Effect of cellulose crystallinity modification by starch gel treatment for improvement in ethanol fermentation rate by non‑GM yeast cell factories

Iris Plioni; Archontoula Kalogeropoulou; Dimitra Dimitrellou; Panagiotis Kandylis; Maria Kanellaki; Poonam Singh - Nee Nigam; Athanasios Koutinas

doi:10.1007/s00449-022-02706-y

Effect of cellulose crystallinity modification by starch gel treatment for improvement in ethanol fermentation rate by non‑GM yeast cell factories

Iris Plioni, Archontoula Kalogeropoulou, Dimitra Dimitrellou, Panagiotis Kandylis, Maria Kanellaki, Poonam Singh - Nee Nigam, Athanasios Koutinas

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2 Citations (Scopus)

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Abstract

This paper studies the reduction of crystallinity degree (CD) of cellulose treated with starch gel (SG), and the correlation of CD with the fermentation efficiency of cellulose to fuel-grade ethanol. Cellulose bioconversion from wood sawdust, consisting of three processes, was conducted in the same batch (one-step). The XRD and TEM analysis revealed 11% reduction in cellulose CD after its treatment with SG. One-step bioconversion process was performed employing two cell factories (CF) of non-engineered S. cerevisiae. CFs contained non- engineered S. cerevisiae cells covered with either SG entrapping Trichoderma reesei or cellulases prepared in the laboratory and immobilized in SG. The consolidated fermentation of treated cellulose resulted in an increase of bioethanol concentration (60–90%) in 2-day fermentation and the maximum ethanol concentration reached was approximately 5 mL/L (3.95 g/L). The fermentation efficiency for grade-fuel ethanol production was improved by cellulose pretreatment using SG to achieve reduced CD.

Original language	English
Pages (from-to)	783-790
Number of pages	8
Journal	Bioprocess and Biosystems Engineering
Volume	45
Issue number	4
Early online date	21 Feb 2022
DOIs	https://doi.org/10.1007/s00449-022-02706-y
Publication status	Published (in print/issue) - 1 Apr 2022

Bibliographical note

Funding Information:
The authors acknowledge support of this work by the project “Research Infrastructure on Food Bioprocessing Development and Innovation Exploitation—Food Innovation RI” (MIS 5027222), which was implemented under the Action “Reinforcement of the Research and Innovation Infrastructure”, funded by the Operational Program “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014-2020) and co-financed by Greece and the European Union (European Regional Development Fund).

Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Keywords

Cellulose
Crystallinity
Cell factory
Cellulases
Saccharomyces

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s00449-022-02706-y

2022_Accepted_crystallinity_Manuscript_BBEAuthor Accepted Manuscript, 51.2 KB

Cite this

Plioni, I., Kalogeropoulou, A., Dimitrellou, D., Kandylis, P., Kanellaki, M., Singh - Nee Nigam, P., & Koutinas, A. (2022). Effect of cellulose crystallinity modification by starch gel treatment for improvement in ethanol fermentation rate by non‑GM yeast cell factories. Bioprocess and Biosystems Engineering, 45(4), 783-790. https://doi.org/10.1007/s00449-022-02706-y

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abstract = "This paper studies the reduction of crystallinity degree (CD) of cellulose treated with starch gel (SG), and the correlation of CD with the fermentation efficiency of cellulose to fuel-grade ethanol. Cellulose bioconversion from wood sawdust, consisting of three processes, was conducted in the same batch (one-step). The XRD and TEM analysis revealed 11% reduction in cellulose CD after its treatment with SG. One-step bioconversion process was performed employing two cell factories (CF) of non-engineered S. cerevisiae. CFs contained non- engineered S. cerevisiae cells covered with either SG entrapping Trichoderma reesei or cellulases prepared in the laboratory and immobilized in SG. The consolidated fermentation of treated cellulose resulted in an increase of bioethanol concentration (60–90%) in 2-day fermentation and the maximum ethanol concentration reached was approximately 5 mL/L (3.95 g/L). The fermentation efficiency for grade-fuel ethanol production was improved by cellulose pretreatment using SG to achieve reduced CD.",

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note = "Funding Information: The authors acknowledge support of this work by the project “Research Infrastructure on Food Bioprocessing Development and Innovation Exploitation—Food Innovation RI” (MIS 5027222), which was implemented under the Action “Reinforcement of the Research and Innovation Infrastructure”, funded by the Operational Program “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014-2020) and co-financed by Greece and the European Union (European Regional Development Fund). Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.",

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Plioni, I, Kalogeropoulou, A, Dimitrellou, D, Kandylis, P, Kanellaki, M, Singh - Nee Nigam, P & Koutinas, A 2022, 'Effect of cellulose crystallinity modification by starch gel treatment for improvement in ethanol fermentation rate by non‑GM yeast cell factories', Bioprocess and Biosystems Engineering, vol. 45, no. 4, pp. 783-790. https://doi.org/10.1007/s00449-022-02706-y

Effect of cellulose crystallinity modification by starch gel treatment for improvement in ethanol fermentation rate by non‑GM yeast cell factories. / Plioni, Iris; Kalogeropoulou, Archontoula; Dimitrellou, Dimitra et al.
In: Bioprocess and Biosystems Engineering, Vol. 45, No. 4, 01.04.2022, p. 783-790.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Effect of cellulose crystallinity modification by starch gel treatment for improvement in ethanol fermentation rate by non‑GM yeast cell factories

AU - Plioni, Iris

AU - Kalogeropoulou, Archontoula

AU - Dimitrellou, Dimitra

AU - Kandylis, Panagiotis

AU - Kanellaki, Maria

AU - Singh - Nee Nigam, Poonam

AU - Koutinas, Athanasios

N1 - Funding Information: The authors acknowledge support of this work by the project “Research Infrastructure on Food Bioprocessing Development and Innovation Exploitation—Food Innovation RI” (MIS 5027222), which was implemented under the Action “Reinforcement of the Research and Innovation Infrastructure”, funded by the Operational Program “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014-2020) and co-financed by Greece and the European Union (European Regional Development Fund). Publisher Copyright: © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

PY - 2022/4/1

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N2 - This paper studies the reduction of crystallinity degree (CD) of cellulose treated with starch gel (SG), and the correlation of CD with the fermentation efficiency of cellulose to fuel-grade ethanol. Cellulose bioconversion from wood sawdust, consisting of three processes, was conducted in the same batch (one-step). The XRD and TEM analysis revealed 11% reduction in cellulose CD after its treatment with SG. One-step bioconversion process was performed employing two cell factories (CF) of non-engineered S. cerevisiae. CFs contained non- engineered S. cerevisiae cells covered with either SG entrapping Trichoderma reesei or cellulases prepared in the laboratory and immobilized in SG. The consolidated fermentation of treated cellulose resulted in an increase of bioethanol concentration (60–90%) in 2-day fermentation and the maximum ethanol concentration reached was approximately 5 mL/L (3.95 g/L). The fermentation efficiency for grade-fuel ethanol production was improved by cellulose pretreatment using SG to achieve reduced CD.

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KW - Cell factory

KW - Cellulases

KW - Saccharomyces

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U2 - 10.1007/s00449-022-02706-y

DO - 10.1007/s00449-022-02706-y

M3 - Article

SN - 1615-7591

VL - 45

SP - 783

EP - 790

JO - Bioprocess and Biosystems Engineering

JF - Bioprocess and Biosystems Engineering

IS - 4

ER -

Effect of cellulose crystallinity modification by starch gel treatment for improvement in ethanol fermentation rate by non‑GM yeast cell factories

Abstract

Bibliographical note

Keywords

UN SDGs

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