Effect of Air Plasma Processing on the Adsorption Behaviour of Bovine Serum Albumin on Spin-Coated PMMA Surfaces

C Liu, BJ Meenan

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

This paper reports the adsorption of Bovine Serum Albumin (BSA) onto Dielectric Barrier Discharge (DBD) processed Poly(methyl methacrylate) (PMMA) surfaces by a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) technique. The purpose is to study the influence of DBD processing on the nature and scale of BSA adsorption on PMMA surface in vitro. It was observed that DBD processing improves the surface wettability of PMMA film, a fact attributable to the changes in surface chemistry and topography. Exposure of the PMMA to Phosphate Buffed Saline (PBS) solution in the QCM-D systemresulted in surface adsorption which reaches an equilibrium after about 30 minutes for pristine PMMA, and 90 minutes for processed PMMA surface. Subsequent injection of BSA in PBS indicated that the protein is immediately adsorbed onto the PMMA surface. It was revealed that adsorption behaviour of BSA on pristine PMMA differs from that on processed PMMA surface. A slower adsorption kinetics was observed for pristine PMMA surface, whilst a quick adsorption kinetics for processed PMMA. Moreover, the dissipation shift of protein adsorption suggested that BSA forms a more rigid structure on pristine PMMA surface that on processed surface. These data suggest that changes in wettability and attendant chemical properties and surface texture of the PMMA surface may play a significant role in BSA adsorption process.Keywords: in vitro test, protein adsorption, surface modification, polymethylmethacrylate, QCM-DCopyright © 2008, Jilin University. Published by Elsevier Limited and Science Press. All rights reserved.
LanguageEnglish
Pages204-214
JournalJournal of Bionic Engineering
Volume5
Issue number3
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Plasma applications
Polymethyl Methacrylate
Bovine Serum Albumin
Polymethyl methacrylates
Adsorption
Air
Quartz Crystal Microbalance Techniques
Wettability
Quartz crystal microbalances
Proteins
Wetting
Phosphates
Rigid structures
Kinetics
Monitoring
Surface Properties
Surface topography
Processing
Surface chemistry
Chemical properties

