Do freshwater sponges facilitate the transfer of antibiotic resistance in water-borne Enterococcus faecalis isolated from wastewater?

Allison Cartwright, Victoria Daniels, Michael Conwell, Joerg Arnscheidt, James Dooley, Patrick Naughton, Chris McGonigle

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The study investigated if effluent from a municipal wastewater treatment plant contained high numbers of enterococci. It also tested if vancomycin resistance transfer between Enterococcus faecalis isolates from streams may be facilitated by freshwater sponges. A population of the freshwater sponge Ephydatia fluviatilis exists in the Cavan River (Ireland). The river section inhabited by these sponges receives effluents from wastewater treatment. Enterococcus numbers in treatment plant effluents exceeded those in samples taken upstream and downstream of the discharge point. Lower downstream numbers of Enterococcus were attributed to clearance by filterfeeding sponges and to streamwater dilution. The presence of Enterococcus bacteria throughout the whole course of the river was evidence for a multitude of faecal inputs. We tested effects of the presence of sponges on horizontal transfer of vancomycin resistance between riverine E. faecalis isolates. Filter mating experiments demonstrated increased transfer of vancomycin resistance between these bacteria strains as indicated by cultivation on antibiotic-selective plates after enterococci had been exposed to live or dead sponges. There was no significant difference in the number of bacteria with successful gene transfer between the treatments. The Irish river studied received enterococci not only through effluents from a municipal wastewater treatment plant, but also from many other sources. Facilitation of resistance transfer between enterococci strains appeared to be an effect of the presence of sponge tissue as a substrate rather than to rely on filter feeding activity.
LanguageEnglish
Title of host publicationUnknown Host Publication
Pages38-39
Number of pages2
Publication statusAccepted/In press - 3 Oct 2017
EventSfAM antimicrobial resistance conference 2017: Meeting the challenge - London
Duration: 3 Oct 2017 → …

Conference

ConferenceSfAM antimicrobial resistance conference 2017: Meeting the challenge
Period3/10/17 → …

Fingerprint

Enterococcus faecalis
Enterococcus
antibiotic resistance
wastewater
vancomycin
effluents
wastewater treatment
water
rivers
bacteria
gene transfer
Ireland
antibiotics

Keywords

  • Porifera
  • freshwater sponges
  • enterococci
  • wastewater
  • antibiotic resistance
  • antimicrobial resistance
  • Cavan

Cite this

@inproceedings{28589dc250cc4cb8858f80900ee135dd,
title = "Do freshwater sponges facilitate the transfer of antibiotic resistance in water-borne Enterococcus faecalis isolated from wastewater?",
abstract = "The study investigated if effluent from a municipal wastewater treatment plant contained high numbers of enterococci. It also tested if vancomycin resistance transfer between Enterococcus faecalis isolates from streams may be facilitated by freshwater sponges. A population of the freshwater sponge Ephydatia fluviatilis exists in the Cavan River (Ireland). The river section inhabited by these sponges receives effluents from wastewater treatment. Enterococcus numbers in treatment plant effluents exceeded those in samples taken upstream and downstream of the discharge point. Lower downstream numbers of Enterococcus were attributed to clearance by filterfeeding sponges and to streamwater dilution. The presence of Enterococcus bacteria throughout the whole course of the river was evidence for a multitude of faecal inputs. We tested effects of the presence of sponges on horizontal transfer of vancomycin resistance between riverine E. faecalis isolates. Filter mating experiments demonstrated increased transfer of vancomycin resistance between these bacteria strains as indicated by cultivation on antibiotic-selective plates after enterococci had been exposed to live or dead sponges. There was no significant difference in the number of bacteria with successful gene transfer between the treatments. The Irish river studied received enterococci not only through effluents from a municipal wastewater treatment plant, but also from many other sources. Facilitation of resistance transfer between enterococci strains appeared to be an effect of the presence of sponge tissue as a substrate rather than to rely on filter feeding activity.",
keywords = "Porifera, freshwater sponges, enterococci, wastewater, antibiotic resistance, antimicrobial resistance, Cavan",
author = "Allison Cartwright and Victoria Daniels and Michael Conwell and Joerg Arnscheidt and James Dooley and Patrick Naughton and Chris McGonigle",
note = "Reference text: Harwood, VJ, et al. (2014). Microbial source tracking markers for detection of fecal contamination in environmental waters: relationship between pathogens and human health outcomes. FEMS Microbiol Rev 38, 1-40 Berendonk, TU, et al. (2015). Tackling antibiotic resistance: the environmental framework. Nature Rev. Microbiology 13, 310-317 Zhang, R, et al. (2015). Antibacterial and residual antimicrobial activities against Enterococcus faecalis biofilm: a comparison between EDTA, chlorhexidine, cetrimide, MTAD and QMix. Scientific Reports 5, 1-5",
year = "2017",
month = "10",
day = "3",
language = "English",
pages = "38--39",
booktitle = "Unknown Host Publication",

}

Cartwright, A, Daniels, V, Conwell, M, Arnscheidt, J, Dooley, J, Naughton, P & McGonigle, C 2017, Do freshwater sponges facilitate the transfer of antibiotic resistance in water-borne Enterococcus faecalis isolated from wastewater? in Unknown Host Publication. pp. 38-39, SfAM antimicrobial resistance conference 2017: Meeting the challenge, 3/10/17.

