Effect of fecal water on an in vitro model of colonic mucosal barrier function

Chris I. R. Gill, Patricia Heavey, Eileen McConville, Ian Bradbury, Caroline Faessler, Susanne Mueller, Alberto Cresci, Joel Dore, Elisabeth Norin, Ian Rowland

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    Fecal water (FW) has been shown to exert, in cultured cells, cytotoxic and genotoxic effects that have implications for colorectal cancer (CRC) risk. We have investigated a further biological activity of FW, namely, the ability to affect gap junctions in CACO2 cell monolayers as an index of mucosal barrier function, which is known to be disrupted in cancer. FW samples fi-om healthy, free-living, European subjects that were divided into two broad age groups, adult (40 +/- 9.7 yr; n = 53) and elderly (76 +/- 7.5 yr; n = 55) were tested for effects on gap junction using the transepithelial resistance (TER) assay. Overall, treatment of CACO2 cells with FW samples fi-om adults increased TER (+ 4 %), whereas FW from elderly subjects decreased TER (-5%); the difference between the two groups was significant (P < 0.05). We also measured several components of FW potentially associated with modulation of TER, namely, short-chain fatty acid (SCFA) and ammonia. SCFAs (propionic, acetic, and n-butyric) were significantly lower in the elderly population (-30%, -35%, and -21%, respectively, all P pound 0.01). We consider that FW modulation of in vitro epithelial barrier function is a potentially useful noninvasive biomarker, but it requires further validation to establish its relationship to CRC risk.
    LanguageEnglish
    Pages59-65
    JournalNUTRITION AND CANCER-AN INTERNATIONAL JOURNAL
    Volume57
    Issue number1
    Publication statusPublished - 2007

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    Water
    Gap Junctions
    Colorectal Neoplasms
    Volatile Fatty Acids
    In Vitro Techniques
    Ammonia
    Acetic Acid
    Cultured Cells
    Age Groups
    Biomarkers
    Population
    Neoplasms
    Therapeutics

    Cite this

    Gill, C. I. R., Heavey, P., McConville, E., Bradbury, I., Faessler, C., Mueller, S., ... Rowland, I. (2007). Effect of fecal water on an in vitro model of colonic mucosal barrier function. NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 57(1), 59-65.
    Gill, Chris I. R. ; Heavey, Patricia ; McConville, Eileen ; Bradbury, Ian ; Faessler, Caroline ; Mueller, Susanne ; Cresci, Alberto ; Dore, Joel ; Norin, Elisabeth ; Rowland, Ian. / Effect of fecal water on an in vitro model of colonic mucosal barrier function. In: NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL. 2007 ; Vol. 57, No. 1. pp. 59-65.
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    title = "Effect of fecal water on an in vitro model of colonic mucosal barrier function",
    abstract = "Fecal water (FW) has been shown to exert, in cultured cells, cytotoxic and genotoxic effects that have implications for colorectal cancer (CRC) risk. We have investigated a further biological activity of FW, namely, the ability to affect gap junctions in CACO2 cell monolayers as an index of mucosal barrier function, which is known to be disrupted in cancer. FW samples fi-om healthy, free-living, European subjects that were divided into two broad age groups, adult (40 +/- 9.7 yr; n = 53) and elderly (76 +/- 7.5 yr; n = 55) were tested for effects on gap junction using the transepithelial resistance (TER) assay. Overall, treatment of CACO2 cells with FW samples fi-om adults increased TER (+ 4 {\%}), whereas FW from elderly subjects decreased TER (-5{\%}); the difference between the two groups was significant (P < 0.05). We also measured several components of FW potentially associated with modulation of TER, namely, short-chain fatty acid (SCFA) and ammonia. SCFAs (propionic, acetic, and n-butyric) were significantly lower in the elderly population (-30{\%}, -35{\%}, and -21{\%}, respectively, all P pound 0.01). We consider that FW modulation of in vitro epithelial barrier function is a potentially useful noninvasive biomarker, but it requires further validation to establish its relationship to CRC risk.",
    author = "Gill, {Chris I. R.} and Patricia Heavey and Eileen McConville and Ian Bradbury and Caroline Faessler and Susanne Mueller and Alberto Cresci and Joel Dore and Elisabeth Norin and Ian Rowland",
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    Gill, CIR, Heavey, P, McConville, E, Bradbury, I, Faessler, C, Mueller, S, Cresci, A, Dore, J, Norin, E & Rowland, I 2007, 'Effect of fecal water on an in vitro model of colonic mucosal barrier function', NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, vol. 57, no. 1, pp. 59-65.