Cite this

@article{ffa1de8e2e834b74bac5ce7aa6d8b224,
title = "Effect of Air Plasma Processing on the Adsorption Behaviour of Bovine Serum Albumin on Spin-Coated PMMA Surfaces",
abstract = "This paper reports the adsorption of Bovine Serum Albumin (BSA) onto Dielectric Barrier Discharge (DBD) processed Poly(methyl methacrylate) (PMMA) surfaces by a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) technique. The purpose is to study the influence of DBD processing on the nature and scale of BSA adsorption on PMMA surface in vitro. It was observed that DBD processing improves the surface wettability of PMMA film, a fact attributable to the changes in surface chemistry and topography. Exposure of the PMMA to Phosphate Buffed Saline (PBS) solution in the QCM-D systemresulted in surface adsorption which reaches an equilibrium after about 30 minutes for pristine PMMA, and 90 minutes for processed PMMA surface. Subsequent injection of BSA in PBS indicated that the protein is immediately adsorbed onto the PMMA surface. It was revealed that adsorption behaviour of BSA on pristine PMMA differs from that on processed PMMA surface. A slower adsorption kinetics was observed for pristine PMMA surface, whilst a quick adsorption kinetics for processed PMMA. Moreover, the dissipation shift of protein adsorption suggested that BSA forms a more rigid structure on pristine PMMA surface that on processed surface. These data suggest that changes in wettability and attendant chemical properties and surface texture of the PMMA surface may play a significant role in BSA adsorption process.Keywords: in vitro test, protein adsorption, surface modification, polymethylmethacrylate, QCM-DCopyright {\circledC} 2008, Jilin University. Published by Elsevier Limited and Science Press. All rights reserved.",
author = "C Liu and BJ Meenan",
note = "Reference text: References 1TE Creighton, Proteins: Structures and Molecular Properties, W H Freeman, New York (1993). 2Rudh M, Murray B. Prestudy: Monitoring of Adsorption of Beta Casein and Beta Lactoglobulin with the QCM-D Technique, Q-Sense AB, Goteborg, 2000, 15. 3SF Santos, D Zanette, H Fischer and R Itri, A systematic study of bovine serum albumin (BSA) and sodium dodecyl sulfate (SDS) interactions by surface tension and small angle X-ray scattering, Journal of Colloid and Interface Science 262 (2003), pp. 400–408. Article | PDF (274 K) | View Record in Scopus | Cited By in Scopus (69) 4RL Johnston, DJ Spalton, A Hussain and J Marshall, In vitro protein adsorption to 2 intraocular lens materials1, Journal of Cataract and Refractive Surgery 25 (1999), pp. 1109–1115. Article | PDF (251 K) | View Record in Scopus | Cited By in Scopus (32) 5F Hook and B Kasemo, Variations in coupled water, viscoelastic properties, and film thickness of a mefp-1 protein film during adsorption and cross-linking: A quartz crystal microbalance with dissipation monitoring, ellipsometry, and surface plasmon resonance study, Analytical Chemistry 73 (2001), pp. 5796–5804. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (298) 6P Rossini, P Colpo, G Ceccone, KD Jandt and F Rossi, Surfaces engineering of polymeric films for biomedical applications, Materials Science and Engineering C 23 (2003), pp. 353–358. Article | PDF (291 K) | View Record in Scopus | Cited By in Scopus (32) 7P Valette, M Thomas and P Dejardin, Adsorption of low molecular weight proteins to hemodialysis membranes: Experimental results and simulations, Biomaterials 20 (1999), pp. 1621–1634. Abstract | PDF (663 K) | View Record in Scopus | Cited By in Scopus (40) 8Hook F. Development of a Novel QCM Technique for Protein Adsorption Studies. PhD Thesis, Goteborg University, Sweden, 2001. 9T Snabe and SB Petersen, Lag phase and hydrolysis mechanisms of triacrycerol film lipolysis, Chemistry and Physics of Lipids 125 (2003), pp. 69–82. Article | PDF (399 K) | View Record in Scopus | Cited By in Scopus (6) 10KA Marx, T Zhou, A Montrone, H Schulze and SJ Braunhut, A quartz crystal microbalance cell biosensor: Detection of microtubule alterations in living cells at nm nocodazole concentrations, Biosensors and Bioelectronics 16 (2001), pp. 773–782. Article | PDF (623 K) | View Record in Scopus | Cited By in Scopus (50) 11LH Pope, S Allen, MC Savies, CJ Robert, SJB Tendler and PM Williams, Probing DND duplex formation and DND-drug interactions by the quartz crystal microbalance technique, Langmuir 17 (2001), pp. 8300–8304. