Effect of Base Sequence on G-Wire Formation in Solution

L Spindler, R Rigler, I Drevenˇsek-Olenik, N Ma’ani Hessari, Mateus Webba da Silva

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The formation and dimensions of G-wires by different short G-rich DNA sequences in solution were investigated by dynamiclight scattering (DLS) and polyacrilamide gel electrophoresis (PAGE). To explore the basic principles of wire formation, westudied the effects of base sequence, method of preparation, temperature, and oligonucleotide concentration. Both DLS andPAGE show that thermal annealing induces much less macromolecular self-assembly than dialysis. The degree of assembly andconsequently length of G-wires (5-6 nm) are well resolved by both methods for DNA sequences with intermediate length, whilesome discrepancies appear for the shortest and longest sequences. As expected, the longest DNA sequence gives the longestmacromolecular aggregates with a length of about 11 nm as estimated by DLS. The quadruplex topologies show no concentrationdependence in the investigated DNA concentration range (0.1 mM–0.4mM) and no structural change upon heating.
LanguageEnglish
JournalJournal of Nucleic Acids
Volume2010
Issue numberArticl
DOIs
Publication statusPublished - 22 Mar 2010

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DNA sequences
Wire
Scattering
Dialysis
Electrophoresis
Oligonucleotides
Self assembly
Gels
Topology
Annealing
Heating
DNA
Temperature

Cite this

Spindler, L., Rigler, R., Drevenˇsek-Olenik, I., Ma’ani Hessari, N., & Webba da Silva, M. (2010). Effect of Base Sequence on G-Wire Formation in Solution. Journal of Nucleic Acids, 2010(Articl). https://doi.org/10.4061/2010/431651
Spindler, L ; Rigler, R ; Drevenˇsek-Olenik, I ; Ma’ani Hessari, N ; Webba da Silva, Mateus. / Effect of Base Sequence on G-Wire Formation in Solution. In: Journal of Nucleic Acids. 2010 ; Vol. 2010, No. Articl.
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Spindler, L, Rigler, R, Drevenˇsek-Olenik, I, Ma’ani Hessari, N & Webba da Silva, M 2010, 'Effect of Base Sequence on G-Wire Formation in Solution', Journal of Nucleic Acids, vol. 2010, no. Articl. https://doi.org/10.4061/2010/431651

Effect of Base Sequence on G-Wire Formation in Solution. / Spindler, L; Rigler, R; Drevenˇsek-Olenik, I; Ma’ani Hessari, N; Webba da Silva, Mateus.

In: Journal of Nucleic Acids, Vol. 2010, No. Articl, 22.03.2010.

Research output: Contribution to journalArticle

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Spindler L, Rigler R, Drevenˇsek-Olenik I, Ma’ani Hessari N, Webba da Silva M. Effect of Base Sequence on G-Wire Formation in Solution. Journal of Nucleic Acids. 2010 Mar 22;2010(Articl). https://doi.org/10.4061/2010/431651