    Effect of fecal water on an in vitro model of colonic mucosal barrier function. / Gill, Chris I. R.; Heavey, Patricia; McConville, Eileen; Bradbury, Ian; Faessler, Caroline; Mueller, Susanne; Cresci, Alberto; Dore, Joel; Norin, Elisabeth; Rowland, Ian.

    In: NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, Vol. 57, No. 1, 2007, p. 59-65.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Effect of fecal water on an in vitro model of colonic mucosal barrier function

    AU - Gill, Chris I. R.

    AU - Heavey, Patricia

    AU - McConville, Eileen

    AU - Bradbury, Ian

    AU - Faessler, Caroline

    AU - Mueller, Susanne

    AU - Cresci, Alberto

    AU - Dore, Joel

    AU - Norin, Elisabeth

    AU - Rowland, Ian

    PY - 2007

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    N2 - Fecal water (FW) has been shown to exert, in cultured cells, cytotoxic and genotoxic effects that have implications for colorectal cancer (CRC) risk. We have investigated a further biological activity of FW, namely, the ability to affect gap junctions in CACO2 cell monolayers as an index of mucosal barrier function, which is known to be disrupted in cancer. FW samples fi-om healthy, free-living, European subjects that were divided into two broad age groups, adult (40 +/- 9.7 yr; n = 53) and elderly (76 +/- 7.5 yr; n = 55) were tested for effects on gap junction using the transepithelial resistance (TER) assay. Overall, treatment of CACO2 cells with FW samples fi-om adults increased TER (+ 4 %), whereas FW from elderly subjects decreased TER (-5%); the difference between the two groups was significant (P < 0.05). We also measured several components of FW potentially associated with modulation of TER, namely, short-chain fatty acid (SCFA) and ammonia. SCFAs (propionic, acetic, and n-butyric) were significantly lower in the elderly population (-30%, -35%, and -21%, respectively, all P pound 0.01). We consider that FW modulation of in vitro epithelial barrier function is a potentially useful noninvasive biomarker, but it requires further validation to establish its relationship to CRC risk.

    AB - Fecal water (FW) has been shown to exert, in cultured cells, cytotoxic and genotoxic effects that have implications for colorectal cancer (CRC) risk. We have investigated a further biological activity of FW, namely, the ability to affect gap junctions in CACO2 cell monolayers as an index of mucosal barrier function, which is known to be disrupted in cancer. FW samples fi-om healthy, free-living, European subjects that were divided into two broad age groups, adult (40 +/- 9.7 yr; n = 53) and elderly (76 +/- 7.5 yr; n = 55) were tested for effects on gap junction using the transepithelial resistance (TER) assay. Overall, treatment of CACO2 cells with FW samples fi-om adults increased TER (+ 4 %), whereas FW from elderly subjects decreased TER (-5%); the difference between the two groups was significant (P < 0.05). We also measured several components of FW potentially associated with modulation of TER, namely, short-chain fatty acid (SCFA) and ammonia. SCFAs (propionic, acetic, and n-butyric) were significantly lower in the elderly population (-30%, -35%, and -21%, respectively, all P pound 0.01). We consider that FW modulation of in vitro epithelial barrier function is a potentially useful noninvasive biomarker, but it requires further validation to establish its relationship to CRC risk.

    M3 - Article

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    SP - 59

    EP - 65

    JO - Nutrition and Cancer - An International Journal

    T2 - Nutrition and Cancer - An International Journal

    JF - Nutrition and Cancer - An International Journal

    SN - 0163-5581

    IS - 1

    ER -

    Gill CIR, Heavey P, McConville E, Bradbury I, Faessler C, Mueller S et al. Effect of fecal water on an in vitro model of colonic mucosal barrier function. NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL. 2007;57(1):59-65.