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (29) 12FL Dickert, O Hayden, P Lieberzeit, C Haderspoeck, R Bindeus, C Palfinger and B Wirl, Nano- and micro-structuring of sensor materials –from molecule to cell detection, Synthetic Metals 138 (2003), pp. 65–69. Article | PDF (257 K) | View Record in Scopus | Cited By in Scopus (30) 13C Galli Marxer, M Collaud Coen and L Schlapbach, Study of adsorption and viscoelastic properties of proteins with a quartz crystal microbalance by measuring the oscillation amplitude, Journal of Colloid and Interface Science 261 (2003), pp. 291–298. Article | PDF (207 K) | View Record in Scopus | Cited By in Scopus (22) 14EME Kristensen, H Rensmo, R Larsson and H Siegbahn, Characterization of heparin surfaces using photoelectron spectroscopy and quartz crystal microbalance, Biomaterials 24 (2003), pp. 4153–4159. Article | PDF (260 K) | View Record in Scopus | Cited By in Scopus (14) 15C Fredriksson, S Khilman, B Kasemo and DM Steel, In vitro real-time characterization of cell attachment and spreading, Journal of Materials Science: Materials in Medicine 9 (1998), pp. 785–788. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (41) 16M Tanaka, A Mochizuki, T Shiroya, T Motomura, K Shimura, M Onishi and Y Okahata, Study on kinetics of early stage protein adsorption on poly(2-methoxyethylacrylate) (PMEA) surface, Colloids and Surfaces A: Physicochemical and Enginneering Aspects 203 (2002), pp. 195–204. Article | PDF (225 K) | View Record in Scopus | Cited By in Scopus (30) 17M Tanaka, A Mochizuki, T Motomura, K Shimura, M Ohishi and Y Okahata, In situ studies on protein adsorption onto a poly(2-methoxyethylate) surface by a quartz crystal microbalance, Colloids and Surfaces A: Physicochemical and Enginneering Aspects 193 (2001), pp. 145–152. Article | PDF (143 K) | View Record in Scopus | Cited By in Scopus (41) 18A Halperin and DE Leckband, From ship hulls to contact lenses: Repression of protein adsorption and the puzzle of PEO. Comptes Rendus de l'Academie des Sciences, Series 4, Physics 1 (2000), pp. 1171–1178. Abstract | PDF (223 K) | View Record in Scopus | Cited By in Scopus (15) 19L Szleifer, Polymers and proteins: Interactions at interfaces, Current Opinion in Solid State and Materials Science 2 (1997), pp. 337–344. 20G Nimeri, C Fredriksson, H Elwing, L Liu, M Rodahl and B Kasemo, Neutrophil interaction with protein-coated surfaces studied by an extended quartz crystal microbalance technique, Colloids and Surfaces B: Biointerfaces 11 (1998), pp. 255–264. Article | PDF (340 K) | View Record in Scopus | Cited By in Scopus (26) 21C Steinem, A Janshoff, J Wegener, WP Ulrich, W Willenbrink, M Sieber and HJ Galla, Impedance and shear wave resonance analysis of ligand-receptor interactions at functionalized surfaces and of cell monolayers, Biosensors and Bioelectronics 12 (1997), pp. 787–808. Abstract | PDF (1627 K) | View Record in Scopus | Cited By in Scopus (41) 22MK Kim, IS Park, HD Park, WR Wee, JH Lee, KD Park, SH Kim and YH Kim, Effect of poly(ethylene glycol) graft polymerization of poly(methyl methacrylate) on cell adhesion: In vitro and in vivo study, Journal of Cataract and Refractive Surgery 27 (2001), pp. 766–774. Article | PDF (700 K) | View Record in Scopus | Cited By in Scopus (19) 23CZ Liu, JQ Wu, LQ Ren, J Tong, J Li, NY Cui, NMD Brown and BJ Meenan, Comparative study on the effect of RF and DBD plasma treatment on PTFE surface modification, Materials Chemistry and Physics 85 (2004), pp. 340–346. Article | PDF (346 K) | View Record in Scopus | Cited By in Scopus (12) 24CZ Liu, NY Cui, NMD Brown and BJ Meenan, Effect of DBD plasma operating parameters on the polymer surface modification, Surface and Coatings Technology 185 (2004), pp. 311–320. Article | PDF (329 K) | View Record in Scopus | Cited By in Scopus (25) 25G Borcia, NMD Brown, D Dixon and R McIlhagger, The effect of an air-dielectric barrier discharge on the surface properties and peel strength of medical packaging materials, Surface and Coatings Technology 179 (2003), pp. 70–77. 26G Borcia, NMD Brown and CA Anderson, The surface oxidation of selected polymers using an atmospheric pressure air dielectric barrier discharge, Part 2, Applied Surface Science 225 (2003), pp. 186–197. 