Do freshwater sponges facilitate the transfer of antibiotic resistance in water-borne Enterococcus faecalis isolated from wastewater? / Cartwright, Allison; Daniels, Victoria; Conwell, Michael; Arnscheidt, Joerg; Dooley, James; Naughton, Patrick; McGonigle, Chris.

Unknown Host Publication. 2017. p. 38-39.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Do freshwater sponges facilitate the transfer of antibiotic resistance in water-borne Enterococcus faecalis isolated from wastewater?

AU - Cartwright, Allison

AU - Daniels, Victoria

AU - Conwell, Michael

AU - Arnscheidt, Joerg

AU - Dooley, James

AU - Naughton, Patrick

AU - McGonigle, Chris

N1 - Reference text: Harwood, VJ, et al. (2014). Microbial source tracking markers for detection of fecal contamination in environmental waters: relationship between pathogens and human health outcomes. FEMS Microbiol Rev 38, 1-40 Berendonk, TU, et al. (2015). Tackling antibiotic resistance: the environmental framework. Nature Rev. Microbiology 13, 310-317 Zhang, R, et al. (2015). Antibacterial and residual antimicrobial activities against Enterococcus faecalis biofilm: a comparison between EDTA, chlorhexidine, cetrimide, MTAD and QMix. Scientific Reports 5, 1-5

PY - 2017/10/3

Y1 - 2017/10/3

N2 - The study investigated if effluent from a municipal wastewater treatment plant contained high numbers of enterococci. It also tested if vancomycin resistance transfer between Enterococcus faecalis isolates from streams may be facilitated by freshwater sponges. A population of the freshwater sponge Ephydatia fluviatilis exists in the Cavan River (Ireland). The river section inhabited by these sponges receives effluents from wastewater treatment. Enterococcus numbers in treatment plant effluents exceeded those in samples taken upstream and downstream of the discharge point. Lower downstream numbers of Enterococcus were attributed to clearance by filterfeeding sponges and to streamwater dilution. The presence of Enterococcus bacteria throughout the whole course of the river was evidence for a multitude of faecal inputs. We tested effects of the presence of sponges on horizontal transfer of vancomycin resistance between riverine E. faecalis isolates. Filter mating experiments demonstrated increased transfer of vancomycin resistance between these bacteria strains as indicated by cultivation on antibiotic-selective plates after enterococci had been exposed to live or dead sponges. There was no significant difference in the number of bacteria with successful gene transfer between the treatments. The Irish river studied received enterococci not only through effluents from a municipal wastewater treatment plant, but also from many other sources. Facilitation of resistance transfer between enterococci strains appeared to be an effect of the presence of sponge tissue as a substrate rather than to rely on filter feeding activity.

AB - The study investigated if effluent from a municipal wastewater treatment plant contained high numbers of enterococci. It also tested if vancomycin resistance transfer between Enterococcus faecalis isolates from streams may be facilitated by freshwater sponges. A population of the freshwater sponge Ephydatia fluviatilis exists in the Cavan River (Ireland). The river section inhabited by these sponges receives effluents from wastewater treatment. Enterococcus numbers in treatment plant effluents exceeded those in samples taken upstream and downstream of the discharge point. Lower downstream numbers of Enterococcus were attributed to clearance by filterfeeding sponges and to streamwater dilution. The presence of Enterococcus bacteria throughout the whole course of the river was evidence for a multitude of faecal inputs. We tested effects of the presence of sponges on horizontal transfer of vancomycin resistance between riverine E. faecalis isolates. Filter mating experiments demonstrated increased transfer of vancomycin resistance between these bacteria strains as indicated by cultivation on antibiotic-selective plates after enterococci had been exposed to live or dead sponges. There was no significant difference in the number of bacteria with successful gene transfer between the treatments. The Irish river studied received enterococci not only through effluents from a municipal wastewater treatment plant, but also from many other sources. Facilitation of resistance transfer between enterococci strains appeared to be an effect of the presence of sponge tissue as a substrate rather than to rely on filter feeding activity.

KW - Porifera

KW - freshwater sponges

KW - enterococci

KW - wastewater

KW - antibiotic resistance

KW - antimicrobial resistance

KW - Cavan

M3 - Conference contribution

SP - 38

EP - 39

BT - Unknown Host Publication

ER -