Effect of cholesterol feeding on DNA damage in male and female Syrian hamsters

E Turley, NC Armstrong, JMW Wallace, WS Gilmore, VJ McKelvey-Martin, J Allen, JJ Strain

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Cholesterol oxides are cytotoxic and have been implicated in many disease processes; however, it has been proposed that cholesterol oxides result from cholesterol acting as a sacrificial antioxidant. In this study, the effect of dietary cholesterol on DNA damage, assessed by the alkaline comet assay, was examined in male and female Syrian hamsters. Animals were fed ad libitum a modified AIN-76 diet (control) or a diet with 0.5% cholesterol for 10 weeks. Following the 10-week feeding period, there was no significant difference in body weight between cholesterol-fed and control animals. Cholesterol feeding resulted in significant liver hypertrophy, and increased plasma total and HDL cholesterol in both male and female animals compared with controls. There was no difference in liver cell DNA damage levels as measured by the comet assay. Heart cells from cholesterol-fed hamsters, however, showed a significant decrease in tail DNA (p = 0.050) indicating decreased damage compared with controls and a possible protective effect of cholesterol against DNA damage.
LanguageEnglish
Pages47-51
JournalAnnals of Nutrition and Metabolism
Volume43
Issue number1
DOIs
Publication statusPublished - Jan 1999

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DNA damage
cholesterol
oxides
Mesocricetus auratus
assays
hypertrophy
hamsters
high density lipoprotein cholesterol
diet
hepatocytes
protective effect
animals
tail
heart
antioxidants
liver
body weight
DNA

Cite this

Turley, E., Armstrong, NC., Wallace, JMW., Gilmore, WS., McKelvey-Martin, VJ., Allen, J., & Strain, JJ. (1999). Effect of cholesterol feeding on DNA damage in male and female Syrian hamsters. Annals of Nutrition and Metabolism, 43(1), 47-51. https://doi.org/10.1159/000012766
Turley, E ; Armstrong, NC ; Wallace, JMW ; Gilmore, WS ; McKelvey-Martin, VJ ; Allen, J ; Strain, JJ. / Effect of cholesterol feeding on DNA damage in male and female Syrian hamsters. In: Annals of Nutrition and Metabolism. 1999 ; Vol. 43, No. 1. pp. 47-51.
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Turley, E, Armstrong, NC, Wallace, JMW, Gilmore, WS, McKelvey-Martin, VJ, Allen, J & Strain, JJ 1999, 'Effect of cholesterol feeding on DNA damage in male and female Syrian hamsters', Annals of Nutrition and Metabolism, vol. 43, no. 1, pp. 47-51. https://doi.org/10.1159/000012766

Effect of cholesterol feeding on DNA damage in male and female Syrian hamsters. / Turley, E; Armstrong, NC; Wallace, JMW; Gilmore, WS; McKelvey-Martin, VJ; Allen, J; Strain, JJ.

In: Annals of Nutrition and Metabolism, Vol. 43, No. 1, 01.1999, p. 47-51.

Research output: Contribution to journalArticle

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AB - Cholesterol oxides are cytotoxic and have been implicated in many disease processes; however, it has been proposed that cholesterol oxides result from cholesterol acting as a sacrificial antioxidant. In this study, the effect of dietary cholesterol on DNA damage, assessed by the alkaline comet assay, was examined in male and female Syrian hamsters. Animals were fed ad libitum a modified AIN-76 diet (control) or a diet with 0.5% cholesterol for 10 weeks. Following the 10-week feeding period, there was no significant difference in body weight between cholesterol-fed and control animals. Cholesterol feeding resulted in significant liver hypertrophy, and increased plasma total and HDL cholesterol in both male and female animals compared with controls. There was no difference in liver cell DNA damage levels as measured by the comet assay. Heart cells from cholesterol-fed hamsters, however, showed a significant decrease in tail DNA (p = 0.050) indicating decreased damage compared with controls and a possible protective effect of cholesterol against DNA damage.

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Turley E, Armstrong NC, Wallace JMW, Gilmore WS, McKelvey-Martin VJ, Allen J et al. Effect of cholesterol feeding on DNA damage in male and female Syrian hamsters. Annals of Nutrition and Metabolism. 1999 Jan;43(1):47-51. https://doi.org/10.1159/000012766