27CZ Liu, NMD Brown and BJ Meenan, Statistical analysis of the effect of DBD operating parameters on the surface processing of PMMA film, Surface Science 575 (2005), pp. 273–286. Article | PDF (916 K) | View Record in Scopus | Cited By in Scopus (12) 28H Aizawa, S Kurosawa, M Tozuka, JW Park and K Kobayashi, Rapid detection of fibrinogen and fibrin degradation products using a smart QCM-sensor, Sensors and Actuators B: Chemical 101 (2004), pp. 150–154. Article | PDF (168 K) | View Record in Scopus | Cited By in Scopus (7) 29M Rodahl, F Hook, C Fredriksson, C Keller, A Krozer, P Brzezinski, MV Voinova and B Kasemo, Simultaneous frequency and dissipation factor QCM measurements of biomoleclar adsorption and cell adhesion, Faraday Discussion 107 (1997), pp. 229–246. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (180) 30S Ben Amor, G Baud, M Jacquet, G Nanse, P Fioux and M Nardin, XPS characterisation of plasma-treated and alumina-coated PMMA, Applied Surface Science 153 (2000), pp. 172–183. Article | PDF (305 K) | View Record in Scopus | Cited By in Scopus (25) 31U Konig, M Nitschke, M Pilz, F Simon, C Arnhold and C Werner, Stability and ageing of plasma treated PTFE surfaces, Colloids and Surfaces B: Biointerfaces 25 (2002), pp. 313–324. Article | PDF (579 K) | View Record in Scopus | Cited By in Scopus (14) 32T Hayakawa, M Yoshinari and K Nemoto, Characterization and protein-adsorption behaviour of deposited organic thin film onto titanium by plasma polymerization with hexamethyldisiloxane, Biomaterials 25 (2004), pp. 119–127. Article | PDF (376 K) | View Record in Scopus | Cited By in Scopus (25) 33F Hook, C Larsson and C Fant, Biofuncational surfaces studied by quartz crystal microbalance with dissipation monitoring. In: A Hubbard and P Somasundaran, Editors, Encydopedia of Surface and Colloid Science, Marcel Dekker Inc (2002). 34N Inagaki, K Narushima, SY Lim, YW Park and Y Ikeda, Surface modification of Ethylene-co-Tetrafluoroethylene films by remote plasmas, Journal of Polymer Science: Polymer Physics 40 (2002), pp. 2871–2882. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (15) 35H Kaczmarek, J Kowalonek, A Szalla and A Sionkowska, Surface modification of thin polymeric films by air-plasma or UV irradiation, Surface Science 507–510 (2002), pp. 883–888. Article | PDF (114 K) | View Record in Scopus | Cited By in Scopus (31) 36GS Oehrlein, Surface processes in low pressure plasmas, Surface Science 386 (1997), pp. 222–230. Article | PDF (785 K) | View Record in Scopus | Cited By in Scopus (39) 37SK Oiseth, A Krozer, B Kasemo and J Lausmaa, Surface modification of spin-coated high-density polyethylene films by argon and oxygen glow discharge plasma treatments, Applied Surface Science 202 (2002), pp. 92–103. Article | PDF (342 K) | View Record in Scopus | Cited By in Scopus (35) 38C Poleunis, C Rubio, C Compere and P Bertrand, Role of salts on the BSA adsorption on stainless steel in aqueous souutions. 2. ToF-SIMS spectral and chemical mapping study, Surface and Interface Analysis 34 (2002), pp. 55–58. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (6) 39JJ Gray, The interaction of proteins with solid surfaces, Current Opinion in Structural Biology 14 (2004), pp. 110–115. Article | PDF (129 K) | View Record in Scopus | Cited By in Scopus (193) 40V Hlady and J Buijs, Protein adsorption on solid surfaces, Current Opinion in Biotechnology 7 (1996), pp. 72–77. Abstract | PDF (536 K) | View Record in Scopus | Cited By in Scopus (104) 41M Malmsten, Formation of adsorbed protein layers, Journal of Colloid and Interface Science 207 (1998), pp. 186–199. Abstract | PDF (179 K) | View Record in Scopus | Cited By in Scopus (83) 42K Nakanishi, T Sakiyama and K Imamura, On the adsorption of proteins on solid surfaces, a common but very complicated phenomenon, Journal of Bioscience and Bioengineering 91 (2001), pp. 233–244. Abstract | PDF (1141 K) | Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (172) 43EN Pokidysheva, IA Maklakova, ZM Belomestnaya, NV Perova, SN Bagrov and VI Sevastianov, Comparative analysis of human serum albumin adsorption and complement activation for intraocular lenses, Artificial Organs 25 (2001), pp. 453–458. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (3)",
year = "2008",
month = "9",
doi = "10.1016/S1672-6529(08)60026-8",
language = "English",
volume = "5",
pages = "204--214",
journal = "Journal of Bionic Engineering",
issn = "1672-6529",
publisher = "Elsevier",
number = "3",

