Under conditions of oxidative stress damage can occur to all cellular biomolecules, including DNA. Such damage has been implicated in the pathogenesis and long-term complications of Type I diabetes. Levels of oxidative DNA damage in eight well controlled individuals with Type I diabetes (mean HbA1c 7.03 0.10) and eight age and gender matched control individuals (mean HbA1c 4.58 0.06) were compared using the modified comet assay. DNA strand breaks, endonuclease III (Endo III) and formamidopyrimidine glycosylase (FPG) sensitive-sites were measured. No significant difference was observed in the mean levels of oxidative DNA damage in the group with diabetes (mean Endo III 9.77 2.6; mean Fpg13.08 2.3) compared to the control group (mean Endo III 12.19 2.2; mean FPG12.48 1.4). However linear regression analysis revealed a statistically significant (p = 0.024) positive correlation between the number of FPG sensitive-sites and duration of Type I diabetes. These results indicate that even with good glycaemic control there was a positive correlation between levels of oxidative DNA damage and duration of Type I diabetes in vivo.
|Title of host publication||Unknown Host Publication|
|Number of pages||1|
|Publication status||Published (in print/issue) - 2011|
|Event||Diabetes and Obesity Conference by the ASO and Diabetes UK. - London|
Duration: 1 Jan 2011 → …
|Conference||Diabetes and Obesity Conference by the ASO and Diabetes UK.|
|Period||1/01/11 → …|