractions.Methods and Results: The highest phlorotannin and total polyphenol (TP) concentration was observed in the HMW fraction. Antioxidant capacity broadly followed phlorotannin and TP levels, with HMW having the highest activity. Both gastrointestinal digestion (GID) and colonic fermentation (CF) significantly affected phlorotannin and TP levels, and antioxidant capacity of the extract and fractions. Despite this, in HT-29 cells, all GID extracts significantly inhibit cell growth, whereas CF extracts effectively counteracted H2O2 induced DNA damage.Conclusion: Although phlorotannins, TP levels and antioxidant power of the extracts were strongly reduced after in vitro digestion and fermentation, their anti-genotoxic activity and cell growth inhibitory effect in colon HT-29 cells was maintained and enhanced. HMW was the most effective fraction, indicating that the high molecular weight phlorotannins potentially exert a stronger beneficial effect in the colon.

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KW - Phlorotannins

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KW - Seaweeds

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