}

Effect of Air Plasma Processing on the Adsorption Behaviour of Bovine Serum Albumin on Spin-Coated PMMA Surfaces. / Liu, C; Meenan, BJ.

In: Journal of Bionic Engineering, Vol. 5, No. 3, 09.2008, p. 204-214.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of Air Plasma Processing on the Adsorption Behaviour of Bovine Serum Albumin on Spin-Coated PMMA Surfaces

AU - Liu, C

AU - Meenan, BJ

N1 - Reference text: References 1TE Creighton, Proteins: Structures and Molecular Properties, W H Freeman, New York (1993). 2Rudh M, Murray B. Prestudy: Monitoring of Adsorption of Beta Casein and Beta Lactoglobulin with the QCM-D Technique, Q-Sense AB, Goteborg, 2000, 15. 3SF Santos, D Zanette, H Fischer and R Itri, A systematic study of bovine serum albumin (BSA) and sodium dodecyl sulfate (SDS) interactions by surface tension and small angle X-ray scattering, Journal of Colloid and Interface Science 262 (2003), pp. 400–408. Article | PDF (274 K) | View Record in Scopus | Cited By in Scopus (69) 4RL Johnston, DJ Spalton, A Hussain and J Marshall, In vitro protein adsorption to 2 intraocular lens materials1, Journal of Cataract and Refractive Surgery 25 (1999), pp. 1109–1115. Article | PDF (251 K) | View Record in Scopus | Cited By in Scopus (32) 5F Hook and B Kasemo, Variations in coupled water, viscoelastic properties, and film thickness of a mefp-1 protein film during adsorption and cross-linking: A quartz crystal microbalance with dissipation monitoring, ellipsometry, and surface plasmon resonance study, Analytical Chemistry 73 (2001), pp. 5796–5804. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (298) 6P Rossini, P Colpo, G Ceccone, KD Jandt and F Rossi, Surfaces engineering of polymeric films for biomedical applications, Materials Science and Engineering C 23 (2003), pp. 353–358. Article | PDF (291 K) | View Record in Scopus | Cited By in Scopus (32) 7P Valette, M Thomas and P Dejardin, Adsorption of low molecular weight proteins to hemodialysis membranes: Experimental results and simulations, Biomaterials 20 (1999), pp. 1621–1634. Abstract | PDF (663 K) | View Record in Scopus | Cited By in Scopus (40) 8Hook F. Development of a Novel QCM Technique for Protein Adsorption Studies. PhD Thesis, Goteborg University, Sweden, 2001. 9T Snabe and SB Petersen, Lag phase and hydrolysis mechanisms of triacrycerol film lipolysis, Chemistry and Physics of Lipids 125 (2003), pp. 69–82. Article | PDF (399 K) | View Record in Scopus | Cited By in Scopus (6) 10KA Marx, T Zhou, A Montrone, H Schulze and SJ Braunhut, A quartz crystal microbalance cell biosensor: Detection of microtubule alterations in living cells at nm nocodazole concentrations, Biosensors and Bioelectronics 16 (2001), pp. 773–782. Article | PDF (623 K) | View Record in Scopus | Cited By in Scopus (50) 11LH Pope, S Allen, MC Savies, CJ Robert, SJB Tendler and PM Williams, Probing DND duplex formation and DND-drug interactions by the quartz crystal microbalance technique, Langmuir 17 (2001), pp. 8300–8304. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (29) 12FL Dickert, O Hayden, P Lieberzeit, C Haderspoeck, R Bindeus, C Palfinger and B Wirl, Nano- and micro-structuring of sensor materials –from molecule to cell detection, Synthetic Metals 138 (2003), pp. 65–69. Article | PDF (257 K) | View Record in Scopus | Cited By in Scopus (30) 13C Galli Marxer, M Collaud Coen and L Schlapbach, Study of adsorption and viscoelastic properties of proteins with a quartz crystal microbalance by measuring the oscillation amplitude, Journal of Colloid and Interface Science 261 (2003), pp. 291–298. Article | PDF (207 K) | View Record in Scopus | Cited By in Scopus (22) 14EME Kristensen, H Rensmo, R Larsson and H Siegbahn, Characterization of heparin surfaces using photoelectron spectroscopy and quartz crystal microbalance, Biomaterials 24 (2003), pp. 4153–4159. Article | PDF (260 K) | View Record in Scopus | Cited By in Scopus (14) 15C Fredriksson, S Khilman, B Kasemo and DM Steel, In vitro real-time characterization of cell attachment and spreading, Journal of Materials Science: Materials in Medicine 9 (1998), pp. 785–788. