Ectopic leptin production by intraocular pancreatic islet organoids ameliorates the metabolic phenotype of ob/ob mice

Barbara Leibiger, Tilo Moede, Ismael Valladolid‐acebes, Meike Paschen, Montse Visa, Ingo B. Leibiger, Per Olof Berggren

Research output: Contribution to journalArticlepeer-review

Abstract

The pancreatic islets of Langerhans consist of endocrine cells that secrete peptide hormones into the blood circulation in response to metabolic stimuli. When transplanted into the anterior chamber of the eye (ACE), pancreatic islets engraft and maintain morphological features of native islets as well as islet‐specific vascularization and innervation patterns. In sufficient amounts, intraocular islets are able to maintain glucose homeostasis in diabetic mice. Islet organoids (pseudo-islets), which are formed by self‐reassembly of islet cells following disaggregation and genetic manipulation, behave similarly to native islets. Here, we tested the hypothesis that genetically engineered intraocular islet organoids can serve as production sites for leptin. To test this hypothesis, we chose the leptin‐deficient ob/ob mouse as a model system, which becomes severely obese, hyperinsulinemic, hyperglycemic, and insulin resistant. We generated a Tet‐OFF‐based beta‐cell‐specific adenoviral expression construct for mouse leptin, which allowed efficient transduction of native beta‐cells, optical monitoring of leptin expression by co‐expressed fluorescent proteins, and the possibility to switch‐off leptin expression by treatment with doxycycline. Intraocular transplantation of islet organoids formed from transduced islet cells, which lack functional leptin receptors, to ob/ob mice allowed optical monitoring of leptin expression and ameliorated their metabolic phenotype by improving bodyweight, glucose tolerance, serum insulin, and C‐peptide levels.

Original languageEnglish
Article number387
JournalMetabolites
Volume11
Issue number6
DOIs
Publication statusPublished - 14 Jun 2021

Keywords

  • Diabetes
  • Exocytosis
  • In vivo imaging
  • Leptin
  • Metabolism
  • Pancreatic islets
  • Tissue‐engineering
  • Transplantation
  • Viral transduction
  • β‐cell

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