Dual-mode refractive index and charge sensing to investigate complex surface chemistry on nanostructures

Nikhil Bhalla, Doojin Lee, Shivani Sathish, Amy Q. Shen

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This work presents a novel dual-mode charge and refractive index sensitive device integrated with nanoplasmonic islands, for the first time, on insulator-semiconductor junctions. The developed nano-metal-insulator semiconductor (nMIS) sensor facilitates simultaneous detection of charge and mass changes on the nanoislands due to the binding of biomolecules. The charging of the nanoislands is traced by using the capacitive field-effect electrolyte-metal-insulator-semiconductor structure and the refractive index changes are quantified by measuring the change in the localized surface plasmon resonances of the nanoislands. To demonstrate the performance of our dual-mode sensor we study the effect of oxygen plasma on immobilized biomolecules. As a case study biotinylated aptamers specific to interleukin 6 (IL-6) were chosen to conduct the immunoassay studies. We confirm that the adsorbed aptamers on the nanoislands do not lose their functionality after exposure to low energy oxygen plasma (<600 J). This finding is critical for the development of 'ready-to-use' microfluidic immunoassay platforms (glass-PDMS based) where immobilizing biomolecules on one of the substrates is often required prior to the bonding of glass and PDMS. Our results also open new opportunities for utilizing plasma to encapsulate biomolecules in polymers and hydrogels. More significantly, nMIS sensors can readily be adopted for multiplexed and high throughput label free immunoassay systems, further driving innovations in biomedical and healthcare research.

LanguageEnglish
Pages547-554
Number of pages8
JournalNanoscale
Volume9
Issue number2
Early online date17 Nov 2016
DOIs
Publication statusPublished - 14 Jan 2017

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Biomolecules
Surface chemistry
Nanostructures
Refractive index
Metals
Semiconductor materials
Plasmas
Sensors
Semiconductor junctions
Oxygen
Glass
Hydrogels
Surface plasmon resonance
Microfluidics
Electrolytes
Labels
Interleukin-6
Polymers
Innovation
Throughput

Cite this

Bhalla, Nikhil ; Lee, Doojin ; Sathish, Shivani ; Shen, Amy Q. / Dual-mode refractive index and charge sensing to investigate complex surface chemistry on nanostructures. In: Nanoscale. 2017 ; Vol. 9, No. 2. pp. 547-554.
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Dual-mode refractive index and charge sensing to investigate complex surface chemistry on nanostructures. / Bhalla, Nikhil; Lee, Doojin; Sathish, Shivani; Shen, Amy Q.

In: Nanoscale, Vol. 9, No. 2, 14.01.2017, p. 547-554.

Research output: Contribution to journalArticle

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