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (41) 16M Tanaka, A Mochizuki, T Shiroya, T Motomura, K Shimura, M Onishi and Y Okahata, Study on kinetics of early stage protein adsorption on poly(2-methoxyethylacrylate) (PMEA) surface, Colloids and Surfaces A: Physicochemical and Enginneering Aspects 203 (2002), pp. 195–204. Article | PDF (225 K) | View Record in Scopus | Cited By in Scopus (30) 17M Tanaka, A Mochizuki, T Motomura, K Shimura, M Ohishi and Y Okahata, In situ studies on protein adsorption onto a poly(2-methoxyethylate) surface by a quartz crystal microbalance, Colloids and Surfaces A: Physicochemical and Enginneering Aspects 193 (2001), pp. 145–152. Article | PDF (143 K) | View Record in Scopus | Cited By in Scopus (41) 18A Halperin and DE Leckband, From ship hulls to contact lenses: Repression of protein adsorption and the puzzle of PEO. Comptes Rendus de l'Academie des Sciences, Series 4, Physics 1 (2000), pp. 1171–1178. Abstract | PDF (223 K) | View Record in Scopus | Cited By in Scopus (15) 19L Szleifer, Polymers and proteins: Interactions at interfaces, Current Opinion in Solid State and Materials Science 2 (1997), pp. 337–344. 20G Nimeri, C Fredriksson, H Elwing, L Liu, M Rodahl and B Kasemo, Neutrophil interaction with protein-coated surfaces studied by an extended quartz crystal microbalance technique, Colloids and Surfaces B: Biointerfaces 11 (1998), pp. 255–264. Article | PDF (340 K) | View Record in Scopus | Cited By in Scopus (26) 21C Steinem, A Janshoff, J Wegener, WP Ulrich, W Willenbrink, M Sieber and HJ Galla, Impedance and shear wave resonance analysis of ligand-receptor interactions at functionalized surfaces and of cell monolayers, Biosensors and Bioelectronics 12 (1997), pp. 787–808. Abstract | PDF (1627 K) | View Record in Scopus | Cited By in Scopus (41) 22MK Kim, IS Park, HD Park, WR Wee, JH Lee, KD Park, SH Kim and YH Kim, Effect of poly(ethylene glycol) graft polymerization of poly(methyl methacrylate) on cell adhesion: In vitro and in vivo study, Journal of Cataract and Refractive Surgery 27 (2001), pp. 766–774. Article | PDF (700 K) | View Record in Scopus | Cited By in Scopus (19) 23CZ Liu, JQ Wu, LQ Ren, J Tong, J Li, NY Cui, NMD Brown and BJ Meenan, Comparative study on the effect of RF and DBD plasma treatment on PTFE surface modification, Materials Chemistry and Physics 85 (2004), pp. 340–346. Article | PDF (346 K) | View Record in Scopus | Cited By in Scopus (12) 24CZ Liu, NY Cui, NMD Brown and BJ Meenan, Effect of DBD plasma operating parameters on the polymer surface modification, Surface and Coatings Technology 185 (2004), pp. 311–320. Article | PDF (329 K) | View Record in Scopus | Cited By in Scopus (25) 25G Borcia, NMD Brown, D Dixon and R McIlhagger, The effect of an air-dielectric barrier discharge on the surface properties and peel strength of medical packaging materials, Surface and Coatings Technology 179 (2003), pp. 70–77. 26G Borcia, NMD Brown and CA Anderson, The surface oxidation of selected polymers using an atmospheric pressure air dielectric barrier discharge, Part 2, Applied Surface Science 225 (2003), pp. 186–197. 27CZ Liu, NMD Brown and BJ Meenan, Statistical analysis of the effect of DBD operating parameters on the surface processing of PMMA film, Surface Science 575 (2005), pp. 273–286. Article | PDF (916 K) | View Record in Scopus | Cited By in Scopus (12) 28H Aizawa, S Kurosawa, M Tozuka, JW Park and K Kobayashi, Rapid detection of fibrinogen and fibrin degradation products using a smart QCM-sensor, Sensors and Actuators B: Chemical 101 (2004), pp. 150–154. Article | PDF (168 K) | View Record in Scopus | Cited By in Scopus (7) 29M Rodahl, F Hook, C Fredriksson, C Keller, A Krozer, P Brzezinski, MV Voinova and B Kasemo, Simultaneous frequency and dissipation factor QCM measurements of biomoleclar adsorption and cell adhesion, Faraday Discussion 107 (1997), pp. 229–246. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (180) 30S Ben Amor, G Baud, M Jacquet, G Nanse, P Fioux and M Nardin, XPS characterisation of plasma-treated and alumina-coated PMMA, Applied Surface Science 153 (2000), pp. 172–183. Article | PDF (305 K) | View Record in Scopus | Cited By in Scopus (25) 31U Konig, M Nitschke, M Pilz, F Simon, C Arnhold and C Werner, Stability and ageing of plasma treated PTFE surfaces, Colloids and Surfaces B: Biointerfaces 25 (2002), pp. 313–324. Article | PDF (579 K) | View Record in Scopus | Cited By in Scopus (14) 32T Hayakawa, M Yoshinari and K Nemoto, Characterization and protein-adsorption behaviour of deposited organic thin film onto titanium by plasma polymerization with hexamethyldisiloxane, Biomaterials 25 (2004), pp. 119–127. Article | PDF (376 K) | View Record in Scopus | Cited By in Scopus (25) 33F Hook, C Larsson and C Fant, Biofuncational surfaces studied by quartz crystal microbalance with dissipation monitoring. In: A Hubbard and P Somasundaran, Editors, Encydopedia of Surface and Colloid Science, Marcel Dekker Inc (2002). 34N Inagaki, K Narushima, SY Lim, YW Park and Y Ikeda, Surface modification of Ethylene-co-Tetrafluoroethylene films by remote plasmas, Journal of Polymer Science: Polymer Physics 40 (2002), pp. 2871–2882. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (15) 35H Kaczmarek, J Kowalonek, A Szalla and A Sionkowska, Surface modification of thin polymeric films by air-plasma or UV irradiation, Surface Science 507–510 (2002), pp. 883–888. Article | PDF (114 K) | View Record in Scopus | Cited By in Scopus (31) 36GS Oehrlein, Surface processes in low pressure plasmas, Surface Science 386 (1997), pp. 222–230. Article | PDF (785 K) | View Record in Scopus | Cited By in Scopus (39) 37SK Oiseth, A Krozer, B Kasemo and J Lausmaa, Surface modification of spin-coated high-density polyethylene films by argon and oxygen glow discharge plasma treatments, Applied Surface Science 202 (2002), pp. 92–103. Article | PDF (342 K) | View Record in Scopus | Cited By in Scopus (35) 38C Poleunis, C Rubio, C Compere and P Bertrand, Role of salts on the BSA adsorption on stainless steel in aqueous souutions. 2. ToF-SIMS spectral and chemical mapping study, Surface and Interface Analysis 34 (2002), pp. 55–58. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (6) 39JJ Gray, The interaction of proteins with solid surfaces, Current Opinion in Structural Biology 14 (2004), pp. 110–115. Article | PDF (129 K) | View Record in Scopus | Cited By in Scopus (193) 40V Hlady and J Buijs, Protein adsorption on solid surfaces, Current Opinion in Biotechnology 7 (1996), pp. 72–77. Abstract | PDF (536 K) | View Record in Scopus | Cited By in Scopus (104) 41M Malmsten, Formation of adsorbed protein layers, Journal of Colloid and Interface Science 207 (1998), pp. 186–199. Abstract | PDF (179 K) | View Record in Scopus | Cited By in Scopus (83) 42K Nakanishi, T Sakiyama and K Imamura, On the adsorption of proteins on solid surfaces, a common but very complicated phenomenon, Journal of Bioscience and Bioengineering 91 (2001), pp. 233–244. Abstract | PDF (1141 K) | Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (172) 43EN Pokidysheva, IA Maklakova, ZM Belomestnaya, NV Perova, SN Bagrov and VI Sevastianov, Comparative analysis of human serum albumin adsorption and complement activation for intraocular lenses, Artificial Organs 25 (2001), pp. 453–458. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (3)

PY - 2008/9

Y1 - 2008/9

N2 - This paper reports the adsorption of Bovine Serum Albumin (BSA) onto Dielectric Barrier Discharge (DBD) processed Poly(methyl methacrylate) (PMMA) surfaces by a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) technique. The purpose is to study the influence of DBD processing on the nature and scale of BSA adsorption on PMMA surface in vitro. It was observed that DBD processing improves the surface wettability of PMMA film, a fact attributable to the changes in surface chemistry and topography. Exposure of the PMMA to Phosphate Buffed Saline (PBS) solution in the QCM-D systemresulted in surface adsorption which reaches an equilibrium after about 30 minutes for pristine PMMA, and 90 minutes for processed PMMA surface. Subsequent injection of BSA in PBS indicated that the protein is immediately adsorbed onto the PMMA surface. It was revealed that adsorption behaviour of BSA on pristine PMMA differs from that on processed PMMA surface. A slower adsorption kinetics was observed for pristine PMMA surface, whilst a quick adsorption kinetics for processed PMMA. Moreover, the dissipation shift of protein adsorption suggested that BSA forms a more rigid structure on pristine PMMA surface that on processed surface. These data suggest that changes in wettability and attendant chemical properties and surface texture of the PMMA surface may play a significant role in BSA adsorption process.Keywords: in vitro test, protein adsorption, surface modification, polymethylmethacrylate, QCM-DCopyright © 2008, Jilin University. Published by Elsevier Limited and Science Press. All rights reserved.

AB - This paper reports the adsorption of Bovine Serum Albumin (BSA) onto Dielectric Barrier Discharge (DBD) processed Poly(methyl methacrylate) (PMMA) surfaces by a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) technique. The purpose is to study the influence of DBD processing on the nature and scale of BSA adsorption on PMMA surface in vitro. It was observed that DBD processing improves the surface wettability of PMMA film, a fact attributable to the changes in surface chemistry and topography. Exposure of the PMMA to Phosphate Buffed Saline (PBS) solution in the QCM-D systemresulted in surface adsorption which reaches an equilibrium after about 30 minutes for pristine PMMA, and 90 minutes for processed PMMA surface. Subsequent injection of BSA in PBS indicated that the protein is immediately adsorbed onto the PMMA surface. It was revealed that adsorption behaviour of BSA on pristine PMMA differs from that on processed PMMA surface. A slower adsorption kinetics was observed for pristine PMMA surface, whilst a quick adsorption kinetics for processed PMMA. Moreover, the dissipation shift of protein adsorption suggested that BSA forms a more rigid structure on pristine PMMA surface that on processed surface. These data suggest that changes in wettability and attendant chemical properties and surface texture of the PMMA surface may play a significant role in BSA adsorption process.Keywords: in vitro test, protein adsorption, surface modification, polymethylmethacrylate, QCM-DCopyright © 2008, Jilin University. Published by Elsevier Limited and Science Press. All rights reserved.

U2 - 10.1016/S1672-6529(08)60026-8

DO - 10.1016/S1672-6529(08)60026-8

M3 - Article

VL - 5

SP - 204

EP - 214

JO - Journal of Bionic Engineering

T2 - Journal of Bionic Engineering

JF - Journal of Bionic Engineering

SN - 1672-6529

IS - 3

ER -